An introduction to MAS NMR spectroscopy on oriented membrane proteins

Glaubitz, Clemens

doi:10.1002/(sici)1099-0534(2000)12:3<137::aid-cmr3>3.0.co;2-f

Cited by 14 publications

(6 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Simulation of NMR Spectra. MAS spectra at 3 kHz of the AAO constructions were simulated in the time domain using an equivalent approach to those described by Glaubitz and Sizun and Bechinger. , The cylindrical distribution of lipids is simulated by a series of 360 crystallites describing a circle in the plane perpendicular to the rotor axis corresponding to the distribution of the membrane normal. To fit the experimental data, an additional Gaussian distribution of the crystallites' orientation is introduced to take into account for AAO surface irregularities or a mismatch between the lipid bilayer alignment inside the pore cylinder.…”

Section: Methodsmentioning

confidence: 99%

“…Structural information can be obtained when using aligned biomimetics membranes specially designed for NMR studies, , like glass plates or bicelles allowing for high-resolution spectra. Recently, aligned bilayers inserted into the nanotubes arrays of anodic aluminum oxide (AAO) have been investigated by solid-state NMR. , An additional refinement consists of combining the oriented samples with the magic angle spinning technique (MAS) for better sensitivity and finest structural information. , …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tethered or Adsorbed Supported Lipid Bilayers in Nanotubes Characterized by Deuterium Magic Angle Spinning NMR Spectroscopy

2005

View full text Add to dashboard Cite

2H solid-state NMR experiments were performed under magic angle spinning on lipid bilayers oriented into nanotubes arrays, as a new method to assess the geometrical arrangement of the lipids. Orientational information is obtained from the intensities of the spinning sidebands. The lipid bilayers are formed by fusion of small unilamellar vesicles of DMPC-d54 inside a nanoporous anodic aluminum oxide, either by direct adsorption on the support or by tethering through a streptavidin/biotin linker. The results support that the quality of the lipid bilayers alignment is clearly in favor of the tethering rather than an adsorbed strategy.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tethered or Adsorbed Supported Lipid Bilayers in Nanotubes Characterized by Deuterium Magic Angle Spinning NMR Spectroscopy

2005

View full text Add to dashboard Cite

show abstract

“…Similarly, the DOP of two rank‐2 tensors is

T r s = true(\sum i, j = x, y, z true(e i \otimes e j true) T i j true) .. t r s,

then

T 00 true{2 true} = - \frac{1}{\sqrt{3}} true(T x x + T y y + T z z true),

T 10 true{2 true} = - \frac{1}{\sqrt{2}} i true(T x y - T y x true),

T 1 \pm 1 true{2 true} = - \frac{1}{2} true{T z x - T x z \pm i true(T z y - T y z true) true},

T 20 true{2 true} = \frac{1}{\sqrt{6}} true{3 T z z - true(T x x + T y y + T z z true) true},

T 2 \pm 1 true{2 true} = \mp \frac{1}{2} true{T x z <...>

…”

Section: Spherical Tensormentioning

confidence: 99%

“…The quadrupole interaction of a nuclear spin I > 1/2 ,

H Q = \frac{e Q}{2 I (2 I - 1) ℏ} I \cdot Γ \cdot I,

describes the coupling of the nuclear electric quadrupole moment eQ with the electric field gradient at the site of the nucleus. Tensor

boldΓ

is symmetric and traceless.…”

Section: Tensors In Nmr Hamiltonianmentioning

confidence: 99%

Cartesian and Spherical Tensors in NMR Hamiltonians

Man

2013

Concepts Magnetic Resonance

View full text Add to dashboard Cite

NMR Hamiltonians are anisotropic due to their orientation dependence with respect to the strong, static magnetic field. They are best represented by product of two rank‐2 tensors: one is the space‐part tensor T and the other is the spin‐part tensor A. We reformulated the dot product of Cartesian tensors and the dyadic product of spherical tensors in NMR Hamiltonian as the double contraction of these two tensors. As the double contract has two definitions (double inner product and double outer product of two rank‐2 tensors), there are two sets of spherical tensor components in terms of Cartesian tensor components for any rank‐2 tensor, two Cartesian Hamiltonians, and two spherical Hamiltonians. We succeeded in determining the spherical components of tensors A and T that verify the Cartesian Hamiltonian defined by the double inner product of two rank‐2 tensors and the spherical Hamiltonian defined by the double outer product of two rank‐2 tensors. In particular, we established the spherical components of space‐part tensor T in terms of Cartesian tensor components provided by Cook and De Lucia. Throughout the article, Wigner active rotation matrix is used to illustrate the active rotation of spherical vector, spherical harmonics, and spherical tensor as well as the passive rotation via their rotational invariants. © 2014 Wiley Periodicals, Inc. Concepts Magn Reson Part A 42A: 197–244, 2013.

