An injectable and fast-degradable poly(ethylene glycol) hydrogel fabricated via bioorthogonal strain-promoted azide–alkyne cycloaddition click chemistry

Jiang, Hua-Fang; Qin, Si‐Yong; Dong, Hui; Lei, Qi; Su, Xin; Zhuo, Ren‐Xi; Zhong, Zhiyuan

doi:10.1039/c5sm00508f

Cited by 59 publications

(35 citation statements)

References 42 publications

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“…Strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry has drawn increasing attention recently due to the mild reaction conditions and bioorthogonality (Xu et al, 2014 ). An injectable and degradable PEG-based hydrogel has been prepared via the bioorthogonal SPAAC click chemistry (Jiang et al, 2015 ). Michael addition reaction is the nucleophilic addition of a carbanion or a nucleophile to an α, β-unsaturated carbonyl compound.…”

Section: Materials and Crosslinking Methods For Functional Hydrogels mentioning

confidence: 99%

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

et al. 2018

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Tissue engineering (TE) has been used as an attractive and efficient process to restore the original tissue structures and functions through the combination of biodegradable scaffolds, seeded cells, and biological factors. As a unique type of scaffolds, hydrogels have been frequently used for TE because of their similar 3D structures to the native extracellular matrix (ECM), as well as their tunable biochemical and biophysical properties to control cell functions such as cell adhesion, migration, proliferation, and differentiation. Various types of hydrogels have been prepared from naturally derived biomaterials, synthetic polymers, or their combination, showing their promise in TE. This review summarizes the very recent progress of hydrogels used for TE applications. The strategies for tuning biophysical and biochemical properties, and structures of hydrogels are first introduced. Their influences on cell functions and promotive effects on tissue regeneration are then highlighted.

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Section: Materials and Crosslinking Methods For Functional Hydrogels mentioning

confidence: 99%

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

et al. 2018

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“…Jiang et al reported a fast degrading, injectable hydrogel system made from alkynemodified PEG and azide-modified PEG cross-linked via SP-AAC reaction. Their results showed excellent properties including in situ gel formation ability under in vivo conditions with mild immunogenic response [70]. Liu et al revealed poly(e-caprolactone)-based hyper-branched dendrimers which were cross-linked via SP-AAC reaction exhibiting high mechanical properties and biocompatibility.…”

Section: Strain-promoted Azide-alkyne Cycloaddition (Sp-aac)-based CLmentioning

confidence: 99%

Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications

Janarthanan

Noh

2018

Tissue Eng Regen Med

120

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BACKGROUND: The tissue engineering and regenerative medicine approach require biomaterials which are biocompatible, easily reproducible in less time, biodegradable and should be able to generate complex three-dimensional (3D) structures to mimic the native tissue structures. Click chemistry offers the much-needed multifunctional hydrogel materials which are interesting biomaterials for the tissue engineering and bioprinting inks applications owing to their excellent ability to form hydrogels with printability instantly and to retain the live cells in their 3D network without losing the mechanical integrity even under swollen state. METHODS: In this review, we present the recent developments of in situ hydrogel in the field of click chemistry reported for the tissue engineering and 3D bioinks applications, by mainly covering the diverse types of click chemistry methods such as Diels-Alder reaction, strain-promoted azide-alkyne cycloaddition reactions, thiol-ene reactions, oxime reactions and other interrelated reactions, excluding enzyme-based reactions. RESULTS: The click chemistry-based hydrogels are formed spontaneously on mixing of reactive compounds and can encapsulate live cells with high viability for a long time. The recent works reported by combining the advantages of click chemistry and 3D bioprinting technology have shown to produce 3D tissue constructs with high resolution using biocompatible hydrogels as bioinks and in situ injectable forms. CONCLUSION: Interestingly, the emergence of click chemistry reactions in bioink synthesis for 3D bioprinting have shown the massive potential of these reaction methods in creating 3D tissue constructs. However, the limitations and challenges involved in the click chemistry reactions should be analyzed and bettered to be applied to tissue engineering and 3D bioinks. The future scope of these materials is promising, including their applications in in situ 3D bioprinting for tissue or organ regeneration.

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“…12). SPAAC has been successfully applied to the preparation of injectable hydrogels [60] and cellencapsulating hydrogels. DeForest and Anseth have worked extensively on SPAAC crosslinking.…”

Section: Azide-alkyne Cycloadditionmentioning

confidence: 99%

Chemical cross-linking methods for cell encapsulation in hydrogels

Echalier

Valot

Martínez

et al. 2019

Materials Today Communications

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Cell-encapsulating hydrogels are of tremendous interest in regenerative medicine. Tissue engineering relies on biomaterials able to act as artificial extracellular matrices to guide cells towards the development of new tissues. Therefore, considerable efforts have been made to design biomaterials which mimic cells' native environment, thus encouraging natural behavior. The choice of biomaterial in which cells are embedded is crucial for their survival, proliferation and differentiation. Being more stable, chemical hydrogels are preferred over physical hydrogels as cell-laden substrates. When designing chemical hydrogels, scientists must choose not only the nature of the network (synthetic and/or biopolymers) but also the type of cross-link bridging hydrogel constituents. For that purpose, numerous chemistries have been used (i) to introduce reactive functions on the hydrogel precursors and (ii) to form covalent bonds in the presence of living cells. The review will discuss the advantages and limitations of each strategy.

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An injectable and fast-degradable poly(ethylene glycol) hydrogel fabricated via bioorthogonal strain-promoted azide–alkyne cycloaddition click chemistry

Cited by 59 publications

References 42 publications

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

Functional Hydrogels With Tunable Structures and Properties for Tissue Engineering Applications

Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications

Chemical cross-linking methods for cell encapsulation in hydrogels

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