An Integrated Paper Microfluidic Device Based on Isothermal Amplification for Simple Sample-to-Answer Detection of Campylobacter jejuni

Abstract

The global health and economic burden of Campylobacter prompts the development of novel detection techniques that can be implemented in resource-limited and on-site settings. This study described point-of-need identification of C. jejuni using a hybrid paper/polymer-based microfluidic device that is easy to operate.

Categorising hybrid material microfluidic devices

“…Similarly to primary component hybrid devices, the majority of listed devices are comprised of polymer materials as a base substrate and are often combined with glass, but more recently a range of other materials such as paper, hydrogels and other inorganic materials have also been used. Hydrogels have been used as bioscaffolds (Trappmann et al, 2017) for cell attachment whereas paper has been used as a fluid carrier (Chen et al, 2023). The photoresist SU-8 has extremely high biocompatibility and has historically been utilised as a critical material in microelectronics (Nemani et al, 2013).…”

Ensuring food safety: Microfluidic‐based approaches for the detection of food contaminants

“…Furthermore, paper microfluidic devices developed within the past few years have demonstrated the detection of L. monocytogenes, Campylobacter jejuni, S. aureus and Vibrio parahaemolyticus . 67 , 68 , 69 …”

“…This chip displayed the detection of Salmonella typhimurium as low as 1.0 × 102 copies/µL within 30 min, offering significantly simplified and quicker on-site detection of Salmonella compared to PCR-based methods. Furthermore, paper microfluidic devices developed within the past few years have demonstrated the detection of L. monocytogenes, Campylobacter jejuni, S. aureus and Vibrio parahaemolyticus [67][68][69].…”