2023
DOI: 10.1101/2023.06.05.543771
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An obligate intracellular bacterial pathogen forms a direct, interkingdom membrane contact site

Abstract: Interorganelle communication regulates cellular homeostasis through the formation of tightly-associated membrane contact sites 1,3. Prior work has identified several ways that intracellular pathogens alter contacts between eukaryotic membranes 4,6, but there is no existing evidence for contact sites spanning eukaryotic and prokaryotic membranes. Here, using a combination of live-cell microscopy and transmission and focused-ion-beam scanning electron microscopy, we demonstrate that the intracellular bacterial p… Show more

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Cited by 3 publications
(1 citation statement)
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References 49 publications
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“…N- terminally tagged SrfB, SrfC, and SrfE expressed poorly, so pRL0383, pRL0384, and pRL0386 respectively contain a C-terminal GGSGS DYKDHDGDYKDHDIDYKDDDDK sequence instead. FCW2IB-BiP-mNeonGreen-KDEL was generated as previously described 74 . pRL0389 was made by replacing the Lifeact-3xTagBFP insert in FCW2IB- Lifeact-3xTagBFP 4 with Gaussia -Dura luciferase from pCMV-Gaussia-Dura Luc (Thermo Fisher Scientific catalog number 16191).…”
Section: Methodsmentioning
confidence: 99%
“…N- terminally tagged SrfB, SrfC, and SrfE expressed poorly, so pRL0383, pRL0384, and pRL0386 respectively contain a C-terminal GGSGS DYKDHDGDYKDHDIDYKDDDDK sequence instead. FCW2IB-BiP-mNeonGreen-KDEL was generated as previously described 74 . pRL0389 was made by replacing the Lifeact-3xTagBFP insert in FCW2IB- Lifeact-3xTagBFP 4 with Gaussia -Dura luciferase from pCMV-Gaussia-Dura Luc (Thermo Fisher Scientific catalog number 16191).…”
Section: Methodsmentioning
confidence: 99%