An integrated strategy for discovering effective components of Shaoyao Gancao decoction for treating neuropathic pain by the combination of partial least-squares regression and multi-index comprehensive method

Feng, Lin; Chen, Yanyan; Xu, Ding‐Qiao; Fu, Ruonong; Yue, Shi‐Jun; Zhao, Qi; Huang, Yu‐Ning; Bai, Xue; Wang, Mei; Xing, Li-Ming; Tang, Yuping; Duan, Jin‐Ao

doi:10.1016/j.jep.2020.113050

Cited by 19 publications

(10 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detailed information regarding the chemical compounds present in JGd was obtained from TCMSP [80] (Supplementary Table S1), and the active compounds were defined as those with OB ≥ 30%, Caco-2 permeability ≥ −0.4, and DL ≥ 0.18, as described previously [63,80,81]. Some components were also determined to be active because of the substantial amount contained in JGd and their reported relevant pharmacological activity [42,57,[114][115][116][117][118][119][120][121][122][123][124][125][126][127][128], although they did not meet the criteria. As a result, 111 active chemical compounds were obtained for JGd (Supplementary Table S2).…”

Section: Active Chemical Compounds Of Jakyak-gamchomentioning

confidence: 99%

“…e analgesic mechanisms of JGd include the modulation of spinal α2-adrenoceptors, transient receptor potential vanilloid 1 (TRPV1) channels, calcium and Sirt1 signalings, muscle contraction and relaxation, and chemokine and cytokine expression [39,44,46,51,[57][58][59][60]. However, the pharmacological properties of JGd at the systemic level need to be explored.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Investigation of the Molecular Mechanisms Underlying the Analgesic Effect of Jakyak‐Gamcho Decoction: A Network Pharmacology Study

Lee¹,

Kang

Park³

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Herbal drugs have drawn substantial interest as effective analgesic agents; however, their therapeutic mechanisms remain to be fully understood. To address this question, we performed a network pharmacology study to explore the system-level mechanisms that underlie the analgesic activity of Jakyak-Gamcho decoction (JGd; Shaoyao-Gancao-Tang in Chinese and Shakuyaku-Kanzo-To in Japanese), an herbal prescription consisting of Paeonia lactiflora Pallas and Glycyrrhiza uralensis Fischer. Based on comprehensive information regarding the pharmacological and chemical properties of the herbal constituents of JGd, we identified 57 active chemical compounds and their 70 pain-associated targets. The JGd targets were determined to be involved in the regulation of diverse biological activities as follows: calcium- and cytokine-mediated signalings, calcium ion concentration and homeostasis, cellular behaviors of muscle and neuronal cells, inflammatory response, and response to chemical, cytokine, drug, and oxidative stress. The targets were further enriched in various pain-associated signalings, including the PI3K-Akt, estrogen, ErbB, neurotrophin, neuroactive ligand-receptor interaction, HIF-1, serotonergic synapse, JAK-STAT, and cAMP pathways. Thus, these data provide a systematic basis to understand the molecular mechanisms underlying the analgesic activity of herbal drugs.

show abstract

Section: Active Chemical Compounds Of Jakyak-gamchomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Investigation of the Molecular Mechanisms Underlying the Analgesic Effect of Jakyak‐Gamcho Decoction: A Network Pharmacology Study

Lee¹,

Kang

Park³

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…In order to remove interfering substances in serum samples and enrich the targeted compounds for more sensitive detection, serum samples were generally pretreated by the methods of protein precipitation, liquid–liquid extraction and solid-phase extraction, respectively ( Feng et al, 2020 , Yan et al, 2019 , Zhang et al, 2019 ). Moreover, addition of a certain amount of acid, the performance of pretreatment can be improved for the acidic compounds.…”

Section: Resultsmentioning

confidence: 99%

Identification of prototype compounds and their metabolites in rats’ serum from Xuefu Zhuyu Decoction by UPLC-Q-TOF/MS

