An Open-Source 3D–Printed Recording Stage with Customizable Chambers for Ex Vivo Experiments

Abstract

Much of what has been discovered concerning neurophysiological mechanisms can be credited to ex vivo biomedical experiments. Beyond these discoveries, ex vivo research techniques have enhanced the global understanding of human physiology and pathology in almost every biomedical specialty. Naturally, ex vivo experiments are among the most desired methods of research, particularly in the field of neuroscience. Ex vivo experiment platforms may be purchased commercially. However, their substantial cost and sometimes limited availability can render them inaccessible to many research labs. Moreover, these manufactured systems are often rigid in function with no possibility of customization, severely narrowing their capabilities. However, developing essential components for ex vivo laboratory systems with a fused deposition modeling (FDM) printer provides a practical solution to each of these obstacles. Here, we provide the designs and construction process for an easily accessible, highly adaptable recording stage with modifiable submersion chambers using a 3D printer for a total cost under $15.00. With the versatility afforded by the exchangeable custom chambers, the system may be used to conduct research on a variety of ex vivo tissue preparations, paving the way for novel research.Significance statementEx vivo research techniques on animal models are critical to ongoing research in most medical fields, and much of what has been discovered concerning the physiology of neurons can be attributed to such experiments. As useful as commercially available designs have proven to be, they can prove difficult to access for some researchers due to their significant costs. Moreover, these systems are often restricted in their experimental capabilities and are unable to be modified. We propose a 3D-printable design that is readily available, incredibly affordable, highly adaptable, and completely customizable, a design capable of moving crucial research forward in meaningful ways.