An integrated strategy toward comprehensive characterization and quantification of multiple components from herbal medicine: An application study in Gelsemium elegans

Zuo, Meng-Ting; Sun, Zhi-Liang; Lin, Li; Tang, Qi; Cheng, Pi; Liu, Zhaoying

doi:10.1016/j.chmed.2020.06.002

Cited by 19 publications

(7 citation statements)

References 41 publications

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“…The roots, stems and leaves of mature G. elegans were respectively divided into three parts according to the maturity degree, and the leaves were separated into mesophyll and vein. The sample preparation method was performed according to previously reported [ 54 ]. 100 mg sample was ground in a mortar with liquid nitrogen, and then extracted with 2.5 mL of 80% ethanol ( v / v ) in a 60 °C ultrasonic machine (Shumei KQ-250DE, Kunshan, China) for 30 min.…”

Section: Methodsmentioning

confidence: 99%

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In Situ Visual Distribution of Gelsemine, Koumine, and Gelsenicine by MSI in Gelsemiumelegans at Different Growth Stages

Luo

et al. 2022

Molecules

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The distribution of pharmatically important alkaloids gelsemine, koumine, and gelsenicine in Gelsemium elegans tissues is a hot topic attracting research attention. Regretfully, the in planta visual distribution details of these alkaloids are far from clear although several researches reported the alkaloid quantification in G. elegans by LC-MS/MS. In this study, mass imaging spectrometry (MSI) was employed to visualize the in situ visualization of gelsemine, koumine, and gelsenicine in different organs and tissues of G. elegans at different growth stages, and the relative quantification of three alkaloids were performed according to the image brightness intensities captured by the desorption electrospray ionization MSI (DESI-MSI). The results indicated that these alkaloids were mainly accumulated in pith region and gradually decreased from pith to epidermis. Interestingly, three alkaloids were found to be present in higher abundance in the leaf vein. Along with the growth and development, the accumulation of these alkaloids was gradually increased in root and stem. Moreover, we employed LC-MS/MS to quantify three alkaloids and further validated the in situ distributions. The content of koumine reached 249.2 μg/g in mature roots, 272.0 μg/g in mature leaves, and 149.1 μg/g in mature stems, respectively, which is significantly higher than that of gelsemine and gelsenicine in the same organ. This study provided an accurately in situ visualization of gelsemine, koumine, and gelsenicine in G. elegans, and would be helpful for understanding their accumulation in plant and guiding application.

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Section: Methodsmentioning

confidence: 99%

“…The analytical method was performed according to previous report [ 54 ]. Alkaloid quantification in G. elegans was performed on an Agilent series 1290 Infinity HPLC instrument coupled with an Agilent 6460 MS/MS (Agilent Technologies, Santa Clara, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

In Situ Visual Distribution of Gelsemine, Koumine, and Gelsenicine by MSI in Gelsemiumelegans at Different Growth Stages

Luo

et al. 2022

Molecules

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“…Existing research of litchi pericarp revealed the presence of polyphenols, mainly including (+)-catechin, rutin, (-)-epigallocatechin, 3,4-dihydroxybenzoic acid, quercetin, epicatechin, epigallocatechin gallate, procyanidin A2, procyanidin B2, procyanidin A1, and procyanidin B1. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying compounds in LPE was proposed [ 16 ]. We dissolved the LPE and reference materials in an aqueous solution of 50% methanol.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Chemical Constituents of Litchi Pericarp Extracts and Its Antioxidant Activity in Mice

Yang

Zhang

et al. 2022

Foods

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Litchi pericarp is the main byproduct of litchi processing and contains several polyphenols. However, the chemical constituents and the antioxidant effect in litchi pericarp extracts (LPE) have been rarely studied. The result of the quantitative analyses of the major monomers in LPE indicated that procyanidin A2, procyanidin B2, epicatechin, rutin, and catechin were the major polyphenol compounds of LPE. The LPE exhibited high radical scavenging activity, as indicated by the results of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ascorbic acid, 2,2′-Azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) tests. Moreover, administrating D-galactose in mice led to the reduced activity of antioxidant enzymes, aggravated lipid peroxidation, and induced protein oxidation. The results were improved in the aging mice after the LPE treatment was performed. The above results suggest that LPE has an excellent antioxidant effect. Accordingly, litchi pericarp can serve as a promising source of dietary antioxidants.

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“…On the other hand, an untargeted metabolomics platform can offer useful insights into the evaluation of the quality of traditional Chinese medicines, including easily confusing species [ 35 , 36 ], species of different geographic origins [ 37 ], different parts of the same plant [ 38 ], and different growing ages amongst species [ 39 , 40 ]. Pseudo-targeted metabolomics has been proven to be feasible with a variety of advantages, including the improvement in the detection sensitivity for those low-abundance metabolites, which can thus expand the screening range of the potential biomarkers in metabolomics studies [ 41 , 42 , 43 ].…”

Section: Introductionmentioning

confidence: 99%

Integrating Enhanced Profiling and Chemometrics to Unveil the Potential Markers for Differentiating among the Leaves of Panax ginseng, P. quinquefolius, and P. notoginseng by Ultra-High Performance Liquid Chromatography/Ion Mobility-Quadrupole Time-of-Flight Mass Spectrometry

et al. 2022

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The leaves of Panax species (e.g., Panax ginseng-PGL, P. quinquefolius-PQL, and P. notoginseng-PNL) can serve as a source for healthcare products. Comprehensive characterization and unveiling of the metabolomic difference among PGL, PQL, and PNL are critical to ensure their correct use. For this purpose, enhanced profiling and chemometrics were integrated to probe into the ginsenoside markers for PGL/PQL/PNL by ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (UHPLC/IM-QTOF-MS). A hybrid scan approach (HDMSE-HDDDA) was established achieving the dimension-enhanced metabolic profiling, with 342 saponins identified or tentatively characterized from PGL/PQL/PNL. Multivariate statistical analysis (33 batches of leaf samples) could unveil 42 marker saponins, and the characteristic ginsenosides diagnostic for differentiating among PGL/PQL/PNL were primarily established. Compared with the single DDA or DIA, the HDMSE-HDDDA hybrid scan approach could balance between the metabolome coverage and spectral reliability, leading to high-definition MS spectra and the additional collision-cross section (CCS) useful to differentiate isomers.

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An integrated strategy toward comprehensive characterization and quantification of multiple components from herbal medicine: An application study in Gelsemium elegans

Cited by 19 publications

References 41 publications

In Situ Visual Distribution of Gelsemine, Koumine, and Gelsenicine by MSI in Gelsemiumelegans at Different Growth Stages

In Situ Visual Distribution of Gelsemine, Koumine, and Gelsenicine by MSI in Gelsemiumelegans at Different Growth Stages

Evaluation of Chemical Constituents of Litchi Pericarp Extracts and Its Antioxidant Activity in Mice

Integrating Enhanced Profiling and Chemometrics to Unveil the Potential Markers for Differentiating among the Leaves of Panax ginseng, P. quinquefolius, and P. notoginseng by Ultra-High Performance Liquid Chromatography/Ion Mobility-Quadrupole Time-of-Flight Mass Spectrometry

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