An insight into the genotoxicity assessment studies in dipterans

Mishra, Nidhi; Srivastava, R. C.; Agrawal, Uma Rani; Tewari, Raghav Ram

doi:10.1016/j.mrrev.2016.10.001

Cited by 10 publications

(6 citation statements)

References 178 publications

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“…Cyclophosphamide, TEPA, thio-TEPA and analogs are known as chemosterilants and genotoxic to insects, and may be used for their control. [7,8] The polar head group of N-substituted P=O was used as the hydrophilic fragment in nonionic fluorinated amphiphiles designed for drug delivery and biocompatible oxygen transport. The specific structures involved partially fluorinated, long alkoxy chains for X, and (R 2 N) = morpholino and n = 1, 2.…”

Section: Introductionmentioning

confidence: 99%

On the Lewis Basicity of Phosphoramides: A Critical Examination of Their Donor Number through Comparison of Enthalpies of Adduct Formation with SbCl₅and BF₃

et al. 2019

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The Lewis basicity of a series of phosphoryl compounds was examined using DFT and ab initio methods, including solvation effects. The enthalpies of adduct formation with two archetypal Lewis acids, antimony pentachloride and boron trifluoride, used to define the donor number DN and the BF3 affinity (BF3A) respectively, were examined. The BF3 adducts allow the use of the high‐accuracy G4 approach, whereas for SbCl5 adducts, three different DFT formalisms, including empirical dispersion corrections, were used because the G4 formalism is not available for third‐row elements. For a comparison with experimental data, solvation effects were taken into account by using the polarizable continuum model. The experimental BF3 affinities were well reproduced by G4 calculations when including PCM solvation. Conversely, comparisons of our calculated values and experimental results reported in the literature show that SbCl5 enthalpies for phosphoramides are in error. In particular the DN for HMPA should be revised.

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Section: Introductionmentioning

confidence: 99%

On the Lewis Basicity of Phosphoramides: A Critical Examination of Their Donor Number through Comparison of Enthalpies of Adduct Formation with SbCl₅and BF₃

et al. 2019

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“…Drosophila melanogaster is currently being used as one of the preferable organisms for toxicological research [2]. According to current knowledge, the use of D. melanogaster as a model organism respects the principles of animal welfare (3Rs), since ethical matters do not urge when using this organism [2,3].…”

Section: Drosophila Melanogaster In Toxicological Researchmentioning

confidence: 99%

“…According to current knowledge, the use of D. melanogaster as a model organism respects the principles of animal welfare (3Rs), since ethical matters do not urge when using this organism [2,3]. Considering the metabolic pathways responsible for dietary input (including nutrient uptake, digestion, absorption, storage and metabolism) [4], the xenobiotic metabolizing system, the antioxidant enzymes and the DNA repair systems of D. melanogaster, which are analogous to those of mammals, D. melanogaster emerges as an optimal replacer of higher animals in toxicological studies [2,5]. Furthermore, contrasting with in vitro methods, D. melanogaster has the advantage of enabling a more solid extrapolation at the organism level [3].…”

Section: Drosophila Melanogaster In Toxicological Researchmentioning

confidence: 99%

“…In this way, genotoxicological studies with D. melanogaster deal with the assessment of changes in genetic material through various assays, such as germ line mutation assays, somatic mutation assays, the chromosomal aberration assay, the micronucleus test, the comet assay and DNA sequence-based assays, among others. In particular, somatic mutation and recombination tests (SMARTs) have proven to be a good tool for detecting a broad range of genetic alterations quickly and inexpensively [2,8].…”

Section: Drosophila Melanogaster In Toxicological Researchmentioning

confidence: 99%

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The w/w ⁺ Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster for Detecting Antigenotoxic Activity

Gaivão¹,

Ferreira²,

Sierra³

2021

Genotoxicity and Mutagenicity - Mechanisms and Test Methods

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Genotoxicological studies are emerging as fundamental for knowing the hazards to our genome, to our health. Drosophila melanogaster is one of the preferable organisms for toxicological research considering its metabolic similarities (viz. on dietary input, xenobiotic metabolizing system, antioxidant enzymes and DNA repair systems) to mammals. Accordingly, somatic mutation and recombination tests (SMARTs) of D. melanogaster are fast and low-cost in vivo assays that have shown solid results evaluating genotoxicity. The w/w + SMART uses the white (w) gene as a recessive marker to monitor the presence of mutant ommatidia (eye units), indicating the occurrence of point mutations, deletions, mitotic recombination or/and nondisjunction. Additionally, several studies used SMARTs to assess antigenotoxicity, with some using the w/w + SMART. We reviewed the state of the art of the w/w + SMART used for antigenotoxicity analysis, focusing on published results, aiming to contribute to the conception of a reliable protocol in antigenotoxicity. As such, genotoxic agents with known action mechanisms, as streptonigrin (oxidative stress inducer), were used as a genotoxic insult for proving the antigenotoxic effects of natural substances (e.g. seaweeds), demonstrating the presence of antimutagens in their composition. These antigenotoxicity studies are crucial for promoting preventive measures against environmental genotoxics that affect humans daily.

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“…Short life span, easy maintenance, the production of a large number of offspring in a single generation and tissues with appropriate cell populations make them ideal for studies in developmental biology, diseases, genetics, genetic toxicology and stress biology. Besides, their cosmopolitan presence makes them suitable candidates for ecological biomonitoring [317].…”

Section: Insectsmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview on the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, molluscs, annelids, arthropods and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.

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An insight into the genotoxicity assessment studies in dipterans

Cited by 10 publications

References 178 publications

On the Lewis Basicity of Phosphoramides: A Critical Examination of Their Donor Number through Comparison of Enthalpies of Adduct Formation with SbCl₅and BF₃

On the Lewis Basicity of Phosphoramides: A Critical Examination of Their Donor Number through Comparison of Enthalpies of Adduct Formation with SbCl₅and BF₃

The w/w ⁺ Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster for Detecting Antigenotoxic Activity

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

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An insight into the genotoxicity assessment studies in dipterans

Cited by 10 publications

References 178 publications

On the Lewis Basicity of Phosphoramides: A Critical Examination of Their Donor Number through Comparison of Enthalpies of Adduct Formation with SbCl5and BF3

On the Lewis Basicity of Phosphoramides: A Critical Examination of Their Donor Number through Comparison of Enthalpies of Adduct Formation with SbCl5and BF3

The w/w + Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster for Detecting Antigenotoxic Activity

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

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On the Lewis Basicity of Phosphoramides: A Critical Examination of Their Donor Number through Comparison of Enthalpies of Adduct Formation with SbCl₅and BF₃

On the Lewis Basicity of Phosphoramides: A Critical Examination of Their Donor Number through Comparison of Enthalpies of Adduct Formation with SbCl₅and BF₃

The w/w ⁺ Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster for Detecting Antigenotoxic Activity