An International, Prospective, Multicenter Evaluation of the Combination of AdvanDx Staphylococcus
            <i>Quick</i>
            FISH BC with
            <i>mecA Xpress</i>
            FISH for Detection of Methicillin-Resistant Staphylococcus aureus Isolates from Positive Blood Cultures

Salimnia, Hossein; Fairfax, Marilynn R.; Lephart, Paul; Morgan, Margie; Gilbreath, Jeremy J.; Butler-Wu, Susan M.; Templeton, Kate; Hamilton, Fiona; Wu, Fann; Buckner, Rebecca J.; Fuller, D.; Davis, Thomas E.; Abdelhamed, Ayman M.; Jacobs, Michael; Miller, Andy O.; Pfrommer, B.; Carroll, Karen C.

doi:10.1128/jcm.01811-14

Cited by 19 publications

(7 citation statements)

References 14 publications

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“…Studies have demonstrated that inappropriate empirical therapy is associated with increased hospital mortality ( 7 , 9 , 48 ). The need for rapid AST results has led to the development of several assays for ID, which cover 80 to 90% of pathogens recovered in positive blood cultures ( 12 , 24 , 49 ). However, unlike other rapid diagnostic platforms that identify organisms from positive blood culture bottles and detect genetic resistance markers, the Accelerate Pheno system is unique in its ability to identify and provide MIC and categorical phenotypic AST results in 7 h for several antimicrobial agents targeting the Gram-positive and Gram-negative organisms using the Accelerate PhenoTest BC kit.…”

Section: Discussionmentioning

confidence: 99%

Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis

et al. 2018

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We describe results from a multicenter study evaluating the Accelerate Pheno system, a first of its kind diagnostic system that rapidly identifies common bloodstream pathogens from positive blood cultures within 90 min and determines bacterial phenotypic antimicrobial susceptibility testing (AST) results within ∼7 h. A combination of fresh clinical and seeded blood cultures were tested, and results from the Accelerate Pheno system were compared to Vitek 2 results for identification (ID) and broth microdilution or disk diffusion for AST. The Accelerate Pheno system accurately identified 14 common bacterial pathogens and two Candida spp. with sensitivities ranging from 94.6 to 100%. Of fresh positive blood cultures, 89% received a monomicrobial call with a positive predictive value of 97.3%. Six common Gram-positive cocci were evaluated for ID. Five were tested against eight antibiotics, two resistance phenotypes (methicillin-resistant Staphylococcus aureus and Staphylococcus spp. [MRSA/MRS]), and inducible clindamycin resistance (MLSb). From the 4,142 AST results, the overall essential agreement (EA) and categorical agreement (CA) were 97.6% and 97.9%, respectively. Overall very major error (VME), major error (ME), and minor error (mE) rates were 1.0%, 0.7%, and 1.3%, respectively. Eight species of Gram-negative rods were evaluated against 15 antibiotics. From the 6,331 AST results, overall EA and CA were 95.4% and 94.3%, respectively. Overall VME, ME, and mE rates were 0.5%, 0.9%, and 4.8%, respectively. The Accelerate Pheno system has the unique ability to identify and provide phenotypic MIC and categorical AST results in a few hours directly from positive blood culture bottles and support accurate antimicrobial adjustment.

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Section: Discussionmentioning

confidence: 99%

Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis

et al. 2018

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“…This approach may be useful in the case of specific microbial pathogens such as Staphylococcus aureus and in cases where a faster time to positivity is desirable . This approach may also help to target appropriate antimicrobial therapy . Moreover, these authenticated protocols may allow for increased use of FISH probes in clinical labs that lack specific expertise.…”

Section: Diagnostic Methodsmentioning

confidence: 99%

Microbiological diagnosis of device‐related biofilm infections

Larsen

Lorenzen

et al. 2017

APMIS

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Medical device-related infections cause undue patient distress, increased morbidity and mortality and pose a huge financial burden on healthcare services. The pathogens are frequently distributed heterogeneously in biofilms, which can persist without being effectively cleared by host immune defenses and antibiotic therapy. At present, there is no 'gold standard' available to reveal the presence of device-related biofilm infections. However, adequate sample collection and logistics, standardised diagnostic methods, and interpretation of results by experienced personnel are important steps in efficient diagnosis and treatment of these infections. The focus of this mini review is on prosthethic joint and cardiovascular implantable device infections, which exemplify permanent devices that are placed in a sterile body site. These device-related infections represent some of the most challenging in terms of both diagnosis and treatment.

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“…Molecular methods can also contribute to a rapid and specific identification of microorganisms. However, concerning susceptibility testing, scarce useful information can be provided since only a few genes codifying for resistance-associated proteins are yet known (Salimnia et al, 2014 ; Ceyssens et al, 2016 ). A global susceptibility profile is impossible to be obtained by molecular biology unless whole genome sequencing tools are used (Zankari et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Potential Impact of Flow Cytometry Antimicrobial Susceptibility Testing on the Clinical Management of Gram-Negative Bacteremia Using the FASTinov® Kit

et al. 2017

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Laboratory assessment of antimicrobial susceptibility is a prerequisite for adequate management of infections. The aim of this research was to evaluate the performance of the novel FASTinov® kit for antimicrobial susceptibility testing (AST) of Gram negative bacilli directly on positive blood cultures. One hundred and two positive blood cultures from patients of a Portuguese University Hospital were included. AST were performed with routine method, Vitek2, with FASTinov® kit, and with the gold standard microdilution. Bacteria directly extracted from blood cultures were used to inoculate the FASTinov® kit. Time-to-result as well as the number of patients receiving initially inappropriate therapy (and those in whom de-escalation would have been done) and length of stay (LOS) was recorded. Seventy percent of patients were over 70 years old and 18.6% were admitted in intensive care units. Regarding the isolates, 88.2% were Enterobacteriaceae, 9.8% Pseudomonas spp. and 1% Acinetobacter spp. Extended spectrum β-lactamases producing-Enterobacteriaceae were found in 7.8% of cases and 10.8% were multi-drug resistant. Fifty-one hours was the mean of time-to-result for routine test (Vitek2) vs. 2 h response regarding Fastinov® test. The overall agreement between FASTinov® and the reference microdilution method was 98%. According to the susceptibility phenotype, 16.7% of patients received initially inappropriate therapy and the mean hospital LOS of these patients was significantly higher. FASTinov® kit revealed an excellent correlation with the AST standard method and provided much earlier results than Vitek2.

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An International, Prospective, Multicenter Evaluation of the Combination of AdvanDx Staphylococcus Quick FISH BC with mecA Xpress FISH for Detection of Methicillin-Resistant Staphylococcus aureus Isolates from Positive Blood Cultures

Cited by 19 publications

References 14 publications

Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis

Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis

Microbiological diagnosis of device‐related biofilm infections

Potential Impact of Flow Cytometry Antimicrobial Susceptibility Testing on the Clinical Management of Gram-Negative Bacteremia Using the FASTinov® Kit

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