An on-line detection system for screening small molecule inhibitors of α-Amylase and α-Glucosidase in Prunus mume

Nan, Xiaoke; Jia, Weijuan; Zhang, Yuankuan; Wang, Hong; Lin, Zongtao; Chen, Shizhong

doi:10.1016/j.chroma.2021.462754

Cited by 18 publications

(11 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Isomers of the compounds assigned in this study as acetyl- p -coumaroylsucroses ( peaks 3 , 11 – 19 , and 22 – 26 ) ( Table 2 ) have already been identified in Prunus mume flowers and fruits [ 29 , 41 , 42 , 66 , 67 , 68 , 69 , 70 , 71 , 72 ], although, to date, none of those addresses their quantification, preventing us from making a comparison with the amounts obtained for Prunus lusitanica .…”

Section: Resultsmentioning

confidence: 94%

“…Peak 1 ([M−H] − at m / z 353) was identified as 3- O -caffeoylquinic acid, yielding the base peak at m / z 191, corresponding to deprotonated quinic acid, [quinic acid−H] − , and another characteristic ion at m / z 179, [caffeic acid−H] − , in MS 2 [ 27 , 28 , 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…Peak 21 presented an [M−H] − ion at m / z 609 and the characteristic ion corresponding to the quercetin aglycone at m / z 301, derived from the loss of a molecule of rhamnose and the successive loss of a molecule of glucose [M−H−146−162] − . In this sense, compounds 20 and 21 were identified as quercetin-3- O -glucoside and quercetin-3- O -rutinoside, respectively [ 29 , 46 ].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Prunus lusitanica L. Fruits as a Novel Source of Bioactive Compounds with Antioxidant Potential: Exploring the Unknown

et al. 2022

View full text Add to dashboard Cite

Prunus lusitanica L., also known as Portuguese laurel or locally known as ‘azereiro’, is a rare species with ornamental and ecological value. Only two studies regarding the bioactivity and chemical composition of its leaves were reported to date. Thus, the present study aims to qualitatively and quantitatively evaluate the phenolic profile, through HPLC-PAD-ESI-MS/MS (high-performance liquid chromatography–photodiode array detection–electrospray ionization tandem mass spectrometry), as well as the radical scavenging capacity, through ABTS (2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1 picrylhydrazyl), and the reducing power (FRAP, ferric reducing antioxidant power) assays, of P. lusitanica fruits during a 4-year study. In total, 28 compounds were identified and quantified in the fruits, including 21 hydroxycinnamic acids (60.3%); 2 flavan-3-ols (27.9%), 2 anthocyanins (10.5%), 2 flavonols (1.0%), and 1 secoiridoid (0.3%). High antioxidant capacity was observed, with ABTS values ranging from 7.88 to 10.69 mmol TE (Trolox equivalents)/100 g fw (fresh weight), DPPH values from 5.18 to 8.17 mmol TE/100 g fw, and FRAP values from 8.76 to 11.76 mmol TE/100 g fw. According to these results, it can be concluded that these are rich sources of phenolic compounds with very promising antioxidant capacity and, therefore, with potential applications in the food and/or phytopharmaceutical sectors.

show abstract

Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Prunus lusitanica L. Fruits as a Novel Source of Bioactive Compounds with Antioxidant Potential: Exploring the Unknown

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In SQE, the quencher and fluorophore combine via hydrogen bonding, hydrophobic interaction, and electrostatic attraction to form a complex with no luminescence or weak luminescence intensity, causing the fluorescence quenching, and the static quenching constant declines with the rise of temperature. 51 Thus, the Stern–Volmer quenching constant at different temperatures was investigated according to eqn (2) 50,52 where [Q] is the concentration of DCIP; F 0 and F are the luminescence intensities of Ce@AuNCs without and with DCIP, respectively. K SV and K q are the quenching constant and fluorescence quenching rate constant, respectively.…”

Section: Resultsmentioning

confidence: 99%

Section: Characterization Of Auncs and Ce@auncsmentioning

confidence: 99%

Fluorometric and colorimetric dual-mode sensing of α-glucosidase based on aggregation-induced emission enhancement of AuNCs

Li,

Zhang,

Huang

et al. 2024

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

Interaction of narcissoside with α‐amylase from Bacillus subtilis and Porcine pancreatic by multi‐spectral analysis and molecular dynamics simulation

Cui,

Fan,

Lian

et al. 2023

Luminescence

View full text Add to dashboard Cite

In this work, interaction mechanism of narcissoside with two α‐amylase from Bacillus subtilis (BSA) and Porcine pancreatic (PPA) are comparatively studied by multi‐spectral analysis, molecular docking and molecular dynamics simulation. The results prove that narcissoside can statically quench fluorescence of BSA/PPA. Two complexes are mainly formed by hydrogen bond and van der Waals force. With the increase of temperature, the two complexes formed by narcissoside and two enzymes become unstable. At the same experimental temperature, the binding force of narcissoside to PPA is higher than that of BSA. The binding of narcissoside to PPA/BSA increases the hydrophobicity of microenvironment. Moreover, the secondary structure of PPA/BSA is mainly changed by decreasing the α‐helix. The optimal binding modes of narcissoside with BSA/PPA are predicted by molecular docking, and the stability of the two complexes is evaluated by molecular dynamics simulations.

show abstract

An on-line detection system for screening small molecule inhibitors of α-Amylase and α-Glucosidase in Prunus mume

Cited by 18 publications

References 32 publications

Prunus lusitanica L. Fruits as a Novel Source of Bioactive Compounds with Antioxidant Potential: Exploring the Unknown

Prunus lusitanica L. Fruits as a Novel Source of Bioactive Compounds with Antioxidant Potential: Exploring the Unknown

Fluorometric and colorimetric dual-mode sensing of α-glucosidase based on aggregation-induced emission enhancement of AuNCs

Interaction of narcissoside with α‐amylase from Bacillus subtilis and Porcine pancreatic by multi‐spectral analysis and molecular dynamics simulation

Contact Info

Product

Resources

About