An integrated microfluidic/microelectrode array for the study of activity-dependent intracellular dynamics in neuronal networks

Moutaux, Eve; Charlot, B.; Genoux, Aurélie; Saudou, Frédéric; Cazorla, Maxime

doi:10.1039/c8lc00694f

Cited by 79 publications

(84 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Once a neural circuit is implemented on chip, the possibility to record the electrical activity of the neuronal populations in real time is an essential requirement to acquire a basic understanding of the network functionality. To this aim, several studies have successfully demonstrated the integration of commercial or custom-made multielectrode arrays (MEAs) within microfluidic chips [ 53 , 133 , 134 , 135 , 136 , 137 , 138 ]. In details, MEAs are composed of a set of microelectrodes typically arranged in a matrix configuration, that enable to record extracellular activity of neuronal populations from multiple sites simultaneously in a noninvasive way [ 139 , 140 , 141 ].…”

Section: Biosensors For Measuring Ooc’ Electrical Activitymentioning

confidence: 99%

“…For instance, Moutaux et al developed an on-chip platform that combines a compartmentalized microfluidic device with a dedicated MEA to selectively record electrical activity in presynaptic axonal projections and postsynaptic neuronal targets, while simultaneously studying intracellular dynamics via high-resolution videomicroscopy ( Figure 5 a) [ 136 ]. The microfluidic platform consists of two opposite neuronal chambers connected via an intermediate synaptic chamber through microchannels of different lengths, i.e., 500 µm for presynaptic neurons and 75 µm for postsynaptic neurons [ 132 ].…”

Section: Biosensors For Measuring Ooc’ Electrical Activitymentioning

confidence: 99%

See 1 more Smart Citation

Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

et al. 2020

View full text Add to dashboard Cite

Organs-on-chip (OoC), often referred to as microphysiological systems (MPS), are advanced in vitro tools able to replicate essential functions of human organs. Owing to their unprecedented ability to recapitulate key features of the native cellular environments, they represent promising tools for tissue engineering and drug screening applications. The achievement of proper functionalities within OoC is crucial; to this purpose, several parameters (e.g., chemical, physical) need to be assessed. Currently, most approaches rely on off-chip analysis and imaging techniques. However, the urgent demand for continuous, noninvasive, and real-time monitoring of tissue constructs requires the direct integration of biosensors. In this review, we focus on recent strategies to miniaturize and embed biosensing systems into organs-on-chip platforms. Biosensors for monitoring biological models with metabolic activities, models with tissue barrier functions, as well as models with electromechanical properties will be described and critically evaluated. In addition, multisensor integration within multiorgan platforms will be further reviewed and discussed.

show abstract

Section: Biosensors For Measuring Ooc’ Electrical Activitymentioning

confidence: 99%

Section: Biosensors For Measuring Ooc’ Electrical Activitymentioning

confidence: 99%

Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Because these are recorded noninvasively, repeated measurements can be achieved over time in either brain slices or tissue culture preparations. [259] Based on the design of the microfluidic system and MEA, research can range from investigating intracellular dynamics in a single axon [260] to propagation between neuronal sub-populations (e.g., cortical-thalmic) in a co-culture system. [261] Although not part of a microfluidic chip, MEAs have also shown utility in drug screening applications such as the evaluation of neurotoxic compounds.…”

Section: Technology and Systems Integration In Microfluidic Platformsmentioning

confidence: 99%

Innovations in 3D Tissue Models of Human Brain Physiology and Diseases

Lovett

Nieland

Dingle

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

3D laboratory tissue cultures have emerged as an alternative to traditional 2D culture systems that do not recapitulate native cell behavior. The discrepancy between in vivo and in vitro tissue-cell-molecular responses impedes understanding of human physiology in general and creates roadblocks for the discovery of therapeutic solutions. Two parallel approaches have emerged for the design of 3D culture systems. The first is biomedical engineering methodology, including bioengineered materials, bioprinting, microfluidics, and bioreactors, used alone or in combination, to mimic the microenvironments of native tissues. The second approach is organoid technology, in which stem cells are exposed to chemical and/or biological cues to activate differentiation programs that are reminiscent of human (prenatal) development. This review article describes recent technological advances in engineering 3D cultures that more closely resemble the human brain. The contributions of in vitro 3D tissue culture systems to new insights in neurophysiology, neurological diseases, and regenerative medicine are highlighted. Perspectives on designing improved tissue models of the human brain are offered, focusing on an integrative approach merging biomedical engineering tools with organoid biology.

show abstract

“…The device has three microfluidic chambers, presynaptic, synaptic, and postsynaptic chambers (each) with axonal, reference, and postsynaptic electrodes to record the activity of single projecting axon. These presynaptic axons were recorded selectively by placing electrodes under the presynaptic chamber, and this study was further extended to study calcium dynamics [196]. Figure 20 depicts a microfluidic DEP device consisted of a PDMS microfluidic chip with ring-shaped indium tin oxide microelectrode array.…”

Section: Micro/nanofluidic Devices For Single-neuron Analysismentioning

confidence: 99%

A Single-Neuron: Current Trends and Future Prospects

Gupta

Balasubramaniam

Chang

et al. 2020

Cells

View full text Add to dashboard Cite

The brain is an intricate network with complex organizational principles facilitating a concerted communication between single-neurons, distinct neuron populations, and remote brain areas. The communication, technically referred to as connectivity, between single-neurons, is the center of many investigations aimed at elucidating pathophysiology, anatomical differences, and structural and functional features. In comparison with bulk analysis, single-neuron analysis can provide precise information about neurons or even sub-neuron level electrophysiology, anatomical differences, pathophysiology, structural and functional features, in addition to their communications with other neurons, and can promote essential information to understand the brain and its activity. This review highlights various single-neuron models and their behaviors, followed by different analysis methods. Again, to elucidate cellular dynamics in terms of electrophysiology at the single-neuron level, we emphasize in detail the role of single-neuron mapping and electrophysiological recording. We also elaborate on the recent development of single-neuron isolation, manipulation, and therapeutic progress using advanced micro/nanofluidic devices, as well as microinjection, electroporation, microelectrode array, optical transfection, optogenetic techniques. Further, the development in the field of artificial intelligence in relation to single-neurons is highlighted. The review concludes with between limitations and future prospects of single-neuron analyses.

show abstract

An integrated microfluidic/microelectrode array for the study of activity-dependent intracellular dynamics in neuronal networks

Abstract: A microfluidics/MEA platform was developed to control neuronal activity while imaging intracellular dynamics within reconstituted neuronal networks.

Cited by 79 publications

References 47 publications

Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

Innovations in 3D Tissue Models of Human Brain Physiology and Diseases

A Single-Neuron: Current Trends and Future Prospects

Contact Info

Product

Resources

About