An Investigation on the Regenerative Effects of Intra Articular Injection of Co-Cultured Adipose Derived Stem Cells with Chondron for Treatment of Induced Osteoarthritis

Jacer, S; Shafaei, Hajar; J, Soleimani Rad

doi:10.15171/apb.2018.035

Cited by 11 publications

(8 citation statements)

References 65 publications

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“…So, the changes of GAG levels in synovial fluids reflect catabolic activity in cartilage ECM [42]. In this study, overexpression of IGF-1 and miR-140 each individually reduces the content of GAG in synovial fluid, decreases COL1A2 (fibrocartilaginous markers) and COL10A1 (cartilage hypertrophy markers) synthesis [43, 44], and increases ACAN and COL2A1 synthesis compared with the negative control group (pBudCE4.1 transfection group) which were beneficial for cartilage repair, and fewer GAG in the synovial fluid, less COL1A2 and COL10A1, and more ACAN and COL2A1 synthesis were detected in IGF-1 combined with miR-140 group. These results imply that the repair tissue filling in the cartilage defects possesses characteristics of hyaline cartilage, and IGF-1 and miR-140 have synergistic effects for better therapeutic efficacy.…”

Section: Discussionmentioning

confidence: 99%

^pNNS-Conjugated Chitosan Mediated IGF-1 and miR-140 Overexpression in Articular Chondrocytes Improves Cartilage Repair

Zhao

Zhang

Jia

et al. 2019

BioMed Research International

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The aim of the present study was to investigate the effects of phosphorylatable nucleus localization signal linked nucleic kinase substrate short peptide (NNSp)-conjugated chitosan (NNSp-CS) mediated miR-140 and IGF-1 in both rabbit chondrocytes and cartilage defects model. NNSp-CS was combined with pBudCE4.1-IGF-1, pBudCE4.1-miR-140, and negative control pBudCE4.1 to form pDNA/NNSp-CS complexes. Then these complexes were transfected into chondrocytes or injected intra-articularly into the knee joints. High levels of IGF-1 and miR-140 expression were detected both in vitro and in vivo. Compared with pBudCE4.1 group, in vitro, the transgenic groups significantly promoted chondrocyte proliferation, increased glycosaminoglycan (GAG) synthesis, and ACAN, COL2A1, and TIMP-1 levels, and reduced the levels of nitric oxide (NO), MMP-13, and ADAMTS-5. In vivo, the exogenous genes enhanced COL2A1, ACAN, and TIMP-1 expression in cartilage and reduced cartilage Mankin score and the contents of NO, IL-1β, TNF-α, and GAG contents in synovial fluid of rabbits, MMP-13, ADAMTS-5, COL1A2, and COL10A1 levels in cartilage. Double gene combination showed better results than single gene. This study indicate that NNSp-CS is a better gene delivery vehicle in gene therapy for cartilage defects and that miR-140 combination IGF-1 transfection has better biologic effects on cartilage defects.

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Section: Discussionmentioning

confidence: 99%

^pNNS-Conjugated Chitosan Mediated IGF-1 and miR-140 Overexpression in Articular Chondrocytes Improves Cartilage Repair

Zhao

Zhang

Jia

et al. 2019

BioMed Research International

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“…Furthermore Jacer et al . [ 26 ] reported articular surfaces fibrillations, decreased cartilage thickness and loss of tidemarks integrity in the knee joints of osteoarthritic rats, 6 weeks after OA induction by intra-articular injection of collagenase II.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Intra-articular versus Intravenous Injection of Mesenchymal Stem Cells on Experimentally-Induced Knee Joint Osteoarthritis

