An integratable microfluidic cartridge for forensic swab samples lysis

Yang, Jianing; Brooks, Carla; Estes, Matthew D.; Hurth, Cédric; Zenhausern, Frédéric

doi:10.1016/j.fsigen.2013.08.012

Cited by 14 publications

(15 citation statements)

References 22 publications

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“…DNA biochips enable the miniaturization, integration, and automation of tests, and can perform thousands of biological reactions in a few seconds [113][114][115]. Integrated and mobile rapid-DNA devices, which are designed for several purposes, can produce quality STR-profiles suitable for reference or as database samples [80,116].…”

Section: Extraction Of Compound And/or Challenging Samplesmentioning

confidence: 99%

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The importance of biological traces and evidences related to a criminal matter has been recognized for a long time. The examination of the expression of genetic polymorphism has been an integral part of the multidisciplinary field of medico-legal autopsy for over a century. Since the initial application of blood group antigens for personalization of a putative perpetrator in a murder case, the discipline of forensic genetics has evolved as a standard of forensic sciences. The real breakthrough, the application of molecular tools and processes for the in-vitro replication of genetic substances, has increasingly allowed the exploitation of advances of molecular genetics for both forensic and criminal investigations. Although there are certainly many more applications and scientific fields in the medico-legal arena, the relatively fast progress of genetics, which has accelerated recently with state-of-art technologies, can provide ever more relevant information in relation to a corpse or the cause and manner that resulted in the corpse for autopsy. This topic concerns the currently accepted forensic DNA technology, and the last section reviews commonly used markers for nuclear and mitochondrial DNA analysis as well as ongoing research. This review also focuses on the increasingly important non-human sources of DNA, and shortly covers the main aspects of animal forensic DNA examination.

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Section: Extraction Of Compound And/or Challenging Samplesmentioning

confidence: 99%

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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“…Matrices composed of polydimethylacrylamide are also employed for a variety of applications outside of forensics. Sizing DNA with new matrices [ 57 ] and microfluidic platforms developed for STR analysis [ 58 , 59 ] are often compared to bench-top analyses achieved using polydimethylacrylamide matrices. Applications of polydimethylacrylamide matrices outside of forensics include multi-locus variable number repeat analysis to genotype several bacteria including Shigella spp.…”

Section: Separationmentioning

confidence: 99%

Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009–2014)

2015

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This review of capillary electrophoresis methods for DNA analyses covers critical advances from 2009 to 2014, referencing 184 citations. Separation mechanisms based on free-zone capillary electrophoresis, Ogston sieving, and reptation are described. Two prevalent gel matrices for gel-facilitated sieving, which are linear polyacrylamide and polydimethylacrylamide, are compared in terms of performance, cost, viscosity, and passivation of electroosmotic flow. The role of capillary electrophoresis in the discovery, design, and characterization of DNA aptamers for molecular recognition is discussed. Expanding and emerging techniques in the field are also highlighted.

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“…The principle of the DNA extraction is based on the DNA absorption on the silica surface in the presence of chaotropic salts such as guanidine HCl, the removal of residual protein contaminants with ethanol, and the release of the purified DNA . Thus, a microchip‐based DNA purification is generally conducted in the microchamber, which is packed with silica‐based materials such as silica beads, silica‐coated magnetic beads, or silica sol–gel . Due to the formation of the microsized pore structure, high surface‐to‐volume ratio is generated that can improve the DNA extraction efficiency.…”

Section: Microchip‐based Dna Purificationmentioning

confidence: 99%

“…Yang et al. presented a microfluidic cartridge to be applied for the swab‐in sample lysis module . Three different types (buccal, saliva, and blood) of swab samples were used for DNA extraction, and the extraction yield was compatible with that of the commercial kits.…”

Section: Microchip‐based Dna Purificationmentioning

confidence: 99%

Microchip‐based forensic short tandem repeat genotyping

Kim

Heo

et al. 2015

Electrophoresis

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Micro total analysis system (μTAS) or lab-on-a-chip (LOC) technology has advanced over decades, and the high performance for chemical and biological analysis has been well demonstrated with advantages of low sample consumption, rapid analysis time, high-throughput screening, and portability. In particular, μTAS or LOC based genetic applications have been extensively explored, and the short tandem repeat (STR) typing on a chip has garnered attention in the forensic community due to its special use for human identification in the field of mass disaster and missing person investigation, paternity testing, and perpetrator identification. The STR typing process consists of sample collection, DNA extraction, DNA quantitation, STR loci amplification, capillary electrophoretic separation, and STR profiling. Recent progress of microtechnology shows its ability to substitute the conventional analytical tools, and furthermore demonstrates total integration of the whole STR processes on a single wafer for on-site STR typing. In this review article, we highlighted some representative results for fluorescence labeling techniques, microchip-based DNA purification, on-chip polymerase chain reaction (PCR), a capillary electrophoretic microdevice, and a fully integrated microdevice for STR typing.

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An integratable microfluidic cartridge for forensic swab samples lysis

Cited by 14 publications

References 22 publications

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009–2014)

Microchip‐based forensic short tandem repeat genotyping

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