An LTC4 binding site in gastric mucosa is shared with glutathione

Pendley, Charles; Pendleton, Robert G.; Herczeg, T

doi:10.1016/0090-6980(89)90086-5

Cited by 4 publications

(1 citation statement)

References 21 publications

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“…During the last years the binding of GSH and GSSG to proteins, specially to enzymes participating in the reduction, oxidation and conjugation processes of glutathiones has been vigorously discussed. The binding of glutathiones to various proteins such as rabbit gastric mucosa (6), human plasma (7), F-crystallins (8), insulin (9), acyl carrier protein in spinach seed (I0), Na+-K+-ATP-ase (ii), and proteins isolated from Tripanosoma cruzi (12) has been demonstrated. The individual amino acid residues accounting for the binding of glutathiones to enzymes have not been elucidated in detail.…”

Section: Introductionmentioning

confidence: 99%

Effect of salts on the interaction of oxidized glutathione with dibasic amino acids

Cserháti

1998

IUBMB Life

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The interaction of oxidized glutathione (GSSG) with dibasic amino acids was studied with charge‐transfer chromatography carried out on unimpregnated cellulose layers in the absence and presence of LiCl, NaCl, KCl, MgCl2 and CaCl2. GSSG decreased in each case the apparent lipophilicity of dibasic amino acids, indicating some type of interaction. Calculations proved that the strength of GSSG ‐ dibasic amino acid interaction decreases with increasing concentration of salts suggesting a hydrophilic character of interaction. It can be assumed that electrostatic forces between the carboxyl group of GSSG and the amino groups of amino acids are involved in the interaction. GSSG binds stronger to arginine than to lysine and ornithine suggesting that arginine is probably the primary binding site in proteins for GSSG.

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Section: Introductionmentioning

confidence: 99%

Effect of salts on the interaction of oxidized glutathione with dibasic amino acids

Cserháti

1998

IUBMB Life

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Characterization of leukotriene C₄ binding sites in the brain of the American bullfrog, Rana catesbeiana

1992

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In this study, specific binding sites for [3H]-LTC4 on membrane preparations from American bullfrog (Rana catesbeiana) brain were characterized. Binding assays were done in the presence of serine (5mM) borate (10 mM) for 30 min at 23 degrees C. Under these conditions, no metabolism of LTC4 to LTD4 occurred. Specific binding of [3H]-LTC4 reached steady state within 10 min, remained constant for 60 min, and was reversible with the addition of 1,000-fold excess unlabelled LTC4. Scatchard analysis of the binding data indicated a single class of binding sites with an estimated Kd of 89.83 nM and Bmax of 43.79 pmol/mg protein. Competition binding studies demonstrated that LTD4 and LTE4 were ineffective in displacing [3H]-LTC4 from its binding site. The Ki for LTC4 was 51 nM. S-decylglutathione, glutathione and hematin had Ki values of 44, 312,602, and 25,576 nM, respectively. The mammalian cysteinyl leukotriene antagonist L-660,711 inhibited specific binding of [3H]-LTC4, with a Ki of 87,149 nM. Guanosine-5'-0-3-thiotriphosphate (GTP gamma S) did not affect specific binding of [3H]-LTC4 indicating that, like mammalian LTC4 receptors, a Gi protein is not involved in the transduction mechanism. The LTC4 binding site in bullfrog brain demonstrates both similarities and differences from its mammalian counterpart.

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Biology and pathophysiology of leukotrienes

Denzlinger

1996

Critical Reviews in Oncology/Hematology

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An LTC4 binding site in gastric mucosa is shared with glutathione

Cited by 4 publications

References 21 publications

Effect of salts on the interaction of oxidized glutathione with dibasic amino acids

Effect of salts on the interaction of oxidized glutathione with dibasic amino acids

Characterization of leukotriene C₄ binding sites in the brain of the American bullfrog, Rana catesbeiana

Biology and pathophysiology of leukotrienes

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An LTC4 binding site in gastric mucosa is shared with glutathione

Cited by 4 publications

References 21 publications

Effect of salts on the interaction of oxidized glutathione with dibasic amino acids

Effect of salts on the interaction of oxidized glutathione with dibasic amino acids

Characterization of leukotriene C4 binding sites in the brain of the American bullfrog, Rana catesbeiana

Biology and pathophysiology of leukotrienes

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Characterization of leukotriene C₄ binding sites in the brain of the American bullfrog, Rana catesbeiana