An insight on lipid nanoparticles for therapeutic proteins delivery

Viegas, Cláudia Marina; Seck, Fatumata; Fonte, Pedro

doi:10.1016/j.jddst.2022.103839

Cited by 25 publications

(21 citation statements)

References 105 publications

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“…We suggest that the rNTD-S1 protein interacts with the nanoparticles via adsorption, due to its pI of 7.59 and the Tris-HCl buffer (140 mM, pH 7.4) in which the nanoparticles were synthesized, since previous reports showed that at pH values closer to the pI the interactions between the proteins themselves are reduced, thus increasing the adsorption of the proteins towards the nanoparticles [ 23 ]. In addition, the charge of the NTD-S1 protein is slightly positive (2.11 mV), which favors the interactions with nanoparticles composed of phospholipids, which are negatively charged by nature and whose interaction takes place in the hydrophilic region of the lipid aggregates [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…Studies on liposome-based veterinary vaccines have markedly increased, and pig vaccination has become an essential part of pig production; viral vaccines are essential tools for pig producers and veterinarians to manage pig herd health [ 22 ]. The ability to induce strong immune responses provided by co-formulated adjuvants, such as liposome-based vaccines, is critical for the development of modern vaccine formulations [ 23 ]. This study focused on improving current trends and implementing new technologies for the development of novel porcine virus vaccines.…”

Section: Introductionmentioning

confidence: 99%

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Development of Glycyrrhizinic Acid-Based Lipid Nanoparticle (LNP-GA) as An Adjuvant That Improves the Immune Response to Porcine Epidemic Diarrhea Virus Spike Recombinant Protein

García-Cambrón,

Cerriteño-Sánchez,

Lara-Romero

et al. 2024

Viruses

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Porcine epidemic diarrhea virus (PEDV) has affected the pork industry worldwide and during outbreaks the mortality of piglets has reached 100%. Lipid nanocarriers are commonly used in the development of immunostimulatory particles due to their biocompatibility and slow-release delivery properties. In this study, we developed a lipid nanoparticle (LNP) complex based on glycyrrhizinic acid (GA) and tested its efficacy as an adjuvant in mice immunized with the recombinant N-terminal domain (NTD) of porcine epidemic diarrhea virus (PEDV) spike (S) protein (rNTD-S). The dispersion stability analysis (Z-potential −27.6 mV) confirmed the size and charge stability of the LNP-GA, demonstrating that the particles were homogeneously dispersed and strongly anionic, which favors nanoparticles binding with the rNTD-S protein, which showed a slightly positive charge (2.11 mV) by in silico analysis. TEM image of LNP-GA revealed nanostructures with a spherical-bilayer lipid vesicle (~100 nm). The immunogenicity of the LNP-GA-rNTD-S complex induced an efficient humoral response 14 days after the first immunization (p < 0.05) as well as an influence on the cellular immune response by decreasing serum TNF-α and IL-1β concentrations, which was associated with an anti-inflammatory effect.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Glycyrrhizinic Acid-Based Lipid Nanoparticle (LNP-GA) as An Adjuvant That Improves the Immune Response to Porcine Epidemic Diarrhea Virus Spike Recombinant Protein

García-Cambrón,

Cerriteño-Sánchez,

Lara-Romero

et al. 2024

Viruses

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“…The common linkers include amide, carbamate, disulfide, ester, ether, ketal, phosphate, etc. The polycarbon hydrophobic tails play an important role in the formation of nanostructures and their efficiency (Viegas et al., 2022 ). The long tail groups determine the fluidity, dissociative nature in acid/basic medium, and lipophilicity, including fusion probability.…”

Section: Er Targeting Via Lipophilic Membranementioning

confidence: 99%

Intricate subcellular journey of nanoparticles to the enigmatic domains of endoplasmic reticulum

Girigoswami,

Pallavi,

Girigoswami

2023

Drug Delivery

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It is evident that site-specific systemic drug delivery can reduce side effects, systemic toxicity, and minimal dosage requirements predominantly by delivering drugs to particular pathological sites, cells, and even subcellular structures. The endoplasmic reticulum (ER) and associated cell organelles play a vital role in several essential cellular functions and activities, such as the synthesis of lipids, steroids, membrane-associated proteins along with intracellular transport, signaling of Ca 2+ , and specific response to stress. Therefore, the dysfunction of ER is correlated with numerous diseases where cancer, neurodegenerative disorders, diabetes mellitus, hepatic disorder, etc., are very common. To achieve satisfactory therapeutic results in certain diseases, it is essential to engineer delivery systems that can effectively enter the cells and target ER. Nanoparticles are highly biocompatible, contain a variety of cargos or payloads, and can be modified in a pliable manner to achieve therapeutic effectiveness at the subcellular level when delivered to specific organelles. Passive targeting drug delivery vehicles, or active targeting drug delivery systems, reduce the nonselective accumulation of drugs while reducing side effects by modifying them with small molecular compounds, antibodies, polypeptides, or isolated bio-membranes. The targeting of ER and closely associated organelles in cells using nanoparticles, however, is still unsymmetrically understood. Therefore, here we summarized the pathophysiological prospect of ER stress, involvement of ER and mitochondrial response, disease related to ER dysfunctions, essential therapeutics, and nanoenabled modulation of their delivery to optimize therapy.

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“…Consequently, due to these limitations and other disadvantages, liposomes are not considered robust enough for drug delivery. So, these issues led to the development of other lipid-based nanocarriers [ 144 , 145 ].…”

Section: Nano-based Drug Delivery Systems For Pancreatic Cancer Treat...mentioning

confidence: 99%

Advances in Pancreatic Cancer Treatment by Nano-Based Drug Delivery Systems

Viegas,

Patrício,

Prata

et al. 2023

Pharmaceutics

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Pancreatic cancer represents one of the most lethal cancer types worldwide, with a 5-year survival rate of less than 5%. Due to the inability to diagnose it promptly and the lack of efficacy of existing treatments, research and development of innovative therapies and new diagnostics are crucial to increase the survival rate and decrease mortality. Nanomedicine has been gaining importance as an innovative approach for drug delivery and diagnosis, opening new horizons through the implementation of smart nanocarrier systems, which can deliver drugs to the specific tissue or organ at an optimal concentration, enhancing treatment efficacy and reducing systemic toxicity. Varied materials such as lipids, polymers, and inorganic materials have been used to obtain nanoparticles and develop innovative drug delivery systems for pancreatic cancer treatment. In this review, it is discussed the main scientific advances in pancreatic cancer treatment by nano-based drug delivery systems. The advantages and disadvantages of such delivery systems in pancreatic cancer treatment are also addressed. More importantly, the different types of nanocarriers and therapeutic strategies developed so far are scrutinized.

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An insight on lipid nanoparticles for therapeutic proteins delivery

Cited by 25 publications

References 105 publications

Development of Glycyrrhizinic Acid-Based Lipid Nanoparticle (LNP-GA) as An Adjuvant That Improves the Immune Response to Porcine Epidemic Diarrhea Virus Spike Recombinant Protein

Development of Glycyrrhizinic Acid-Based Lipid Nanoparticle (LNP-GA) as An Adjuvant That Improves the Immune Response to Porcine Epidemic Diarrhea Virus Spike Recombinant Protein

Intricate subcellular journey of nanoparticles to the enigmatic domains of endoplasmic reticulum

Advances in Pancreatic Cancer Treatment by Nano-Based Drug Delivery Systems

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