An open gas-exchange system for the simultaneous measurement of the CO2 and 14CO2 fluxes from leaves

Ludwig, L. J.; Canvin, David T.

doi:10.1139/b71-185

Cited by 77 publications

(43 citation statements)

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“…the precursor for sorbitol (10,18,20), is an activator of sucrose-phosphate synthetase (24), a key enzyme of the sucrose pathway. (14). Moreover, a preliminary experiment (I showed that the leaf blade thickness of fixe4 mature leaves was between 0.14 and 0.18 we can assume that the Geiger-Muller tub( activity in the whole thickness of the leaf redistribution of radioactivity among indivi had created little artifact.…”

Section: Partitioning Of Photosynthetic Carbonmentioning

confidence: 94%

Carbon Fluxes in Mature Peach Leaves

Moing¹,

Carbonne²,

Rashad³

et al. 1992

Plant Physiol.

107

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The turnover and transport of sugars are described in peach (Prunus persica L. Batsch), a species exporting both sucrose and sorbitol. Apparent export rate was slower in peach leaves than in leaves of herbaceous species. Sorbitol was the major soluble end product of photosynthesis and the major soluble carbohydrate in the leaf (higher than sucrose). Carbon fluxes were described using 14C labeling, radioactivity loss curves, and compartmental analysis during the second half of the photoperiod when chemical steady state was reached for soluble carbohydrates. The measured specific radioactivity of sucrose was typical of a primary product. The delayed decrease in specific radioactivity of sorbitol indicated that part of it was secondarily synthesized. Sucrose is proposed to be the carbon source for the delayed synthesis of sorbitol in the light. The sorbitol to sucrose ratio was higher in the petiole than in the leaf tissues. In phloem sap, obtained using stylectomy of aphids and collected from the main stem between source leaves and apex, this ratio was lower than in the petiole, suggesting a preferential sorbitol demand by sinks.

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Section: Partitioning Of Photosynthetic Carbonmentioning

confidence: 94%

Carbon Fluxes in Mature Peach Leaves

Moing¹,

Carbonne²,

Rashad³

et al. 1992

Plant Physiol.

107

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“…Photosynthesis was calculated based on the 14 CO2 fixed during 30 s exposures and our estimates thus represent values closer to gross, rather than net, photosynthesis. 6 Details of these methods and the calculations were described previously. 5 Needle water potential (measured by pressure chamber) averaged -11.4 bars during the season and ranged between -14 and -8 bars.…”

Section: Experimental Methodsmentioning

confidence: 99%

Comparative Ozone Dose Response of Gas Exchange in a Ponderosa Pine Stand Exposed to Long-Term Fumigations

Coyne

Bingham

1981

Journal of the Air Pollution Control Association

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“…A cylinder (4-0 dm 3 ) containing compressed dry air and 14 CO 2 was prepared as described by Ludwig and Canvin (1970). Ten cm 3 air with a known amount of I4 CO 2 was taken from the cylinder using a syringe and, after the system was closed, injected into the cuvette where it was rapidly distributed by the fan.…”

Section: Gas Exchange Analysis With Intact Leavesmentioning

confidence: 99%

Influence of Temperature on the Ratio of Ribulose Bisphosphate Carboxylase to Oxygenase Activities and on the Ratio of Photosynthesis to Photorespiration of Leaves

Lehnherr¹,

Mächler²,

Nösberger³

1985

J Exp Bot

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Lehnherr, B., Machler, F. and Nosberger, J. 1985. Influence of temperature on the ratio of ribulose bisphosphate carboxylase to oxygenase activities and on the ratio of photosynthesis to photorespiration of leaves.-J. exp. Bot. 36: 1117-1125.Rates of net and gross photosynthesis of intact white clover leaves were measured by infrared gas analysis and by short term uptake of 14 CO 2 respectively. Ribulose bisphosphate carboxylase oxygenase (RuBPCO) was purified from young leaves and kinetic properties investigated in combined and separate assays. The ratio of carboxylase to oxygenase activities was compared with the ratio of photosynthesis to photorespiration at various temperatures and CO 2 concentrations.The ratio of photosynthesis to photorespiration at 30 Pa p(CO 2 ) was consistent with the ratio of carboxylase activity to oxygenase activity when each was measured above 20 °C. However, the ratio of photosynthesis to photorespiration increased with decreasing temperature, whereas the ratio of carboxylase to oxygenase activity was independent of temperature. This resulted in a disagreement between the measurements on the purified enzyme and intact leaf at low temperature. No disagreement between enzyme and leaf at low temperature occurred, when the ratio of photosynthesis to photorespiration was determined at increased CO 2 concentrations.The results suggest an effect of low temperature and low CO 2 concentration on the ratio of photosynthesis to photorespiration independent of the enzyme.

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An open gas-exchange system for the simultaneous measurement of the CO₂ and ¹⁴CO₂ fluxes from leaves

Cited by 77 publications

References 0 publications

Carbon Fluxes in Mature Peach Leaves

Carbon Fluxes in Mature Peach Leaves

Comparative Ozone Dose Response of Gas Exchange in a Ponderosa Pine Stand Exposed to Long-Term Fumigations

Influence of Temperature on the Ratio of Ribulose Bisphosphate Carboxylase to Oxygenase Activities and on the Ratio of Photosynthesis to Photorespiration of Leaves

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