Zhang

Jiang²,

Zhang³

et al. 2023

Chinese Herbal Medicines

View full text Add to dashboard Cite

“…The Selection Of Q-markers In Zq 2.1. Quantitative identi cation of Q-markers for ZQ based on AHP-EWM In this step our study adopted the traditional Chinese medicine Q-markers quantitative identi cation system based on AHP-EWM established by Tang Yuping research group [10][11][12][13][14]. There, analytic hierarchy process (AHP) method was applied to the weight analysis of the validity, testability, and speci city of the rst-level (or called the factor layer) indexes, andentropy weight method (EWM) was used to analyze the second-level (or called the control layer) indexes supported by literature and experimental data.…”

Section: Introductionmentioning

confidence: 99%

Preparation and evaluation of Orange Fruit Reference Extract based on the principles of Q-markers-A general procedure for establishing Herbal Reference Extracts used for identification

Zhao

Wang

Guo³

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Herbal Reference Extracts (HREs) have been used for the overall quality control (QC) of Traditional Chinese Medicines (TCMs) and used as an effective strategy for solving the shortage of reference standards in quality evaluation of TCMs in recently years. But the preparation and evaluation of it has rarely been reported. The ideal HREs should be a combination of Chemical Reference (CR) and Efficacy Reference (ER). However, it is not realistic to achieve "completely extracted" of TCMs at the present stage. Quality markers (Q-markers) provides a series of effective and scientific strategies for QC of TCMs. And Q-markers-based HREs should be a meaningful attempt to promote the standardization of quality evaluation system for TCMs. Purpose Preparation adequate HREs based on the principles of Q-markers, and used for quality evaluation of relevant preparation products. Then propose the general procedure for establishing HREs used for identification. Methods The authenticity, specificity, consistency and stability (ASCS) were the four essential principles of HREs. In this study, we chose Aurantii Fructus as an example, Firstly, selected 7 Q-markers of it which has the properties of specificity, effectiveness and testability based on the combination of analytic hierarchy process and entropy weight method (AHP-EWM). Secondly, preparated its reference extract which is called Orange Fruit Reference Extract (OFRE) based on ASCS and its Q-markers. Thirdly, established the QC method of OFRE according to its usage requirements. And finally, demonstrated its application of the thin layer chromatography (TLC) identification and characteristic chromatogram identification in the Orange fruit formula granule (or called Zhiqiao Peifangkeli, ZQPK). Results Compared with the Herbal Reference Substance (HRS) which with characteristic appearance or morphological structure, OFRE can be used for TLC identification of ZQPK conveniently, without complicated extraction or purification steps. And in the analysis of characteristic chromatogram of ZQPK, the OFRE can provid a rather simple profile, the main naringin, neohesperidin, nobiletin and tangeretin were clearly separated, which indicated that the OFRE could be used to identify the characteristic chromatogram of ZQPK and reduce the use of Chemical Reference Substance (CRS). And finally, we propose the general procedure for establishing HREs used for identification. Conclusion In summary, Q-markers-based HREs is feasibility, and should have a broader application prospect. And this research concept can serve as a reference for study on preparation, evaluation and application of HREs used for identification.

show abstract

An integrated strategy for discovering effective components of Shaoyao Gancao decoction for treating neuropathic pain by the combination of partial least-squares regression and multi-index comprehensive method

Cited by 19 publications

References 33 publications

An Investigation of the Molecular Mechanisms Underlying the Analgesic Effect of Jakyak‐Gamcho Decoction: A Network Pharmacology Study

An Investigation of the Molecular Mechanisms Underlying the Analgesic Effect of Jakyak‐Gamcho Decoction: A Network Pharmacology Study

Identification of prototype compounds and their metabolites in rats’ serum from Xuefu Zhuyu Decoction by UPLC-Q-TOF/MS

Preparation and evaluation of Orange Fruit Reference Extract based on the principles of Q-markers-A general procedure for establishing Herbal Reference Extracts used for identification

Contact Info

Product

Resources

About