Coking wastewater contains high concentration of nitrogen compounds and carbon materials 1. Especially, with increasing of ammonium-nitrogen concentration in cokes wastewater, numerous environmental and health problems will be serious if not treated effectively 2. Nitrogen removal is an important aspect of present wastewater treatment system. The most common, efficient and cost effective methods involve heterotrophic nitrifiers and aerobic denitrifiers to remove ammoniumnitrogen 3. Recently, studies have highlighted the existence of some bacteria such as Paracoccus denitrificans 4 , Pseudomonas denitrificans 5 , Bacterium 6 and Alcaligenes faecalis 7. However, studies on these bacteria have focused on a low ammonium concentration while research on the treatment of high-strength ammonium wastewater is rare 8. Compared with bacteria, filamentous fungi are adsorbed with each other, easy to be got and immobilized after cultivating 9. Fungi, such as Phanerochaete chrysosporium 10 and Penicillium, have made contribution to remove metalion, organic compound and pigments in wastewater. But available fungi used to remove high-strength ammonium-nitrogen are few 11. Based on above, further research is focused on screening for fungi with high-strength ammonium removal efficiency. In this study, a Fusarium sp was isolated from cokes wastewater