An Integrated Handheld Biosensing Device with IoT System for In-Situ and Rapid Detection of Multi-Mycotoxins

Jusoh, Noor Sheryna; Said, Nur Azura Mohd; Salam, Faridah; Awaludin, Norhafniza; Kadir, Adlin Azlina Abdul; Ismail, Mohammad Rejab

doi:10.1109/icssa54161.2022.9870938

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Cited by 2 publications

References 29 publications

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Recent trends in the multi-target electrochemical rapid detection of co-contaminated mycotoxins in foodstuffs

Veenuttranon,

Lu,

Geng

et al. 2025

TrAC Trends in Analytical Chemistry

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Recent trends in the multi-target electrochemical rapid detection of co-contaminated mycotoxins in foodstuffs

Veenuttranon,

Lu,

Geng

et al. 2025

TrAC Trends in Analytical Chemistry

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Immunosensing of Aflatoxin B1 and Ochratoxin A on a Portable Device as Point-of-Care

Said¹,

Jusoh²,

Awaludin³

et al. 2023

Infectious Diseases

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Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are potent mycotoxins produced by the fungal genus Aspergillus. Their occurrence in grain corn is alarming hence the need for rapid on-site detection. An immuno-based biosensor technique for detection of the aforementioned toxins is described here. Highly specific in-house polyclonal antibodies against AFB1 and OTA were employed as bioreceptors in a label-free electrochemical biosensor; immobilized on modified screen-printed carbon electrodes (SPCEs). The immuno-functionalized SPCEs were first characterized on a laboratory electrochemical workstation for proof-on-concept study using differential pulse voltammetry (DPV) electrochemical technique. An Android-based device is improvised as a portable electrochemical reader integrated with internet of thing (IoT) features which include cloud server and a dedicated website. Sensitivity achieved by the modified SPCEs on the portable device is superior compared to enzyme-linked immunosorbent assay (ELISA) method and lab-based electrochemical workstation. The miniaturized biosensor system has been successfully tested on cornfield for in-situ mycotoxins detection with simple sample extraction. Analysis performed on twenty samples were validated using chromatographic analysis. This biosensor-IoT system offers a potential application for real-time detection and the portable reader serves as an excellent tool for point-of-care in routine monitoring of harmful mycotoxins.

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An Integrated Handheld Biosensing Device with IoT System for In-Situ and Rapid Detection of Multi-Mycotoxins

Cited by 2 publications

References 29 publications

Recent trends in the multi-target electrochemical rapid detection of co-contaminated mycotoxins in foodstuffs

Recent trends in the multi-target electrochemical rapid detection of co-contaminated mycotoxins in foodstuffs

Immunosensing of Aflatoxin B1 and Ochratoxin A on a Portable Device as Point-of-Care

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