An odorant binding protein is involved in counteracting detection-avoidance and Toll-pathway innate immunity

Zhang, Wei; Xie, Mushan; Eleftherianos, Ioannis; Mohamed, Amr A.; Cao, Yueqing; Song, Baoan; Zang, Lian‐Sheng; Chen, Jia; Bian, Jing; Keyhani, O. Nemat; Xia, Yuxian

doi:10.1016/j.jare.2022.08.013

Cited by 22 publications

(17 citation statements)

References 77 publications

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“…cerana immune response after NC1 and NF inoculation at 13 d.p.i., especially OBP13 (Table ). OBPs, water-soluble proteins, are responsible for the delivery of volatile organic compounds to the odorant receptors , but more recently, OBPs have been discovered in non-sensory organs and thus associated with other physiological processes. − Similar to ferritins, OBPs also were mentioned to be involved in the immune responses in Glossina spp, for example. In 2022, Zhang and collaborators demonstrated the relationship between OBPs and Toll pathway innate immunity in locusts.…”

Section: Resultsmentioning

confidence: 99%

“…be involved in the immune responses in Glossina spp, 76 for example. In 2022, Zhang and collaborators 74 demonstrated the relationship between OBPs and Toll pathway innate immunity in locusts. In A. mellifera and A. cerana, 21 and 17 OBPs, respectively, were identified that are associated with the olfactory system and physiological functions.…”

Section: Off-gel Bottom-up Proteomics Analysis Of the Honeybee Hemoly...mentioning

confidence: 99%

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Proteomics and Immune Response Differences in Apis mellifera and Apis cerana Inoculated with Three Nosema ceranae Isolates

et al. 2023

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Nosema ceranae infects midgut epithelial cells of the Apis species and has jumped from its original host A. cerana to A. mellifera worldwide, raising questions about the response of the new host. We compared the responses of these two species to N. ceranae isolates from A. cerana, A. mellifera from Thailand and A. mellifera from France. Proteomics and transcriptomics results were combined to better understand the impact on the immunity of the two species. This is the first combination of omics analyses to evaluate the impact of N. ceranae spores from different origins and provides new insights into the differential immune responses in honeybees inoculated with N. ceranae from original A. cerana. No difference in the antimicrobial peptides (AMPs) was observed in A. mellifera, whereas these peptides were altered in A. cerana compared to controls. Inoculation of A. mellifera or A. cerana with N. ceranae upregulated AMP genes and cellular-mediated immune genes but did not significantly alter apoptosis-related gene expression. A. cerana showed a stronger immune response than A. mellifera after inoculation with different N. ceranae isolates. N. ceranae from A. cerana had a strong negative impact on the health of A. mellifera and A. cerana compared to other Nosema isolates.

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Section: Resultsmentioning

confidence: 99%

Section: Off-gel Bottom-up Proteomics Analysis Of the Honeybee Hemoly...mentioning

confidence: 99%

Proteomics and Immune Response Differences in Apis mellifera and Apis cerana Inoculated with Three Nosema ceranae Isolates

et al. 2023

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“…aegypti mosquitoes . In addition, the entomopathogenic fungus Metarhizium anisopliae produces phenylethyl alcohol that induces behavioral avoidance of Locusta migratoria . Therefore, the use of semiochemicals released by herbivore-associated microbes to regulate insect behavior and monitor pest populations is a potential novel strategy for pest management.…”

