An integrated approach to the recovery of travertine biodegradation by combining phyto-cleaning with genomic characterization

Genova, Chiara; Grottoli, Alessandro; Zoppis, Emma; Cencetti, Claudia; Matricardi, Pietro; Favero, Gabriele

doi:10.1016/j.microc.2020.104918

Cited by 12 publications

(15 citation statements)

References 37 publications

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“…The success of these products is attributable to some of their intrinsic characteristics, in particular, (i) the atoxicity in their chemical composition and their being harmless towards the environment and human beings; (ii) their ability to incorporate organic solvents; and (iii) the ease of their application and removal procedures [ 29 , 38 , 39 ]. However, in the literature, there are only few papers investigating the effectiveness of PVA-hydrogels for cleaning rocks [ 5 , 40 , 41 , 42 , 43 ]. Recently, Riedo et al [ 44 ] tested the effectiveness of PVA-borax hydrogel in generic limestone samples treated with Paraloid B72 and cleaned using a 4-min gel application time.…”

Section: Discussionmentioning

confidence: 99%

Single-Sided Portable NMR Investigation to Assess and Monitor Cleaning Action of PVA-Borax Hydrogel in Travertine and Lecce Stone

et al. 2021

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In this work, we investigated the potential of PVA-borax hydrogel for cleaning limestones and the dependence of the cleaning on the porosity of the rock and on the action time of the hydrogel treatment. Towards this goal, we used a nuclear magnetic resonance (NMR) spectrometer, developed for non-invasive and non-destructive applications on cultural heritage. T2-NMR parameters were quantified on different samples of Lecce stone and Travertine cut perpendicular (Pe) and parallel (Pa) to the bedding planes under different experimental conditions: untreated samples, treated with Paraloid B72 and cleaned with PVA-PEO-borax hydrogel applied for 4 min and 2 h. The T2 results suggest that the effectiveness of the cleaning strongly depended on the porosity of the stones. In Lecce stone, the hydrogel seemed to eliminate both the paramagnetic impurities (in equal measure with 4 min and 2 h treatment) and Paraloid B72. In Travertine Pe, characterized by a smaller pore size compared to Lecce stone, no significant effects were found regarding both the cleaning and the treatment with Paraloid B72. In Travertine Pa, characterized by a larger pore size than the other two samples, the hydrogel seemed to clean the paramagnetic agents (it worked better if applied for a longer time) but it did not appear to have any effect on Paraloid B72 removal.

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Section: Discussionmentioning

confidence: 99%

Single-Sided Portable NMR Investigation to Assess and Monitor Cleaning Action of PVA-Borax Hydrogel in Travertine and Lecce Stone

et al. 2021

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“…However, inorganic materials have also been treated with these natural compounds [ 83 ]. Essential oils from Thymus vulgaris , Origanum vulgare , and Calamintha nepeta and their major components (thymol, carvacrol, pulegone) have been prepared within a hydrogel matrix (made of Gelrite, poly-vinylacetate, CaCl 2 , and Acemoll CC) to remove biofilm from a travertine wall of the Sapienza University in Rome [ 84 ]. Volatile extracts from Illicium verum , the flower buds of Syzygium armoaticum , Quercus infectoria , Coptis chinensis , and Phellodendron amurense were profitably tested with fungi isolated from biofilm on the World Heritage Yeongneung stone monument [ 82 ].…”

Section: Biological Methodsmentioning

confidence: 99%

The Control of Cultural Heritage Microbial Deterioration

2020

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The microbial deterioration of cultural heritage includes physical and chemical damage as well as aesthetic alteration. With the technological advancement, a plethora of techniques for removing unwanted microorganisms have opened up new opportunities for microbiologists and conservators. This article reviews the most applied, up-to-date, and sustainable techniques developed for the control of cultural heritage microbial deterioration presenting noteworthy case studies. These techniques include chemical methods, i.e., traditional biocides and nanoparticles; physical methods, such as mechanical removal, UV irradiation, gamma radiation, laser cleaning, heat shocking, microwaves, and dry ice treatment; and biological methods, such as natural molecules with biocidal activity, enzymes, and microorganisms. The application of control systems requires the comprehension of their behavior toward the unwanted microorganisms and possible interactions with the heritage materials. This overview shows also the control methods drawbacks for the purpose of creating awareness in selecting the most suitable technique or combination of techniques.

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“…Created with BioRender.com. 10.3389/fmicb.2022.1006946 Frontiers in Microbiology 05 frontiersin.org and collaborators (Genova et al, 2020), while other authors use sterile spatula for the removal of the first layer and collection of a section underneath (Ding et al, 2021). Minimally invasive methods are also important when considering other surfaces of biological origin as skeletal remains.…”

Section: Sample Preparationmentioning

confidence: 99%

“…On some occasions, low biomass recovery is imposed by the intrinsic limitations of the studied surface, namely when approaching heritage artifacts or monuments under special protection for which a maximal sampling quantity might be allowed. Recovery methods include sterile-swabbing (non-invasive nature) and use of tape (of a micro-invasive nature) according to the recent methodological development by Genova and collaborators ( Genova et al, 2020 ), while other authors use sterile spatula for the removal of the first layer and collection of a section underneath ( Ding et al, 2021 ). Minimally invasive methods are also important when considering other surfaces of biological origin as skeletal remains.…”

Section: Generation and Analysis Of Genomics Data For The Study Of Mi...mentioning

confidence: 99%

Advances in experimental and computational methodologies for the study of microbial-surface interactions at different omics levels

et al. 2022

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The study of the biological response of microbial cells interacting with natural and synthetic interfaces has acquired a new dimension with the development and constant progress of advanced omics technologies. New methods allow the isolation and analysis of nucleic acids, proteins and metabolites from complex samples, of interest in diverse research areas, such as materials sciences, biomedical sciences, forensic sciences, biotechnology and archeology, among others. The study of the bacterial recognition and response to surface contact or the diagnosis and evolution of ancient pathogens contained in archeological tissues require, in many cases, the availability of specialized methods and tools. The current review describes advances in in vitro and in silico approaches to tackle existing challenges (e.g., low-quality sample, low amount, presence of inhibitors, chelators, etc.) in the isolation of high-quality samples and in the analysis of microbial cells at genomic, transcriptomic, proteomic and metabolomic levels, when present in complex interfaces. From the experimental point of view, tailored manual and automatized methodologies, commercial and in-house developed protocols, are described. The computational level focuses on the discussion of novel tools and approaches designed to solve associated issues, such as sample contamination, low quality reads, low coverage, etc. Finally, approaches to obtain a systems level understanding of these complex interactions by integrating multi omics datasets are presented.

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An integrated approach to the recovery of travertine biodegradation by combining phyto-cleaning with genomic characterization

Cited by 12 publications

References 37 publications

Single-Sided Portable NMR Investigation to Assess and Monitor Cleaning Action of PVA-Borax Hydrogel in Travertine and Lecce Stone

Single-Sided Portable NMR Investigation to Assess and Monitor Cleaning Action of PVA-Borax Hydrogel in Travertine and Lecce Stone

The Control of Cultural Heritage Microbial Deterioration

Advances in experimental and computational methodologies for the study of microbial-surface interactions at different omics levels

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