An innovative bioprocess for methane conversion to methanol using an efficient methane transfer chamber coupled with an airlift bioreactor

Ghaz-Jahanian, Mohammad Ali; Khoshfetrat, Ali Baradar; Rostami, Maliheh Hosseinian; Haghighi, Mohammad

doi:10.1016/j.cherd.2018.03.039

Cited by 22 publications

(5 citation statements)

References 40 publications

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“…et al 13 High-pressure batch reactor with sparger modifications Maximum methanol titer of 1.98 g/L was achieved under an elevated operating pressure of 4 bar in a high-pressure stirred tank reactor. 1.…”

Section: Ghaz-jahanianmentioning

confidence: 99%

Ultrasound Induced Enhancement of Biological Gas-to-Liquid Process for Methanol Synthesis from Methane Using Methylotuvimicrobium buryatense 5GB1C

Priyadarsini,

Khaire,

Chauhan

et al. 2024

ACS Sustainable Resour. Manage.

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Methanol is a potential alternate liquid transportation fuel. Conventional processes for methanol production from methane are energy-intensive. Microbial conversion of methane to methanol (Biological Gas-To-Liquid process) is a potential eco-friendly alternative. In this study, we have reported the intensification of methane fermentation to methanol (24 h batch mode) by Methylotuvimicrobium buryatense 5GB1C using 33 kHz sonication. The fermentation process was optimized for the sonication treatment time and duty cycle. A maximum titer of 20 mM (127.5 mg methanol/g dry cell weight biomass) was obtained in a 10 h sonication treatment at a 10% duty cycle, which was ∼57% higher than in control experiments. A mechanistic study of this result using pmoA gene expression (measured using qRT-PCR) and total protein analysis (sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)) revealed that the overexpression of the pmoA gene (therefore, pmoCAB operon) led to the overexpression of the particulate methane monooxygenase (pMMO) enzyme in the metabolic pathway of M. buryatense resulting in the production of pMMO in higher quantities than that in control experiments. The ultimate manifestation of these phenomena was faster enzyme kinetics and high methanol yield.

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Section: Ghaz-jahanianmentioning

confidence: 99%

Ultrasound Induced Enhancement of Biological Gas-to-Liquid Process for Methanol Synthesis from Methane Using Methylotuvimicrobium buryatense 5GB1C

Priyadarsini,

Khaire,

Chauhan

et al. 2024

ACS Sustainable Resour. Manage.

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“…Volumetric mass-transfer coefficient (K L a) is a major metric for the performance evaluation of different reactor configurations. Airlift bioreactors have also been reported for high K L a values of upto 97.2 h –1 ( Ghaz-Jahanian et al, 2018 ). Authors have reported the application of membrane biofilm reactors (MBR) for methanotroph-based bioremediation.…”

Section: Mass Transfer and Solubility Limitations In Methanotroph Basmentioning

confidence: 99%

Biotransformation of Methane and Carbon Dioxide Into High-Value Products by Methanotrophs: Current State of Art and Future Prospects

2021

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Conventional chemical methods to transform methane and carbon dioxide into useful chemicals are plagued by the requirement for extreme operating conditions and expensive catalysts. Exploitation of microorganisms as biocatalysts is an attractive alternative to sequester these C1 compounds and convert them into value-added chemicals through their inherent metabolic pathways. Microbial biocatalysts are advantageous over chemical processes as they require mild-operating conditions and do not release any toxic by-products. Methanotrophs are potential cell-factories for synthesizing a wide range of high-value products via utilizing methane as the sole source of carbon and energy, and hence, serve as excellent candidate for methane sequestration. Besides, methanotrophs are capable of capturing carbon dioxide and enzymatically hydrogenating it into methanol, and hence qualify to be suitable candidates for carbon dioxide sequestration. However, large-scale production of value-added products from methanotrophs still presents an overwhelming challenge, due to gas-liquid mass transfer limitations, low solubility of gases in liquid medium and low titer of products. This requires design and engineering of efficient reactors for scale-up of the process. The present review offers an overview of the metabolic architecture of methanotrophs and the range of product portfolio they can offer. Special emphasis is given on methanol biosynthesis as a potential biofuel molecule, through utilization of methane and alternate pathway of carbon dioxide sequestration. In view of the gas-liquid mass transfer and low solubility of gases, the key rate-limiting step in gas fermentation, emphasis is given toward reactor design consideration essential to achieve better process performance.

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“…Additional studies are needed to design and operate a reactor that can circulate the produced methane and CO 2 . Previous studies employing the internal [39] and external [40] loop airlift bioreactor methods for the circulation and use of methane in the manufacture of effective substances, such as methanol, by methanotrophs may provide an important basis for the development of optimal reactors.…”

Section: Interactions and Roles Of Bacterial Strains In The Biologica...mentioning

confidence: 99%

An Experimental Study on the Biological Fixation and Effective Use of Carbon Using Biogas and Bacterial Community Dominated by Methanotrophs, Methanol-Oxidizing Bacteria, and Ammonia-Oxidizing Bacteria

et al. 2021

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This study used biogas from a wastewater treatment plant and bacterial community where methanotrophs, Methylophilus, and ammonia-oxidizing bacteria clusters coexisted to propose an effective method for biological carbon fixation and nitrogen removal in wastewater treatment for carbon capture, utilization, and storage (CCUS). Biogas from wastewater treatment plant was provided, instead of purified CH4, to provide operation and maintenance conditions of bio-catalyst reaction for efficient biological carbon fixation by bacterial community using methane and CO2. This study assessed the conditions to induce type X methanotrophs that can use CO2 as a carbon source, as dominant species in the bacterial community and continuously and effectively supply reducing equivalents required for the conversion of CO2 to methanol within the system. Herein, the results of inducing efficient co-existence of methanotrophs, Methylophilus, and ammonia-oxidizing bacteria cluster in the bacterial community were shown.

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An innovative bioprocess for methane conversion to methanol using an efficient methane transfer chamber coupled with an airlift bioreactor

Cited by 22 publications

References 40 publications

Ultrasound Induced Enhancement of Biological Gas-to-Liquid Process for Methanol Synthesis from Methane Using Methylotuvimicrobium buryatense 5GB1C

Ultrasound Induced Enhancement of Biological Gas-to-Liquid Process for Methanol Synthesis from Methane Using Methylotuvimicrobium buryatense 5GB1C

Biotransformation of Methane and Carbon Dioxide Into High-Value Products by Methanotrophs: Current State of Art and Future Prospects

An Experimental Study on the Biological Fixation and Effective Use of Carbon Using Biogas and Bacterial Community Dominated by Methanotrophs, Methanol-Oxidizing Bacteria, and Ammonia-Oxidizing Bacteria

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