An injectable chitosan-based hydrogel reinforced by oxidized nanocrystalline cellulose and mineral trioxide aggregate designed for tooth engineering applications

Abdolahinia, Elaheh Dalir; Alipour, Mahdieh; Aghazadeh, Marziyeh; Hassanpour, Mehdi; Ghorbani, Marjan; Aghazadeh, Zahra

doi:10.1007/s10570-022-04491-z

Cited by 8 publications

(5 citation statements)

References 61 publications

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“…The culture medium was replaced every three days. After 21 days, the Alizarin red staining was performed as mentioned previously 47 to measure the induction of mineralization in hDPSCs. Briefly, the wells were washed with PBS (three times), fixed with 4% formaldehyde for 20 min, and then washed with deionized water for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Synthesis, characterization, and evaluation of Hesperetin nanocrystals for regenerative dentistry

Alipour

Sharifi

Samiei

et al. 2023

Sci Rep

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Hesperetin (HS), a metabolite of hesperidin, is a polyphenolic component of citrus fruits. This ingredient has a potential role in bone strength and the osteogenic differentiation. The bone loss in the orofacial region may occur due to the inflammation response of host tissues. Nanotechnology applications have been harshly entered the field of regenerative medicine to improve the efficacy of the materials and substances. In the current study, the hesperetin nanocrystals were synthesized and characterized. Then, the anti-inflammatory and antioxidative effects of these nanocrystals were evaluated on inflamed human Dental Pulp Stem Cells (hDPSCs) and monocytes (U937). Moreover, the osteoinduction capacity of these nanocrystals was assessed by gene and protein expression levels of osteogenic specific markers including RUNX2, ALP, OCN, Col1a1, and BSP in hDPSCs. The deposition of calcium nodules in the presence of hesperetin and hesperetin nanocrystals was also assessed. The results revealed the successful fabrication of hesperetin nanocrystals with an average size of 100 nm. The levels of TNF, IL6, and reactive oxygen species (ROS) in inflamed hDPSCs and U937 significantly decreased in the presence of hesperetin nanocrystals. Furthermore, these nanocrystals induced osteogenic differentiation in hDPSCs. These results demonstrated the positive and effective role of fabricated nanocrystal forms of this natural ingredient for regenerative medicine purposes.

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Section: Methodsmentioning

confidence: 99%

Synthesis, characterization, and evaluation of Hesperetin nanocrystals for regenerative dentistry

Alipour

Sharifi

Samiei

et al. 2023

Sci Rep

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“…3 . The rheological behaviors mainly be influenced by the covalent bonds between the amino groups of SF and aldehyde functionality, electrostatic and hydrogen bonding between SF and ADCNCs, and the reinforcement in the network 42 . The results showed that G″ was lesser than G′ for the SF/ADCNCs hydrogels containing PEEK, which claims a stable crosslinked network enhanced quickly with the PEEK content.…”

Section: Resultsmentioning

confidence: 99%

“…Based on the results of the current study, injectable in situ forming hydrogels provide interconnected porous structures. This structure offers an appropriate surface for cell adhesion, nutrient and waste distribution 42 . Nontoxicity of fabricated hydrogels is another critical factor for developing biomaterials.…”

Section: Discussionmentioning

confidence: 99%

“…PEEK-containing hydrogels demonstrated higher OD values than ADCNCs/SF and the control group, which suggested higher mineralization potential of ADCNCs/SF/PEEK hydrogels. Anionic matrices in the scaffold structure is bounded to the secreted calcium ions for formation of nucliation niches 42 . The other studies evaluated calcium deposition in silk fibroin and cellulose nanocrystals hydrogels on human bone marrow mesenchymal cells and suggested that this backbone elevated calcium deposition and cytoskeleton formation 37 , 60 , 61 .…”

Section: Discussionmentioning

confidence: 99%

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A novel injectable hydrogel containing polyetheretherketone for bone regeneration in the craniofacial region

Alipour

Ghorbani

khatoonabad

et al. 2023

Sci Rep

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Polyetheretherketone (PEEK) is an organic material introduced as an alternative for titanium implants. Injectable hydrogels are the most promising approach for bone regeneration in the oral cavity to fill the defects with irregular shapes and contours conservatively. In the current study, injectable Aldehyde-cellulose nanocrystalline/silk fibroin (ADCNCs/SF) hydrogels containing PEEK were synthesized, and their bone regeneration capacity was evaluated. Structure, intermolecular interaction, and the reaction between the components were assessed in hydrogel structure. The cytocompatibility of the fabricated scaffolds was evaluated on human dental pulp stem cells (hDPSCs). Moreover, the osteoinduction capacity of ADCNCs/SF/PEEK hydrogels on hDPSCs was evaluated using Real-time PCR, Western blot, Alizarin red staining and ALP activity. Bone formation in critical-size defects in rats’ cranial was assessed histologically and radiographically. The results confirmed the successful fabrication of the hydrogel and its osteogenic induction ability on hDPSCs. Furthermore, in in vivo phase, bone formation was significantly higher in ADCNCs/SF/PEEK group. Hence, the enhanced bone regeneration in response to PEEK-loaded hydrogels suggested its potential for regenerating bone loss in the craniofacial region, explicitly surrounding the dental implants.

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“…In vitro (hDPSCs) Tooth (dentinpulp complex) [187] CNCs-Fbg injectable composite hydrogels In vitro (mouse myoblasts) Muscle (myotube formation) [188] CNCs-PVA composite hydrogels…”

Section: Cnfs-gel Crosslinked Hydrogelsmentioning

confidence: 99%

Insight into the Latest Medical Applications of Nanocellulose

Ghilan¹,

Nicu²,

Ciolacu³

et al. 2023

Materials

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Nanocelluloses (NCs) are appealing nanomaterials that have experienced rapid development in recent years, with great potential in the biomedical field. This trend aligns with the increasing demand for sustainable materials, which will contribute both to an improvement in wellbeing and an extension of human life, and with the demand to keep up with advances in medical technology. In recent years, due to the diversity of their physical and biological properties and the possibility of tuning them according to the desired goal, these nanomaterials represent a point of maximum interest in the medical field. Applications such as tissue engineering, drug delivery, wound dressing, medical implants or those in cardiovascular health are some of the applications in which NCs have been successfully used. This review presents insight into the latest medical applications of NCs, in the forms of cellulose nanocrystals (CNCs), cellulose nanofibers (CNFs) and bacterial nanocellulose (BNC), with an emphasis on the domains that have recently experienced remarkable growth, namely wound dressing, tissue engineering and drug delivery. In order to highlight only the most recent achievements, the presented information is focused on studies from the last 3 years. Approaches to the preparation of NCs are discussed either by top-down (chemical or mechanical degradation) or by bottom-up (biosynthesis) techniques, along with their morphological characterization and unique properties, such as mechanical and biological properties. Finally, the main challenges, limitations and future research directions of NCs are identified in a sustained effort to identify their effective use in biomedical fields.

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An injectable chitosan-based hydrogel reinforced by oxidized nanocrystalline cellulose and mineral trioxide aggregate designed for tooth engineering applications

Cited by 8 publications

References 61 publications

Synthesis, characterization, and evaluation of Hesperetin nanocrystals for regenerative dentistry

Synthesis, characterization, and evaluation of Hesperetin nanocrystals for regenerative dentistry

A novel injectable hydrogel containing polyetheretherketone for bone regeneration in the craniofacial region

Insight into the Latest Medical Applications of Nanocellulose

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