show abstract

“…Two main directions have emerged: (i) randomly oriented samples in combination with magic angle spinning (MAS, [18]) as reviewed in [19,20], and (ii) oriented samples under static [21,22] or spinning conditions [23,24]. The scalability of the first approach to study larger proteins is straightforward.…”

mentioning

confidence: 99%

Solid-state NMR of (membrane) protein complexes: Novel methods and applications

Andronesi¹

View full text Add to dashboard Cite

and his advice during my studies. Also, all members of the PhD comity that red and evaluated this thesis.Prof. dr. Christian Griesinger for accepting me in his NMR department at Max Planck Institute for Biophysical Chemistry, Göttingen, all scientific support and interest in my research. It was a great advantage to work in such an environment, having access to the most modern equipment and coming in contact with so many challenging projects. Equally for the nice atmosphere, including the yearly departmental excursions.Dr. Marc Baldus for all guidance during my PhD studies from the level of introducing me to the field of solid-state NMR up to exploring the limits of what the modern instrumentation can do. My first contact with NMR was in the lab of Prof. de Groot from Leiden University where I spent few months during my master studies and at the end of my stay I met Marc who gave me a short introduction in GAMMA and impressed me with the style the he just brought fresh from MIT. At that time, I have seen the very promising applications of solid-state NMR to biology. I was happy for the opportunity to become a PhD student in the group that Marc started in Göttingen and be involved in development of new methods and applications for the study of membrane proteins. These years have been a fruitful and stimulating experience.Prof. dr. Huub J. M. de Groot from Gorlaeus Laboratories, Leiden University, The Netherlands, for giving me the first chance to work with NMR. I spent many nice moments in Leiden in the company of his family.Dr. Stefan Becker for many discussions about molecular biology and importance of function-structure relationship. In particular, for the very interesting Phospolamban project that he proposed and all efforts that he pursued during its study.The group of Prof. dr. Eckhard Mandelkow from Max-Planck-Unit for Structural Molecular Biology in Hamburg for the protein Tau paired helical filaments. In particular, Dr. Martin von Bergen for his constant support and interest that considerably motivated and inspired this project.Brigitta Angerstein that always helped me in dealing with the most challenging samples -membrane proteins reconstituted in oriented lipid bilayers. Her expertise and 'magic' hands finally made the samples to orient, roll and spin. Not last, for her friendly and carrying feelings.Als Schüler mit einer Leidenschaft für Physik las ich viele Bücher über die Geschichte der Physik. Darin tauchte der Name eines Ortes besonders oft auf: Göttingen. Vielleicht war es wegen der großen Forscher und ihrer Leistungen, die in diesen Büchern beschrieben wurden, daß ich Physiker werden wollte. Zu dieser Zeit hätte ich aber nie gedacht, daß ich an einem Ort promovieren würde, dessen Name so 'resoniert' wie Göttingen.Ich hoffe, ich kann etwas von dem Geist mit mir nehmen, der Größen wie Gauss, Planck oder Heisenberg genährt hat.Este o experient ¸ȃ deosebitȃ contactul cu oamenii altor t ¸ȃri şi culturi. Pe lȃngȃ realizȃrile din timpul doctoratului am avut ocazia sȃ mȃ confrunt cu multe situat ¸ii. Mai ...

show abstract

An introduction to MAS NMR spectroscopy on oriented membrane proteins

Cited by 14 publications

References 47 publications

Tethered or Adsorbed Supported Lipid Bilayers in Nanotubes Characterized by Deuterium Magic Angle Spinning NMR Spectroscopy

Tethered or Adsorbed Supported Lipid Bilayers in Nanotubes Characterized by Deuterium Magic Angle Spinning NMR Spectroscopy

Cartesian and Spherical Tensors in NMR Hamiltonians

Solid-state NMR of (membrane) protein complexes: Novel methods and applications

Contact Info

Product

Resources

About