An integrated view on a eukaryotic osmoregulation system

Hohmann, Stefan

doi:10.1007/s00294-015-0475-0

Cited by 118 publications

(95 citation statements)

References 69 publications

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“…Fps1 (Fig. S1) is a large aquaglyceroporin consisting of a six transmembrane domain core and cytosolic N‐ and C‐ terminal domains of 255 and 139 amino acids, respectively . The N‐ and C‐terminal extensions of Fps1, which are specific for certain fungal aquaglyceroporins, are involved in regulation.…”

mentioning

confidence: 99%

The mitogen‐activated protein kinase Slt2 modulates arsenite transport through the aquaglyceroporin Fps1

Ahmadpour

Maciaszczyk-Dziubińska

Babazadeh

et al. 2016

FEBS Letters

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Arsenite is widely present in nature; therefore, cells have evolved mechanisms to prevent arsenite influx and promote efflux. In yeast (Saccharomyces cerevisiae), the aquaglyceroporin Fps1 mediates arsenite influx and efflux. The mitogen-activated protein kinase (MAPK) Hog1 has previously been shown to restrict arsenite influx through Fps1. In this study, we show that another MAPK, Slt2, is transiently phosphorylated in response to arsenite influx. Our findings indicate that the protein kinase activity of Slt2 is required for its role in arsenite tolerance. While Hog1 prevents arsenite influx via phosphorylation of T231 at the N-terminal domain of Fps1, Slt2 promotes arsenite efflux through phosphorylation of S537 at the C terminus. Our data suggest that Slt2 physically interacts with Fps1 and that this interaction depends on phosphorylation of S537. We hypothesize that Hog1 and Slt2 may affect each other's binding to Fps1, thereby controlling the opening and closing of the channel.

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confidence: 99%

The mitogen‐activated protein kinase Slt2 modulates arsenite transport through the aquaglyceroporin Fps1

Ahmadpour

Maciaszczyk-Dziubińska

Babazadeh

et al. 2016

FEBS Letters

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“…There are indications for both explications. High salinity induces glycerol as the major osmoprotectant of yeast cells (Hohmann, ), which deviates glycolytic intermediates. Consequently, less efficiently fermentable carbon sources strongly interfere with glycerol biosynthesis and salt stress adaptation and resistance (Vanacloig‐Pedros et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…There are indications for both explications. High salinity induces glycerol as the major osmoprotectant of yeast cells (Hohmann, 2015), which deviates glycolytic…”

Section: Discussionmentioning

confidence: 99%

Multilayered control of peroxisomal activity upon salt stress in Saccharomyces cerevisiae

Manzanares-Estreder

Espí-Bardisa

Alarcón

et al. 2017

Molecular Microbiology

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Peroxisomes are dynamic organelles and the sole location for fatty acid β-oxidation in yeast cells. Here, we report that peroxisomal function is crucial for the adaptation to salt stress, especially upon sugar limitation. Upon stress, multiple layers of control regulate the activity and the number of peroxisomes. Activated Hog1 MAP kinase triggers the induction of genes encoding enzymes for fatty acid activation, peroxisomal import and β-oxidation through the Adr1 transcriptional activator, which transiently associates with genes encoding fatty acid metabolic enzymes in a stress- and Hog1-dependent manner. Moreover, Na and Li stress increases the number of peroxisomes per cell in a Hog1-independent manner, which depends instead of the retrograde pathway and the dynamin related GTPases Dnm1 and Vps1. The strong activation of the Faa1 fatty acyl-CoA synthetase, which specifically localizes to lipid particles and peroxisomes, indicates that adaptation to salt stress requires the enhanced mobilization of fatty acids from internal lipid stores. Furthermore, the activation of mitochondrial respiration during stress depends on peroxisomes, mitochondrial acetyl-carnitine uptake is essential for salt resistance and the number of peroxisomes attached to the mitochondrial network increases during salt adaptation, which altogether indicates that stress-induced peroxisomal β-oxidation triggers enhanced respiration upon salt shock.

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“…The paper by Hohmann (2015) reviews recent progress in understanding osmoregulation in eukaryotes, concentrating in particular on the different roles of Hog1 in glycerol accumulation and volume recovery in yeast. Osmoregulation is critical for cell shape and turgor as well as intracellular dynamics.…”

Section: Topics Covered In This Special Issuementioning

confidence: 99%

Fungal stress biology: a preface to the Fungal Stress Responses special edition

et al. 2015

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There is currently an urgent need to increase global food security, reverse the trends of increasing cancer rates, protect environmental health, and mitigate climate change. Toward these ends, it is imperative to improve soil health and crop productivity, reduce food spoilage, reduce pesticide usage by increasing the use of biological control, optimize bioremediation of polluted sites, and generate energy from sustainable sources such as biofuels. This review focuses on fungi that can help provide solutions to such problems. We discuss key aspects of fungal stress biology in the context of the papers published in this Special Issue of Current Genetics. This area of biology has relevance to pure and applied research on fungal (and indeed other) systems, including biological control of insect pests, roles of saprotrophic fungi in agriculture and forestry, mycotoxin contamination of the food-supply chain, optimization of microbial fermentations including those used for bioethanol production, plant pathology, the limits of life on Earth, and astrobiology.

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An integrated view on a eukaryotic osmoregulation system

Cited by 118 publications

References 69 publications

The mitogen‐activated protein kinase Slt2 modulates arsenite transport through the aquaglyceroporin Fps1

The mitogen‐activated protein kinase Slt2 modulates arsenite transport through the aquaglyceroporin Fps1

Multilayered control of peroxisomal activity upon salt stress in Saccharomyces cerevisiae

Fungal stress biology: a preface to the Fungal Stress Responses special edition

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