An investigation into the effect of culture conditions on fucoxanthin production using the marine microalgae Phaeodactylum tricornutum

McClure, Dale D.; Luiz, Audrey; Gerber, Blandine; Barton, G.W.; Kavanagh, John M.

doi:10.1016/j.algal.2017.11.015

Cited by 158 publications

(133 citation statements)

References 25 publications

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“…This implies that the availability of phosphorous in 30% v/v POME does not limit the growth of P. tricornutum under our experimental conditions. This finding was supported by a previous study who reported that a high amount of external nitrate increased the fucoxanthin productivity, growth rate, and biomass production of P. tricornutum and Odontella aurita cultivated on a modified f/2 and a modified L1 medium, respectively (Xia et al 2013;McClure et al 2018). Furthermore, early nitrogen limitation reduced the fucoxanthin productivity of P. tricornutum cultivated on a modified f/2 medium (Alipanah et al 2015).…”

Section: Discussionsupporting

confidence: 79%

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Environmental and nutrient conditions influence fucoxanthin productivity of the marine diatom Phaeodactylum tricornutum grown on palm oil mill effluent

et al. 2018

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Palm oil mill effluent (POME) is a type of wastewater posing large problems when discharged in the environment. Yet, due to its high nutrient content, POME may offer opportunities for algal growth and subsequent harvesting of high-value products. The marine diatom Phaeodactylum tricornutum is a potential feedstock diatom for bioactive compounds such as the carotenoid fucoxanthin, which has been shown to have pharmaceutical applications. The aim of this paper was to evaluate the growth and fucoxanthin productivity of P. tricornutum grown on POME, as a function of light intensity, temperature, salinity, and nutrient enrichment. High-saturating irradiance (300 μmol photons m −2 s −1) levels at 25°C showed highest growth rates but decreased the fucoxanthin productivity of P. tricornutum. Box-Behnken response surface methodology revealed that the optimum fucoxanthin productivity was influenced by temperature, salinity, and the addition of urea. Nutrient enrichment by phosphorus did not enhance cell density and fucoxanthin productivity, while urea addition was found to stimulate both. We conclude that POME wastewater, supplemented with urea, can be considered as the potential medium for P. tricornutum to replace commercial nutrients while producing high amounts of fucoxanthin.

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Section: Discussionsupporting

confidence: 79%

“…In addition, the biomass production, growth, and fucoxanthin productivity of P. tricornutum depend on nutrient concentration and the balance between relevant nitrogen and phosphorus (N/P) species in the media (Choi and Lee 2015;Whitton et al 2016;McClure et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Environmental and nutrient conditions influence fucoxanthin productivity of the marine diatom Phaeodactylum tricornutum grown on palm oil mill effluent

et al. 2018

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“…For species belonging to Bacillariophyta, carotenoid content is mainly constituted of fucoxanthin, a photosynthetic pigment. As expected there is higher fucoxanthin in LL condition in most species which is in agreement with the litterature [35,54,55]. In Chlorophyte species, lutein is the major carotenoid.…”

Section: Carotenoidssupporting

confidence: 88%

“…In an aquatic environment and especially in tropical areas, microalgae are submitted to strong light variation and have to quickly adapt to light excess or limitation. The effect of light on antioxidants production, especially carotenoids, is known to be complex and species specific [31][32][33][34][35][36]. While many studies focus on the effect of light on specific antioxidant molecules, investigations about its effect on global antioxidant activity of microalgae are scarce.…”

Section: Introductionmentioning

confidence: 99%

Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae

Coulombier¹,

Nicolau

Déan

et al. 2020

Marine Drugs

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Twelve microalgae species isolated in tropical lagoons of New Caledonia were screened as a new source of antioxidants. Microalgae were cultivated at two light intensities to investigate their influence on antioxidant capacity. To assess antioxidant property of microalgae extracts, four assays with different modes of action were used: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-éthylbenzothiazoline-6-sulphonique) (ABTS), oxygen radical absorbance capacity (ORAC), and thiobabituric acid reactive substances (TBARS). This screening was coupled to pigment analysis to link antioxidant activity and carotenoid content. The results showed that none of the microalgae studied can scavenge DPPH and ABTS radicals, but Chaetoceros sp., Nephroselmis sp., and Nitzschia A sp. have the capacity to scavenge peroxyl radical (ORAC) and Tetraselmis sp., Nitzschia A sp., and Nephroselmis sp. can inhibit lipid peroxidation (TBARS). Carotenoid composition is typical of the studied microalgae and highlight the siphonaxanthin, detected in Nephroselmis sp., as a pigment of interest. It was found that xanthophylls were the major contributors to the peroxyl radical scavenging capacity measured with ORAC assay, but there was no link between carotenoids and inhibition of lipid peroxidation measured with TBARS assay. In addition, the results showed that light intensity has a strong influence on antioxidant capacity of microalgae: Overall, antioxidant activities measured with ORAC assay are better in high light intensity whereas antioxidant activities measured with TBARS assay are better in low light intensity. It suggests that different antioxidant compounds production is related to light intensity.

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“…Analyzing all the results obtained, NL batch was confirmed as being richer in carotenoids (fucoxanthin) compared to the batch produced under LED supplementation. Other authors have also demonstrated that lower light intensities were more favorable to produce P. tricornutum biomass with higher carotenoid content [26]. Therefore, NL T. lutea biomass was selected to proceed with the exhaustive optimization of the extraction conditions to maximize the recovery of carotenoids (fucoxanthin) using PLE.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous extraction and purification of fucoxanthin from Tisochrysis lutea microalgae using compressed fluids

Gallego

Tardif

Parreira³

et al. 2020

J of Separation Science

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The marine microalga Tisochrysis lutea, a Haptophyta with a thin cell wall and currently used mainly in aquaculture is a potential source of several bioactive compounds of interest such as carotenoids. In the present study, the simultaneous extraction and purification of fucoxanthin, the main carotenoid from T. lutea, was optimized using pressurized fluid extraction followed by in-cell purification. An experimental design was employed to maximize carotenoids' extraction; the experimental factors chosen were: (i) percentage of ethanol/ethyl acetate (0-100 %), (ii) temperature (40-150 • C), and (iii) number of static extraction cycles (1-3). The maximum carotenoids' recovery, mainly fucoxanthin, was obtained with pure ethyl acetate at 40 • C using one extraction cycle, achieving values of 132.8 mg of carotenoids per gram of extract. Once the optimum extraction conditions were confirmed, in-cell purification strategies using different adsorbents were developed to obtain fucoxanthin-enriched extracts. Activated charcoal showed potential retention of chlorophylls allowing an effective purification of fucoxanthin in the obtained extracts. Chemical characterization of extracts was carried out by reversed-phase high-performance liquid chromatography with diode array detection. Therefore, a selective fractionation of high value compounds was achieved using the proposed green downstream platform based on the use of compressed fluids. K E Y W O R D Sactivated charcoal, adsorbents, fucoxanthin, purification, Tisochrysis lutea

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An investigation into the effect of culture conditions on fucoxanthin production using the marine microalgae Phaeodactylum tricornutum

Cited by 158 publications

References 25 publications

Environmental and nutrient conditions influence fucoxanthin productivity of the marine diatom Phaeodactylum tricornutum grown on palm oil mill effluent

Environmental and nutrient conditions influence fucoxanthin productivity of the marine diatom Phaeodactylum tricornutum grown on palm oil mill effluent

Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae

Simultaneous extraction and purification of fucoxanthin from Tisochrysis lutea microalgae using compressed fluids

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