An open-source smart fraction collector for isocratic preparative liquid chromatography

Medina, Deyber Arley Vargas; Lozada-Blanco, Asdrubal; Rodríguez, Julie Paulin García; Lanças, Fernando Mauro; Santos-Neto, Álvaro José

doi:10.1016/j.ohx.2023.e00462

Cited by 5 publications

(4 citation statements)

References 19 publications

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“…This compound has also been identified in experiments using equivalent working methods, including Preparative HPLC separation and isolating equipment with different specifications and columns, including different methanol-water mobile phases and eluent fractions (Medina et al. 2023 ). The separation and isolation device used in a previous study employed semipreparative HPLC with an analytical column.…”

Section: Resultsmentioning

confidence: 99%

Efficacy of quercetin-like compounds from the mistletoe plant of Dendrophthoe pentandra L. Miq, as oral random blood sugar lowering treatment in diabetic rats

Lazuardi,

Anjani,

Budiatin

et al. 2024

Veterinary Quarterly

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Section: Resultsmentioning

confidence: 99%

Efficacy of quercetin-like compounds from the mistletoe plant of Dendrophthoe pentandra L. Miq, as oral random blood sugar lowering treatment in diabetic rats

Lazuardi,

Anjani,

Budiatin

et al. 2024

Veterinary Quarterly

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“…An Arduino Due was used to control a similar platform for MALDI spotting following a CE separation [51]. Multiple other fraction collectors with minor variations but similar implementation of microcontrollers for motor control have been reported for analytical [52] and preparative [53][54][55] LC separations (or similar volume scale fraction collection from samples prior to LC analysis). Motor control for a system designed for direct surface sampling from a thin-layer chromatography (TLC) plate to an LC-MS system [56] was adapted from a similar, previously reported method for interfacing TLC directly to MS [57].…”

Section: Autosamplers Valving and Other System Controlmentioning

confidence: 99%

The utilization of open‐source microcontroller boards and single‐board computers in liquid chromatography

Piccolo,

Foster,

Parker

et al. 2024

J of Separation Science

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Electronic system control of analytical instrumentation remains a critical aspect of modern measurement science. Within the field of liquid chromatography (LC), this is especially relevant for automation, module operation, detection, data acquisition, and data analysis. Increasingly, home‐built analytical tools used for liquid‐phase separations rely upon open‐source microcontrollers and single‐board computers to aid in simplifying these operations. In this review, we detail literature reported within the past 5 years in which these types of devices were used to advance various aspects of the LC research field, including sample preparation, instrument control, and data collection.

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“…Despite their simplicity, commercial fraction collectors are arguably overpriced, as is most commercial scientific equipment [25][26][27]. This has prompted scientists to build their own fraction collectors [28][29][30][31]. Adapting these designs, or some autosampler designs [32], could be an option for our purposes.…”

Section: Design 21 Justification Of the Designmentioning

confidence: 99%

“…A better solution would be to have the HPLC equipped with a sensor, so that tasks like optimization of retention time determination and peak separation could be performed without requiring EA-IRMS. By employing sensors, "smart" fraction collection could also be attempted, allowing for even further optimization [31].…”

Section: Supplementary Materialsmentioning

confidence: 99%

Turning a 3D Printer into a HPLC Fraction Collector: A Tool for Compound-Specific Stable Isotope Measurements

Carvalho,

Oakes

2023

Hardware

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Compound-specific isotope analysis (CSIA) can provide unique insights into the cycling of elements including carbon and nitrogen. One approach for CSIA is the use of high-performance liquid chromatography (HPLC) to separate compounds of interest, followed by analysis of these compounds using an elemental analyser coupled to an isotope ratio mass spectrometer. A key component of this technique is the fraction collector, which automatically collects compounds as they are separated by HPLC. Here, we present a fraction collector that is a simple adaptation of a 3D printer, and, thus, can be easily adopted by any laboratory already equipped for HPLC. In addition to the much lower cost compared to commercial alternatives, this adaptation has the advantage for CSIA that the 3D printer is able to heat the collected fractions, which is not true for many commercial fraction collectors. Heating allows faster evaporation of the solvent, so that the dried compounds can be measured by EA–IRMS immediately. The procedure can be repeated consecutively so that diluted solutions can have the compounds concentrated for analysis. Any computer-controlled HPLC can be integrated to the fraction collector used here by means of AutoIt.

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An open-source smart fraction collector for isocratic preparative liquid chromatography

Cited by 5 publications

References 19 publications

Efficacy of quercetin-like compounds from the mistletoe plant of Dendrophthoe pentandra L. Miq, as oral random blood sugar lowering treatment in diabetic rats

Efficacy of quercetin-like compounds from the mistletoe plant of Dendrophthoe pentandra L. Miq, as oral random blood sugar lowering treatment in diabetic rats

The utilization of open‐source microcontroller boards and single‐board computers in liquid chromatography

Turning a 3D Printer into a HPLC Fraction Collector: A Tool for Compound-Specific Stable Isotope Measurements

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