An o-toluidine method for detection of carbohydrates in protein hydrolysates

Morçöl, Tülin; Velander, William H.

doi:10.1016/0003-2697(91)90311-g

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Sugars Isolated From Polysaccharides and Conjugated Carbo1

Effect Of Fma On Immobilized Hpc4-mab: Hpc Avidity1

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Cited by 10 publications

(2 citation statements)

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“…Hydrolysis is usually done with hydrochloric acid, sulfuric acid, or trifluoroacetic acid at concentrations ranging from 0.5 M to up to several moles per liter, and at elevated temperatures. Detailed procedures are described in the literature (25)(26)(27)(28)(29)(30).…”

Section: Sugars Isolated From Polysaccharides and Conjugated Carbomentioning

confidence: 99%

Carbohydrates

Maloney¹

2003

Handbook of Thin-Layer Chromatography

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Section: Sugars Isolated From Polysaccharides and Conjugated Carbomentioning

confidence: 99%

Carbohydrates

Maloney¹

2003

Handbook of Thin-Layer Chromatography

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“…The immunosorbent applications having no FMA present during immobilization possessed half-maximal inhibition of hPC binding signal by HCPC [6][7][8][9][10][11][12][13][14][15][16][17] at a 0.05 k0.002 molar ratio. The applications having HCPC[6-171 as the FMA possessed halfmaximal inhibition of hPC binding at a molar ratio of HCPC [6][7][8][9][10][11][12][13][14][15][16][17]/hPC ranging from 0.049 to 0.052. The HPC4-Mab applications which were masked with the 1K adduct produced an avidity for hPC similar to that of peptide with half-maximal inhibition occurring at a molar ratio of HCPC[6-17]/HPC4-Mab ranging from 0.053 to 0.060.…”

Section: Effect Of Fma On Immobilized Hpc4-mab: Hpc Aviditymentioning

confidence: 99%

The use of Fab‐masking antigens to enhance the activity of immobilized antibodies

Velander

Subramanian

Madurawe

et al. 1992

Biotech & Bioengineering

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This study demonstrates that masking the Feb regions of a monoclonal antibody (Mab) with synthetic antigens prior to covalent immobilization efficiency. Water-soluble adducts of poly(2-methyloxazoline) polymers and a synthetic-peptide epitope for the Mab were constructed. These synthetic antigens are referred to as Fab-masking antigents (FMAs). The antibody used in this study is a Ca(2+)-dependent murine monoclonal lgG directed against the plasma protein, human protein C (hPC). The FMAs were pre-equilibrated with Mab in the presence of calcium prior to immobilization and were then removed by EDTA, which destabilized the FMA-Mab complexes. The antigen binding efficiency and accessibility of the Fab domain of the immobilized antibody was significantly increased for Mab immobilized in the presence of FMA relative to those Mab immobilized without FMA. The increase in binding efficiency was most pronounced for the largest FMA employed. No appreciable differences were detected in the avidity of hPC-Mab complexes formed by immunosorbents produced by either masked or unmasked antibody. These results provide evidence that orientation may play an important role in the binding activity of immobilized antibodies.

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Carbohydrates

Pomeranz¹,

Meloan²

1994

Food Analysis

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An o-toluidine method for detection of carbohydrates in protein hydrolysates

Cited by 10 publications

References 24 publications

Carbohydrates

Carbohydrates

The use of Fab‐masking antigens to enhance the activity of immobilized antibodies

Carbohydrates

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