Mostafa

Korayem

Fekry

et al. 2021

Journal of Microscopy and Ultrastructure

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Background: Osteoarthritis (OA) is a chronic degenerative debilitating disease, primarily affects joints, particularly weight-bearing areas. The surface layer of the articular cartilage breaks down and wears away leading to rubbing of bones, pain, swelling, and joint stiffness. Aim and Objectives: This study investigates the possible therapeutic effects of intra-articular versus intravenous injection of umbilical cord blood mesenchymal stem cells (UCB-MSCs) against mono-iodoacetate-induced OA of the knee joints in male albino rats, using histological and immunohistochemical techniques. Materials and Methods: Thirty male adult albino rats were randomized into five groups as follows: Group (I) and (II): Served as control. Group (III): Osteoarthritic group. Group IV: Osteoarthritic and intraarticularly-injected MSCs. Group V: Osteoarthritic and intravenously-injected MSCs. Animals were sacrificed 1 month after stem cell injection, the right knee was prepared for histological techniques (Hematoxylin and Eosin and Toluidine blue stains) and immunohistochemical technique (Bax stain). Prussian blue stain was used to assess homing of MSCs in Groups IV and V. Results: Knee joint surface was irregular, fissured, and fragmented in Group III. In Groups IV and V, affected area was filled with newly formed tissue. Toluidine blue showed a decrease in matrix staining in Group III compared to both control and MSCs-treated groups. Chondrocytes in Group III showed strong Bax immunoreactivity and this reaction decreased in Group IV and V; however, Group V immunoreactivity was more than Group IV. Prussian blue stain showed labeled UCB-MSCs in many chondrocytes in Group IV and few chondrocytes in Group V. Conclusion: Intraarticularly-injected UCB-MSCs showed better healing of knee OA than intravenously-injected UCB-MSCs.

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“…This suggests that chondrocyte/alginate spheres even with genetically modified chondrocytes can be implanted into the defective regions of osteochondral articular cartilage to promote the repair of defective cartilage in vivo [30]. Recent studies have shown that co-administration of chondrons and adipose-derived stem cells or MSCs [52,53] increases articular hyaline cartilage formation. In this study, we found that implantation with chondrons/chondrocytes alginate spheres significantly accelerated the repairs of defective knee cartilage in rabbits by increasing the cartilage matrix and thicknesses and reducing the Wakitani scores in a trend of time dependence.…”

Section: Discussionmentioning

confidence: 99%

Combination of chondrocytes with chondrons improves Proteoglycan and Collagen-2 production to promote the repairs of defective knee cartilage in rabbits

Duan

Zhao

Ren

et al. 2020

Preprint

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Background: Chondrons are composed of chondrocytes and the surrounding pericellular matrix (PCM) and function to enhance chondrocyte-mediated cartilage tissue engineering. This study aimed at investigating the potential effect of combined chondrocytes with chondrons on the production of proteoglycan and collagen-II (Col-2) and the repair of defective knee cartilage in rabbits.Methods: Chondrocytes and chondrons were isolated from the knee cartilage of rabbits, and cultured alone or co-cultured for varying periods in vitro. Their morphology was characterized by histology and immunohistochemistry. The levels of aggrecan (AGG), Col-2 and glycosaminoglycan (GAG) expression were quantified by qRT-PCR, Alcian Blue-based precipitation and ELISA. The effect of combined chondrocytes with chondrons in alginate spheres on the repair of defective knee cartilage was examined in rabbits.Results: The isolated chondrocytes and chondrons displayed unique morphology and began to proliferate on day 3 and 6 post culture, respectively, accompanied by completely degenerated PCM on day 6 post culture. Evidently, chondrocytes had stronger proliferation capacity than chondrons. Longitudinal analyses indicated that culture of chondrons, but not chondrocytes, increased AGG mRNA transcripts and GAG levels with time and Col-2 mRNA transcripts only on day 3 post culture. Compared with chondrocytes or chondrons alone, co-culture of chondrocytes and chondrons significantly up-regulated AGG and Col-2 expression and GAG production, particularly at a ratio of 1:1. Implantation with chondrocytes and chondrons at 1:1 significantly promoted the repair of defective knee cartilage in rabbits, accompanied by reduced the Wakiteni scores with time. Conclusion: Combined chondrons with chondrocytes promoted the production of proteoglycan and Col-2 and the repair of defective knee cartilage in rabbits.

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An Investigation on the Regenerative Effects of Intra Articular Injection of Co-Cultured Adipose Derived Stem Cells with Chondron for Treatment of Induced Osteoarthritis

Cited by 11 publications

References 65 publications

^pNNS-Conjugated Chitosan Mediated IGF-1 and miR-140 Overexpression in Articular Chondrocytes Improves Cartilage Repair

^pNNS-Conjugated Chitosan Mediated IGF-1 and miR-140 Overexpression in Articular Chondrocytes Improves Cartilage Repair

The Effect of Intra-articular versus Intravenous Injection of Mesenchymal Stem Cells on Experimentally-Induced Knee Joint Osteoarthritis

Combination of chondrocytes with chondrons improves Proteoglycan and Collagen-2 production to promote the repairs of defective knee cartilage in rabbits

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