Section: Resultsmentioning

confidence: 99%

Semiochemicals Produced by Microbes in Mealybug Honeydew Attract Fire Ants

Zhang,

Fu,

Shao

et al. 2023

J. Agric. Food Chem.

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Semiochemicals produced by microbes in hemipteran honeydew play an important role in mediating the ant–hemipteran interaction. However, the specific volatile compounds and their origins remain unclear. Here, we found that honeydew produced by Phenacoccus solenopsis exhibited strong attractiveness to fire ant workers, but sterilized honeydew was much less attractive. Four compounds were identified from the honeydew volatiles, including 1-octen-3-ol, limonene, phenylethyl alcohol, and 2,4-ditert-butylphenol. All the identified compounds triggered strong electroantennography response of fire ants and showed repellence at relatively high concentrations while attractiveness at low concentrations to fire ant workers. Furthermore, six bacterial isolates were identified based on 16S rRNA sequencing, namely, Bacillus, Brachybacterium, Kocuria, Microbacterium, Pseudomonas, and Staphylococcus. Fire ants exhibited a strong preference for Brachybacterium, Kocuria, and Microbacterium, suggesting that these bacterial isolates are involved in the attracting effect of honeydew. Both limonene and phenylethyl alcohol were detected from Brachybacterium, while limonene was only detected from Kocuria, and phenylethyl alcohol was exclusively detected from Microbacterium. Reinoculation of these bacteria restored the attractiveness of honeydew to fire ants, and the active compounds, limonene and phenylethyl alcohol, were detectable in bacteria-reinoculated honeydew. Collectively, our results reveal the active compounds in hemipteran honeydew and their association with honeydew bacteria. The findings will contribute to the development of novel attractants for efficient monitoring of fire ants.

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“…The dissociation constant (K i ) of 1-NPN by competing ligands was calculated with the following formula using IC50 value: K i = [IC50]/(1 + [1-NPN]/K 1-NPN ). Here, IC50 is the concentration of competing ligands when the fluorescence intensity reaches half of the initial fluorescence intensity of 1-NPN, and [1-NPN] indicates the concentration of 1-NPN [ 16 ]. A threshold of K i < 20 µM was recognized as binding.…”

Section: Methodsmentioning

confidence: 99%

“…However, other studies have demonstrated that the expression of odorant-binding proteins in other tissues such as the salivary glands, fat body, or midgut may be associated with alternative physiological functions in the insect [ 11 , 12 , 13 ]. Some OBPs , for instance, have been found to relate to innate immunity, pesticide resistance, and anti-inflammation [ 14 , 15 , 16 ]. The expression of CSPs has been reported in diverse insect tissues [ 17 , 18 ] to function in pheromone delivery, nutrient transportation, visual pigmentation, development, immunity, or pesticide resistance [ 19 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Odorant-Binding Proteins and Chemosensory Proteins in Spodoptera frugiperda: From Genome-Wide Identification and Developmental Stage-Related Expression Analysis to the Perception of Host Plant Odors, Sex Pheromones, and Insecticides

Jia

Mohamed

Cattaneo

et al. 2023

IJMS

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Spodoptera frugiperda is a worldwide generalist pest with remarkable adaptations to environments and stresses, including developmental stage-related behavioral and physiological adaptations, such as diverse feeding preferences, mate seeking, and pesticide resistance. Insects’ odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are essential for the chemical recognition during behavioral responses or other physiological processes. The genome-wide identification and the gene expression patterns of all these identified OBPs and CSPs across developmental stage-related S. frugiperda have not been reported. Here, we screened for genome-wide SfruOBPs and SfruCSPs, and analyzed the gene expression patterns of SfruOBPs and SfruCSPs repertoires across all developmental stages and sexes. We found 33 OBPs and 22 CSPs in the S. frugiperda genome. The majority of the SfruOBP genes were most highly expressed in the adult male or female stages, while more SfruCSP genes were highly expressed in the larval or egg stages, indicating their function complementation. The gene expression patterns of SfruOBPs and SfruCSPs revealed strong correlations with their respective phylogenic trees, indicating a correlation between function and evolution. In addition, we analyzed the chemical-competitive binding of a widely expressed protein, SfruOBP31, to host plant odorants, sex pheromones, and insecticides. Further ligands binding assay revealed a broad functional related binding spectrum of SfruOBP31 to host plant odorants, sex pheromones, and insecticides, suggesting its potential function in food, mate seeking, and pesticide resistance. These results provide guidance for future research on the development of behavioral regulators of S. frugiperda or other environmentally friendly pest-control strategies.

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An odorant binding protein is involved in counteracting detection-avoidance and Toll-pathway innate immunity

Cited by 22 publications

References 77 publications

Proteomics and Immune Response Differences in Apis mellifera and Apis cerana Inoculated with Three Nosema ceranae Isolates

Proteomics and Immune Response Differences in Apis mellifera and Apis cerana Inoculated with Three Nosema ceranae Isolates

Semiochemicals Produced by Microbes in Mealybug Honeydew Attract Fire Ants

Odorant-Binding Proteins and Chemosensory Proteins in Spodoptera frugiperda: From Genome-Wide Identification and Developmental Stage-Related Expression Analysis to the Perception of Host Plant Odors, Sex Pheromones, and Insecticides

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