An Introduction to Mycobacterial Taxonomy, Structure, Drug Resistance, and Pathogenesis

Rastogi, Nalin

doi:10.1007/978-88-470-2091-7_8

Cited by 5 publications

(3 citation statements)

References 154 publications

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“…Second, validated protocols for electrophoresis and internal standards have not been defined [ 17 , 29 ]. Lastly, published tables present patterns which differ within a range of 5–15 bp and lack patterns for recently described species [ 11 , 14 , 16 ]. The aim of this study was to determine whether PRA- hsp65 of mycobacterial isolates provides sufficiently reliable species identification to enable it to be used as the routine methodology in a reference laboratory.…”

Section: Introductionmentioning

confidence: 88%

Reliable identification of mycobacterial species by PCR-restriction enzyme analysis (PRA)-hsp65 in a reference laboratory and elaboration of a sequence-based extended algorithm of PRA-hsp65patterns

et al. 2008

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Background: Identification of nontuberculous mycobacteria (NTM) based on phenotypic tests is time-consuming, labor-intensive, expensive and often provides erroneous or inconclusive results. In the molecular method referred to as PRA-hsp65, a fragment of the hsp65 gene is amplified by PCR and then analyzed by restriction digest; this rapid approach offers the promise of accurate, cost-effective species identification. The aim of this study was to determine whether species identification of NTM using PRA-hsp65 is sufficiently reliable to serve as the routine methodology in a reference laboratory.

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Section: Introductionmentioning

confidence: 88%

Reliable identification of mycobacterial species by PCR-restriction enzyme analysis (PRA)-hsp65 in a reference laboratory and elaboration of a sequence-based extended algorithm of PRA-hsp65patterns

et al. 2008

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“…About 2 million humans die from tuberculosis (TB) each year, and it is estimated that about one-third of the world's population is infected with Mycobacterium tuberculosis (20). The disease is most common in developing countries and is spreading fast because of the human immunodeficiency virus pandemic.…”

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confidence: 99%

Molecular Epidemiology of Mycobacterium tuberculosis in Western Sweden

et al. 2004

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The genetic diversity of Mycobacterium tuberculosis isolates among patients from Sweden was determined by a combination of two PCR-based techniques (spoligotyping and variable number of tandem repeats analysis). It resulted in a clustering of 23.6% of the isolates and a rate of recent transmission of 14.1%. The clustered isolates mainly belonged to the Haarlem family (23.2%), followed by the Beijing (9.8%), Latin American and Mediterranean (LAM; 8%), and East African-Indian (EAI; 6.2%) families. A comparison of the spoligotypes with those in the international spoligotyping database showed that 62.5% of the clustered isolates and 36.6% of all isolates typed were grouped into six major shared types. A comparison of the spoligotypes with those in databases for Scandinavian countries showed that 33% of the isolates belonged to an ill-defined T family, followed by the EAI (22%), Haarlem (20%), LAM (11%), Central Asian (5%), X (5%), and Beijing (4%) families. Both the highest number of cases and the proportion of clustered cases were observed in patients ages 15 to 39 years. Nearly 10% of the isolates were resistant to one or more drugs (essentially limited to isoniazid monoresistance). However, none of the strains were multidrug resistant. Data on the geographic origins of the patients showed that more than two-thirds of the clustered patients with tuberculosis were foreign-born individuals or refugees. These results are explained on the basis of both the historical links within specific countries and recently imported cases of tuberculosis into Sweden.

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“…Images were captured in Polaroid films and restriction patterns were analyzed using the GelCompar II program v. 2.5 (AppliedMaths, Sint-Marten Latten, Belgium). Calculated restriction fragment sizes were compared to published patterns (13,15) and to the patterns reported on the PRAsite (http://app.chuv.ch/prasite).…”

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confidence: 99%

Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance

Moraes

Chimara

Telles

et al. 2008

Braz. J. Microbiol.

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Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates.

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An Introduction to Mycobacterial Taxonomy, Structure, Drug Resistance, and Pathogenesis

Cited by 5 publications

References 154 publications

Reliable identification of mycobacterial species by PCR-restriction enzyme analysis (PRA)-hsp65 in a reference laboratory and elaboration of a sequence-based extended algorithm of PRA-hsp65patterns

Reliable identification of mycobacterial species by PCR-restriction enzyme analysis (PRA)-hsp65 in a reference laboratory and elaboration of a sequence-based extended algorithm of PRA-hsp65patterns

Molecular Epidemiology of Mycobacterium tuberculosis in Western Sweden

Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance

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