An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs)

Abstract

Although great efforts are being made using growth factors and gene therapy, the repair of bone defects remains a major challenge in modern medicine that has resulted in an increased burden on both healthcare and the economy. Emerging tissue engineering techniques that use of combination of biodegradable poly-lactic-co-glycolic acid (PLGA) and mesenchymal stem cells have shed light on improving bone defect healing; however, additional growth factors are also required with these methods. Therefore, the development of novel and cost-effective approaches is of great importance. Our in vitro results demonstrated that ESW treatment (10 kV, 500 pulses) has a stimulatory effect on the proliferation and osteogenic differentiation of bone marrow-derived MSCs (BMSCs). Histological and micro-CT results showed that PLGA scaffolds seeded with ESW-treated BMSCs produced more bone-like tissue with commitment to the osteogenic lineage when subcutaneously implanted in vivo, as compared to control group. Significantly greater bone formation with a faster mineral apposition rate inside the defect site was observed in the ESW group compared to control group. Biomechanical parameters, including ultimate load and stress at failure, improved over time and were superior to those of the control group. Taken together, this innovative approach shows significant potential in bone tissue regeneration.

Comparative evaluation of the osteogenic capacity of human mesenchymal stem cells from bone marrow and umbilical cord tissue

“…Tibia defect model. A total of 27 mature SD rats were used for the generation of the tibia bone defect model as described previously (19,20). In brief, general anesthesia was performed by administering sodium pentobarbital (40 mg/kg body weight; intraperitoneal injection; Merck KGaA), and a 1-2 cm skin incision was made to separate and expose the tibia following disinfection.…”

“…no. ab46154; 1:200; Abcam) were assessed according to protocols of previous studies (20,23). Immunohistochemistry was performed using the streptavidin-peroxidase method (SPlink Detection kit; cat.…”

“…ZLI-9019; OriGene Technologies, Inc.) was used for further staining at room temperature for 6 min. Finally, sections were counterstained with hematoxylin for 1 min at room temperature and observed under an optical microscope (magnification, x100; Nikon Ti-S; Nikon Corporation, Tokyo, Japan) (20). In order to semi-quantitatively analyze the immunoreactivity of OPN, COL-1 and VEGF, the H-score was determined.…”

Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds

“…They find a greater bone formation with a faster mineral apposition rate inside the defect site significantly in the ESW group. They believe that this innovative approach provide significant potential in bone tissue regeneration future (Chen et al, ). One of the major concerning in tissue engineering is neovascularization and soft tissue engineering.…”

Ударно-Волновая Терапия: Состояние Проблемы И Возможности Применения В Клинической Практике

“…Другим важным биологическим эффектом УВ является их активирующее влияние на обменные процессы тканевого и клеточного уровней [30,34,55]. В опытах на крысах было отмечено уменьшение размеров раны за счет быстрого раз-вития грануляционной ткани и ускоренной пол-ной эпителизации.…”