An isotope-labeled chemical derivatization method for the quantitation of short-chain fatty acids in human feces by liquid chromatography–tandem mass spectrometry

Han, Jun; Lin, Karen; Sequeira, Carita; Borchers, Christoph H.

doi:10.1016/j.aca.2014.11.015

Cited by 423 publications

(344 citation statements)

References 54 publications

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“…Cecal SCFAs were determined using ultra‐performance liquid chromatography coupled to tandem mass spectrometry . Approximately 100‐mg cecal content was homogenized in 1 mL 50% aqueous acetonitrile and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

Beneficial effect of butyrate‐producing Lachnospiraceae on stress‐induced visceral hypersensitivity in rats

Zhang

Song

Wang

et al. 2018

J of Gastro and Hepatol

207

157

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Background and Aim Emerging evidence indicates that psychological stress is involved in the pathogenesis of irritable bowel syndrome, which is characterized by visceral hypersensitivity and may be accompanied by gut dysbiosis. However, how such stress contributes to the development of visceral hypersensitivity is incompletely understood. Here, we aimed to investigate the influence that stress‐induced microbial changes exert on visceral sensitivity, as well as the possible underlying mechanisms associated with this effect. Methods Male Sprague–Dawley rats underwent chronic water avoidance stress (WAS) to induce visceral hypersensitivity. Visceral sensitivity, colonic tight junction protein expression, and short‐chain fatty acids of cecal contents were measured. Fecal samples were collected to characterize microbiota profiles. In a separate study, oral gavage of Roseburia in WAS rats was conducted to verify its potential role in the effectiveness on visceral hypersensitivity. Results Repeated WAS caused visceral hypersensitivity, altered fecal microbiota composition and function, and decreased occludin expression in the colon. Stressed rats exhibited reduced representation of pathways involved in the metabolism of butyrate and reduced abundance of several operational taxonomic units associated with butyrate‐producing bacteria, such as Lachnospiraceae. Consistently, supplementation with Roseburia hominis, a species belonging to Lachnospiraceae, significantly increased cecal butyrate content. Moreover, Roseburia supplementation alleviated visceral hypersensitivity and prevented the decreased expression of occludin. Conclusions Reduction in the abundance of butyrate‐producing Lachnospiraceae, which is beneficial for the intestinal barrier, was involved in the formation of visceral hypersensitivity. R. hominis is a potential probiotic for treating stress‐induced visceral hypersensitivity.

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Section: Methodsmentioning

confidence: 99%

Beneficial effect of butyrate‐producing Lachnospiraceae on stress‐induced visceral hypersensitivity in rats

Zhang

Song

Wang

et al. 2018

J of Gastro and Hepatol

207

157

View full text Add to dashboard Cite

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“…Derivatization of the carboxylic compounds in both the technical mixtures and the OSPW extracts was carried out using 3‐NPH as the derivatizing reagent according to a protocol for the derivatization of short‐chain fatty acids that has been described elsewhere . The reaction follows a mechanism of nucleophilic addition/elimination as depicted in Supporting Information Fig.…”

Section: Methodsmentioning

confidence: 99%

Molecular profiling of naphthenic acids in technical mixtures and oil sands process‐affected water using polar reversed‐phase liquid chromatography–mass spectrometry

Han

Lin

et al. 2016

Electrophoresis

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In this work, a reversed-phase ultra-HPLC (UHPLC) ultrahigh resolution MS (UHRMS) method was evaluated for the comprehensive profiling of NAs containing two O atoms in each molecule (O2NAs; general formula C H O , where n is the number of carbon atoms and z represents hydrogen deficiency). Using a polar cyanopropyl-bonded phase column and negative-ion electrospray ionization mass spectrometric detection at 120,000 FWHM (m/z 400), 187 and 226 O2NA species were found in two naphthenic acid technical mixtures, and 424 and 198 species with molecular formulas corresponding to O2NAs were found in two oil sands process-affected water samples (one from a surface mining operation and the other from a steam-assisted gravity drainage operation), respectively. To our knowledge, these are the highest numbers of molecular compositions of O2NAs that have been profiled thus far in environmental samples. Assignments were based on accurate mass measurements (≤3 ppm) combined with rational molecular formula generation, correlation of chromatographic behavior of O2NA homologues with their elemental compositions, and confirmation with carboxyl group-specific chemical derivatization using 3-nitrophenylhydrazine. Application of this UHPLC-UHRMS method to the quantitation of O2NAs in the surface mining operation-derived water sample showed excellent linearity (R = 0.9999) with external calibration, a linear range of 256-fold in concentration, and quantitation accuracies of 64.9 and 69.4% at two "standard substance" spiking levels.

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“…Han et al. successfully developed a novel method that combined chemical derivatization with UHPLC–MS/MS for the synchronous analysis of ten short‐chain FFAs in human fecal samples . Thus, developing selective and sensitive detection strategies toward FFAs in a complex matrix such as formulas is an issue of great interest.…”

Section: Discussionmentioning

confidence: 99%

Mass spectrometric analysis of free fatty acids in infant milk powders by frozen pretreatment coupled with isotope‐labeling derivatization

Zhou

Leng

Peng

et al. 2016

J of Separation Science

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In combination with frozen pretreatment and carboxyl group derivatization, a novel workflow was developed for the determination of free fatty acids in milk powder. The workflow showed a significantly enhanced performance for comprehensive free fatty acid analysis owing to a highly efficient frozen extraction method. In addition, the advantages of the workflow also involved high sensitivity and great tolerance to a complex matrix. Characteristic fragment ions of derivatization reagents also provide clear evidence for the qualitative analysis of free fatty acids. Fourteen types of free fatty acids in a number of domestic and overseas infant milk powders have been successfully detected. The content of free fatty acids in the different samples was different, which probably indicates the diverse quality of infant milk powder. The workflow is expected to be a pragmatic tool for the analysis of free fatty acids in intricate matrices.

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An isotope-labeled chemical derivatization method for the quantitation of short-chain fatty acids in human feces by liquid chromatography–tandem mass spectrometry

Cited by 423 publications

References 54 publications

Beneficial effect of butyrate‐producing Lachnospiraceae on stress‐induced visceral hypersensitivity in rats

Beneficial effect of butyrate‐producing Lachnospiraceae on stress‐induced visceral hypersensitivity in rats

Molecular profiling of naphthenic acids in technical mixtures and oil sands process‐affected water using polar reversed‐phase liquid chromatography–mass spectrometry

Mass spectrometric analysis of free fatty acids in infant milk powders by frozen pretreatment coupled with isotope‐labeling derivatization

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