An Introduction to the Process of Cell, Tissue, and Organ Differentiation, and Production of Secondary Metabolites

Ramawat, K. G.

doi:10.1007/978-3-030-30185-9_35

Cited by 7 publications

(2 citation statements)

References 82 publications

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“…For instance, up to a 7.43-fold increase in rosmarinic acid was observed in M. officinalis when treated with methyl jasmonate [ 6 ]. Moreover, studies have also employed numerous in vitro approaches such as callus culture, cell suspension culture, and bioreactors to enhance secondary metabolite production in medicinal and aromatic plants [ 7 , 8 , 9 , 10 ]. These attempts will further increase to fulfill the rising global demand for these secondary metabolites and essential oils across industries.…”

Section: Introductionmentioning

confidence: 99%

Systematic Identification of Suitable Reference Genes for Quantitative Real-Time PCR Analysis in Melissa officinalis L

Bharati

Sen

Kumar

et al. 2023

Plants

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Melissa officinalis L. is well known for its lemon-scented aroma and various pharmacological properties. Despite these valuable properties, the genes involved in the biosynthetic pathways in M. officinalis are not yet well-explored when compared to other members of the mint family. For that, gene expression studies using quantitative real-time PCR (qRT-PCR) are an excellent tool. Although qRT-PCR can provide accurate results, its accuracy is highly reliant on the expression and stability of the reference gene used for normalization. Hence, selecting a suitable experiment-specific reference gene is very crucial to obtain accurate results. However, to date, there are no reports for experiment-specific reference genes in M. officinalis. Therefore, in the current study, ten commonly used reference genes were assessed for their suitability as optimal reference genes in M. officinalis under various abiotic stress conditions and different plant organs. The candidate genes were ranked based on BestKeeper, comparative ΔCt, geNorm, NormFinder, and RefFinder. Based on the results, we recommend the combination of EF-1α and GAPDH as the best reference genes to normalize gene expression studies in M. officinalis. On the contrary, HLH71 was identified as the least-performing gene. Thereafter, the reliability of the optimal gene combination was assessed by evaluating the relative gene expression of the phenylalanine ammonia lyase (PAL) gene under two elicitor treatments (gibberellic acid and jasmonic acid). PAL is a crucial gene involved directly or indirectly in the production of various economically important secondary metabolites in plants. Suitable reference genes for each experimental condition are also discussed. The findings of the current study form a basis for current and future gene expression studies in M. officinalis and other related species.

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Section: Introductionmentioning

confidence: 99%

Systematic Identification of Suitable Reference Genes for Quantitative Real-Time PCR Analysis in Melissa officinalis L

Bharati

Sen

Kumar

et al. 2023

Plants

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“…Many efforts have been made to produce important medicinal compounds through the culture of plant roots and stem due to the production of more secondary metabolites in differentiated tissues. As one of the widely used methods to increase the production of various compounds in plants, the induction of hairy roots has received much attention today (Ramawat 2021). The hairy root is a type of plant disease that is caused by the transformation of plant cells by Rhizobium rhizogenes, which is a Gram-negative soil bacterium.…”

Section: Introductionmentioning

confidence: 99%

Effect of Acetosyringone, Sucrose and Nutrients on Transgenic Hairy Root Induction in Chenopodium quinoa using different Rhizobium rhizogenesstrains

Massah

Rabiei

2023

Preprint

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Quinoa is a plant with special nutritional and biological properties and tolerant in strict environmental conditions, which can be considered to a strategic product for the future food supply of mankind, as a pseudocereal. Quinoa root is rich in bioactive compounds with antimicrobial, antioxidant activity and diverse medicinal and nutritional properties that can have a potential for commercialization. The use of cultivation and propagation techniques in controlled laboratory conditions, such as the induction of differentiated tissues of hairy roots, allows the production of plants and the rapid and massive access to secondary metabolites. In this research, the induction of hairy roots was evaluated using four Rhizobium rhizogenes strains, including A4, A7, 11325, and ATCC 15834, in quinoa and the effect of different concentrations of acetosyringone and sucrose and removal of macro elements in the culture medium in increasing the rooting performance of R. rhizogenes was investigated. The results showed the different effects of the mutual effect of different concentrations of sucrose and acetosyringone, as well as the commonly used elements of the half strength MS culture medium on the biomass of hairy roots. The most optimal conditions for the induction of hairy roots culture were obtained in half strength MS culture medium, containing 30 g/l sucrose and 1 mM acetosyringone and without NH4NO3 salt. This efficient protocol provides a basis for future studies to be able to use the induced hairy root as a source of valuable secondary metabolites of quinoa root and create a new approach for exploiting the nutritional, medicinal and health compounds of the quinoa plant.

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Magnetic Biohybrid Robots as Efficient Drug Carrier to Generate Plant Cell Clones

Mayorga‐Martinez

Pumera

2022

Small

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Micro/nanorobots represent a new generation of micromachines that can accomplish various tasks, such as loading and transporting specific targets or pharmaceuticals for a given application. Biohybrid robots consisting of biological cells (bacteria, sperm, and microalgae) combined with inorganic particles to control or propel their movement are of particular interest. The skeleton of these biohybrid robots can be used to load biomolecules. In this work, the authors create biohybrid robots based on tomato plants by coculturing ferromagnetic nanoparticles (Fe3O4) with tomato callus cells. The tomato‐based biohybrid robots (Tomato‐Biobots) containing Fe3O4 nanoparticles are driven by a transversely rotating magnetic field. In addition, biohybrid robots are used to load vitamin C, to generate clones of tomato cells. It is shown that the presence of Fe3O4 does not affect the growth of tomato callus. This study opens a wide range of possibilities for the use of biohybrid robots@Fe3O4 to deliver conventional agrochemicals, including fertilizers, pesticides, and herbicides, and allows for a gradual and sustained release of nutrients and agrochemicals, leading to precise dosing that reduces the amount of agrochemicals used. This conceptually new type of micromachine with application to plants and agronomy shall find broad use in this field.

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An Introduction to the Process of Cell, Tissue, and Organ Differentiation, and Production of Secondary Metabolites

Cited by 7 publications

References 82 publications

Systematic Identification of Suitable Reference Genes for Quantitative Real-Time PCR Analysis in Melissa officinalis L

Systematic Identification of Suitable Reference Genes for Quantitative Real-Time PCR Analysis in Melissa officinalis L

Effect of Acetosyringone, Sucrose and Nutrients on Transgenic Hairy Root Induction in Chenopodium quinoa using different Rhizobium rhizogenesstrains

Magnetic Biohybrid Robots as Efficient Drug Carrier to Generate Plant Cell Clones

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