An integrated metabolomic strategy for the characterization of the effects of Chinese yam and its three active components on septic cardiomyopathy

Zhou, Ning; Zeng, Mengnan; Li, Kai; Yang, Yaxiong; Bai, Zhiyao; Zheng, Xiaoke; Feng, Weisheng

doi:10.1039/c8fo00688a

Cited by 18 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tryptophan is metabolized mainly by a rate-limiting enzyme, indoleamine 2,3-dioxygenase (IDO) through the kynurenine pathway (KP) ( Badawy, 2017 ). IDO can be driven by the gut microbiota and proinflammatory cytokines (e.g., interferon-γ, TNF-α, prostaglandin-E2 ( Li et al, 2020 ; Liu et al, 2021 ; Zhou, N. et al, 2018 ). In the present study, levels of tryptophan catabolites through the KP (e.g., kynurenic acid, xanthurenic acid) were increased in the model group, suggesting upregulated IDO activity, microbial dysbiosis and release of proinflammatory factors in PE.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of Descurainia sophia seeds extract and its fractions on pulmonary edema by untargeted urine and serum metabolomics strategy

et al. 2023

Self Cite

View full text Add to dashboard Cite

Background:Descurainia sophia seeds (DS) is a herbal medicine in traditional Chinese medicine (TCM) for treating lung diseases. We aimed to evaluate the therapeutic effect of DS and five of its fractions upon pulmonary edema (PE) through metabolomics analysis (MA) of urine and serum samples of rats.Methods: A PE model was established by intrathoracic injection of carrageenan. Rats were pretreated with DS extract or its five fractions (polysaccharides (DS-Pol); oligosaccharides (DS-Oli); flavonoid glycosides (DS-FG); flavonoid aglycone (DS-FA); fat oil fraction (DS-FO)) for seven consecutive days. Forty-eight hours after carrageenan injection, lung tissues were subjected to histopathology. MA of urine and serum was done by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry, respectively. Principal component analysis and orthogonal partial least squares-discriminant analysis were operated for the MA of rats and potential biomarkers related to treatment. Heatmaps and metabolic networks were constructed to explore how DS and its five fractions act against PE.Results: DS and its five fractions could all attenuate pathologic lung injury to different degrees, and DS-Oli, DS-FG, and DS-FO had a more potent effect compared with DS-Pol and DS-FA. DS-Oli, DS-FG, DS-FA, and DS-FO could regulate the metabolic profiles of PE rats, but DS-Pol was less potent. According to MA, the five fractions could improve PE to some degree due to their anti-inflammatory, immunoregulatory, and renoprotective activities by mediating the metabolism of taurine, tryptophan, and arachidonic acid. However, DS-Oli, DS-FG, and DS-FO had more important roles in edema-fluid reabsorption, and reduction of vascular leakage through regulating the metabolism of phenylalanine, sphingolipid and bile acid. Finally, heatmaps and hierarchical clustering analysis indicated DS-Oli, DS-FG, and DS-FO to be more efficacious than DS-Pol or DS-FA against PE. The five fractions of DS had a synergistic effect on PE from different aspects, thereby constituting the entire efficacy of DS. DS-Oli, DS-FG, or DS-FO could be used as an alternative to DS.Conclusion: MA combined with use of DS and its fractions provided novel insights into the mechanism of action of TCM.

show abstract

Section: Discussionmentioning

confidence: 99%

Protective effects of Descurainia sophia seeds extract and its fractions on pulmonary edema by untargeted urine and serum metabolomics strategy

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has been reported that continuous infusion of TNF-α interferes with the synthesis of cardiac proteins and eventually reduces the synthesis of myocardial fiber and sarcoplasmic proteins, which may be related to the effects of TNF-α on mRNA translation efficiency (28). In addition, an increasing number of studies have shown that pro-inflammatory factors and anti-inflammatory factors are involved in the progression of sepsis (29)(30)(31). When the dynamic balance of pro-inflammatory and anti-inflammatory reactions is disrupted, the body becomes injured (32).…”

