An integrated bioprocess for fermentative production of protopanaxadiol by recycling ethanol waste during down-stream extraction process

Zhao, Fanglong; Zhang, Chuanbo; Nan, Weihua; Lu, Wenyu

doi:10.1016/j.jclepro.2019.06.190

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…The metabolite profile of ZSC-L. indicates the presence of 11 flavonoids tentatively identified as quercetin 3,7,3 -trimethyl ether (4), eriodictyol-7-O-glucoside (7), apigenin-7-O-glucoside (8), okanin-4 -O-glucoside (15), quercitrin (17), isoquercetin (19), kaempferol-3-O-α-L-arabinoside (29), luteolin 7,3 -diglucoside (30), isoorientin 3 ,6 -di-O-glucoside (34), quercetin 3-O-robinobioside (35), rutin (36). Among these flavonoids, (17), (19), (34), (35) and (36) were previously isolated from ZSC-L [10,34] (12), ceanothic acid (13), zizyberanalic acid (14), betulinic acid (16), lupeol acetate (20), betulin (23), and zizyberenalic acid (26). Additionally, fatty acid compounds identified and characterized as hydroxy-oleic acid (5), 23-methyl-5Z,9Z-tetracosadienoic acid (11), trihydroxy-oleic acid (25), n-hexadecanoic acid (27), hexadecanoic acid, ethyl ester (28), docosanedioic acid (31), octadecanoic acid, ethyl ester (33) , respectively.…”

Section: Hplc-esi-ms/ms Analysis Of Different Extracts Of Zizyphus Sp...mentioning

confidence: 99%

“…Solvent recovery and re-use in bioprocesses have the potential to decrease the pollution and waste formation substantially. However, the solvent recovery technique is typically energy-intensive and expensive [ 12 ]. In this research, an integrated bioprocess was designed for the fermentative synthesis of protopanaxadiol (PPD) from ethanol waste recycled in the downstream extraction process, which was resulted in solvent recovery and reutilization at a low cost [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…acetate: formic acid (T:E:F, 7:5:1; v/v/v) 0.47 in ethyl acetate-n-hexane (1:2) & 0.81 in toluene: ethyl acetate: formic acid (T:E:F, 7:5:1; v/v/v) Betulinic acid(16): IR (neat, cm −1 ) 3464 (br),2943, 2870, 1643, 1685, 1188, 883, 1454. MS: m/z M +1 : 457.3305, 407(22), 353(15), 339(12), 325(100), 240(13) 1. H NMR (CDCl 3 , 500 MHz): δ (ppm): 4.76 (1H, br s, H-29a), 4.63 (1H, br s, H-29b), 3.2 (1H, m, H-3), 1.7 (3H, s, H-30) 0.99 (3H, s), 0.98 (3H, s), 0.95 (3H, s), 0.84 (3H, s) and 0.77 (3H, s) 13.…”

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confidence: 99%

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Isolation, Structure Elucidation and Antimicrobial Evaluation of Natural Pentacyclic Triterpenoids and Phytochemical Investigation of Different Fractions of Ziziphus spina-christi (L.) Stem Bark Using LCHRMS Analysis

et al. 2022

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Ziziphus spina-christi L. (ZSC-L) is a tree with thorny branches, belongs to the family Rhamnaceae and grows in the sub-tropics. The purpose of this research is to isolate and partially purify bioactive components from the crude ethanol extract of the stem bark of ZSC-L. Besides, bioassay-guided fractionation of ZSC-L stem bark was conducted using different solvents. The solvents were reutilized to minimize the production cost and environmental harm. In addition, the antimicrobial activities of the fractions were analyzed, followed by metabolic profiling using LC-HRMS. The n-butanol fraction showed the highest antimicrobial efficacy, so it was subjected to further purification. For the first time, two major compounds were isolated from the stem bark of ZSC-L and identified as lupane-type pentacyclic triterpenoids betulinic acid and betulin. Both compounds were used as antibacterial and anticancer agents and considered as a green product as the extraction procedure reduced the use of hazardous chemicals. Metabolic characterization of ZSC-L and its bioactive fractions were performed using LC-HR-ESI-MS and the results revealed the dereplication of 36 compounds belonging to different chemical classes. Flavonoids and triterpenes were the most prominent metabolite classes in the different fractions. The molecular docking results were obtained by studying the interaction of betulin and betulinic acid with antimicrobial receptors (4UYM, 1IYL, 1AJ2, 6J7L, 1AD4, 2VEG) to support the in vitro results. Our study highlights that Ziziphus spina-christi and its phytoconstituents, especially triterpenoids, act as a promising antimicrobial candidate in pharmaceutical and clinical applications.

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