An integrated microfluidic chip for treatment and detection of microalgae cells

Wang, Junsheng; Wang, Ge; Chen, Mengmeng; Wang, Yanjuan; Ding, Gege; Zhang, Yichi; Kang, Yuejun; Pan, Xinxiang

doi:10.1016/j.algal.2019.101593

Cited by 14 publications

(9 citation statements)

References 44 publications

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“…In the fluorescence imaging mode, the wavelength of excitation light used was 488 nm, because the absorption peak of chlorophyll spectrum in microalgae cells is 488 nm and the emission peak is 685 nm. That is, the microalgae cells can emit CF signals of 685 nm under the excitation of 488 nm monochromatic light [ 44 ]. The CF signal was received by the CMOS imaging module.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous Detection of Viability and Concentration of Microalgae Cells Based on Chlorophyll Fluorescence and Bright Field Dual Imaging

Wang

et al. 2021

Micromachines

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Ship ballast water contains high concentration of plankton, bacteria, and other microorganisms. If the huge amount of ballast water is discharged without being inactivated, it will definitely spell disaster to the marine environment. Microalgae is the most common species exiting in ballast water, so the detection of the concentration and viability of microalgae is a very important issue. The traditional methods of detecting microalgae in ballast water were costly and need the help of bulky equipment. Herein, a novel method based on microalgae cell intracellular chlorophyll fluorescence (CF) imaging combines with cell bright field (BF) microscopy was proposed. The geometric features of microalgae cells were obtained by BF image, and the cell viability was obtained by CF image. The two images were fused through the classic image registration algorithm to achieve simultaneous detection of the viability and concentration of microalgae cells. Furthermore, a low-cost, miniaturized CF/BF microscopy imaging prototype system based on the above principles was designed. In order to verify the effectiveness of the proposed method, four typical microalgae in ballast water (Platymonas, Pyramimonas sp., Chrysophyta, and Prorocentrum lima) were selected as the samples. The experimental results show that the self-developed prototype can quickly and accurately determine the concentration and the viability of microalgae cells in ship ballast water based on the dual images of BF and CF, and the detection accuracy is equivalent to that of commercial microscope. It was the first time to simultaneously detect the viability and concentration of microalgae cells in ship ballast water using the method that combining the fluorescence and bright field images; moreover, a miniaturized microscopic imaging prototype was developed. Those findings expected to contribute to the microalgae detection and ship ballast water management.

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Section: Methodsmentioning

confidence: 99%

Simultaneous Detection of Viability and Concentration of Microalgae Cells Based on Chlorophyll Fluorescence and Bright Field Dual Imaging

Wang

et al. 2021

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“…NaClO solution was used to inactivate microalgae. The concentration of NaClO solution was determined by an iodometric method as described previously [43].…”

Section: Methodsmentioning

confidence: 99%

A Microfluidic Prototype System towards Microalgae Cell Separation, Treatment and Viability Characterization

Wang

Zhou

et al. 2019

Sensors

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There are a huge number, and abundant types, of microalgae in the ocean; and most of them have various values in many fields, such as food, medicine, energy, feed, etc. Therefore, how to identify and separation of microalgae cells quickly and effectively is a prerequisite for the microalgae research and utilization. Herein, we propose a microfluidic system that comprised microalgae cell separation, treatment and viability characterization. Specifically, the microfluidic separation function is based on the principle of deterministic lateral displacement (DLD), which can separate various microalgae species rapidly by their different sizes. Moreover, a concentration gradient generator is designed in this system to automatically produce gradient concentrations of chemical reagents to optimize the chemical treatment of samples. Finally, a single photon counter was used to evaluate the viability of treated microalgae based on laser-induced fluorescence from the intracellular chlorophyll of microalgae. To the best of our knowledge, this is the first laboratory prototype system combining DLD separation, concentration gradient generator and chlorophyll fluorescence detection technology for fast analysis and treatment of microalgae using marine samples. This study may inspire other novel applications of micro-analytical devices for utilization of microalgae resources, marine ecological environment protection and ship ballast water management.

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“…The chlorophyll fluorescence intensity is proportional to the chlorophyll content, which can be used as an index to evaluate cell activity, growth and photosynthesis effectiveness [95][96][97]. It can also be used to quantify the concentration of living cells in the sample solution, as well as the resistance of microalgae to chemical reagents [98]. As the pigments in different species have different proportions, this method can also be used to classify the microalgae while simultaneously taking into consideration the cell shape and size [78].…”

Section: Fluorescence-based Sensingmentioning

confidence: 99%

The Fusion of Microfluidics and Optics for On-Chip Detection and Characterization of Microalgae

Zheng

Duan

et al. 2021

Micromachines

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It has been demonstrated that microalgae play an important role in the food, agriculture and medicine industries. Additionally, the identification and counting of the microalgae are also a critical step in evaluating water quality, and some lipid-rich microalgae species even have the potential to be an alternative to fossil fuels. However, current technologies for the detection and analysis of microalgae are costly, labor-intensive, time-consuming and throughput limited. In the past few years, microfluidic chips integrating optical components have emerged as powerful tools that can be used for the analysis of microalgae with high specificity, sensitivity and throughput. In this paper, we review recent optofluidic lab-on-chip systems and techniques used for microalgal detection and characterization. We introduce three optofluidic technologies that are based on fluorescence, Raman spectroscopy and imaging-based flow cytometry, each of which can achieve the determination of cell viability, lipid content, metabolic heterogeneity and counting. We analyze and summarize the merits and drawbacks of these micro-systems and conclude the direction of the future development of the optofluidic platforms applied in microalgal research.

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An integrated microfluidic chip for treatment and detection of microalgae cells

Cited by 14 publications

References 44 publications

Simultaneous Detection of Viability and Concentration of Microalgae Cells Based on Chlorophyll Fluorescence and Bright Field Dual Imaging

Simultaneous Detection of Viability and Concentration of Microalgae Cells Based on Chlorophyll Fluorescence and Bright Field Dual Imaging

A Microfluidic Prototype System towards Microalgae Cell Separation, Treatment and Viability Characterization

The Fusion of Microfluidics and Optics for On-Chip Detection and Characterization of Microalgae

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