Section: Discussionmentioning

confidence: 99%

Parecoxib attenuates inflammation injury in septic H9c2 cells by regulating the MAPK signaling pathway

et al. 2023

View full text Add to dashboard Cite

Parecoxib, a non-steroidal anti-inflammatory drug, has been reported to possess protective effects against sepsis. However, its detailed role and underlying mechanisms in septic cardiomyopathy remain unclear. Therefore, the goal of the present study was to clarify the function and to investigate the mechanisms of parecoxib in lipopolysaccharide (LPS)-treated H9c2 rat cardiomyocytes. TNF-α, IL-1β and IL-6 expression levels in parecoxib-treated H9c2 cells stimulated with LPS were assessed using ELISA. Parecoxib-treated H9c2 cells stimulated with LPS were tested for viability using the Cell Counting Kit-8 assay. Western blotting analysis and 5-ethynyl-2'-deoxyuridine were used to evaluate cell proliferation. Apoptosis was assessed using TUNEL and western blotting. To assess the protein expression of the MAPK signaling pathway, western blotting was performed. The data showed that parecoxib significantly and dose-dependently reduced the inflammatory responses of LPS-treated H9c2 cells. Parecoxib also significantly and dose-dependently increased the proliferation and inhibited the apoptosis of LPS-treated H9c2 cells. In addition, parecoxib significantly suppressed the activation of the MAPK (p38, JNK and ERK) signaling pathway. The current study indicated that parecoxib could be a viable therapeutic option for septic cardiomyopathy.

show abstract

“…However, it is still a major challenge to alleviate sepsis-induced myocardial injury. Moreover, some recent reports have shown that herbal medicines could improve cardiac function and reduce myocardial enzyme levels in septic cardiomyopathy [ 6 , 7 ]. Therefore, it urgently needs more effective drugs for protection against myocardial injury in septic cardiomyopathy.…”

Section: Introductionmentioning

confidence: 99%

Allicin protects against LPS-induced cardiomyocyte injury by activating Nrf2-HO-1 and inhibiting NLRP3 pathways

Sun,

Xu,

Zhou

et al. 2023

BMC Cardiovasc Disord

View full text Add to dashboard Cite

Background Allicin is a bioactive compound with potent antioxidative activity and plays a protective effect in myocardial damage and fibrosis. The role and mechanism of Allicin in septic cardiomyopathy are unclear. In this study, we investigated the effects and underlying mechanisms of Allicin on lipopolysaccharide (LPS) induced injury in H9c2 cardiomyocytes. Methods H9c2 cardiomyocyte cells were pretreated with Allicin (0, 25, 50, and 100 µM) for 2 h, followed by incubation with LPS (10 µg/mL) for 24 h at 37 °C. Cell viability (cell counting kit-8 [CCK-8]), apoptosis (TUNEL staining), oxidative stress (malondialdehyde [MDA] and superoxide dismutase [SOD]), and cytokines release (Interleukin beta [IL-β], Interleukin 6 [IL-6], and tumor necrosis factor-alpha [TNF-α]) were determined. The mRNA and protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and NLR family pyrin domain containing 3 (NLRP3) signaling pathway molecules were quantified by real-time quantitative PCR (RT-qPCR) and western blot, respectively. Results Allicin had no effect on H9c2 cell viability but attenuated LPS-induced injury, with increased cell viability, reduction in inflammatory cytokines release, apoptosis, reduced MDA, and increased SOD (P < 0.05). Additionally, Allicin increased Nrf2 and cellular HO-1 expressions in LPS-treated H9c2 cells. Moreover, Allicin modulated the NLRP3 inflammasome, increased the cleaved caspase-1 (p10) protein, and attenuated the LPS-induced increase in NLRP3, pro-IL-1β, and IL-1β proteins. Silencing of Nrf2 by siRNA (siNrf2) significantly attenuated Allicin-induced increase in cell viability and HO-1 and decrease in NLRP3 protein in LPS-stimulated H9c2 cells. Conclusions Allicin protects cardiomyocytes against LPS‑induced injury through activation of Nrf2/HO-1 and inhibition of NLRP3 signaling pathways.

show abstract

An integrated metabolomic strategy for the characterization of the effects of Chinese yam and its three active components on septic cardiomyopathy

Cited by 18 publications

References 33 publications

Protective effects of Descurainia sophia seeds extract and its fractions on pulmonary edema by untargeted urine and serum metabolomics strategy

Protective effects of Descurainia sophia seeds extract and its fractions on pulmonary edema by untargeted urine and serum metabolomics strategy

Parecoxib attenuates inflammation injury in septic H9c2 cells by regulating the MAPK signaling pathway

Allicin protects against LPS-induced cardiomyocyte injury by activating Nrf2-HO-1 and inhibiting NLRP3 pathways

Contact Info

Product

Resources

About