An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

Anwar, Firoz; Sharmila, P.; Saradhi, P. Pardha

doi:10.1007/s12298-008-0031-1

Cited by 20 publications

(15 citation statements)

References 40 publications

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“…All the other substrates were inferior for acclimatization of the plantlets, due to the high or low water-retaining capacity of the substrates. The present results demonstrated the importance of the substrate characteristics used during acclimatization, in agreement with Anwar et al (2008).…”

Section: Discussionsupporting

confidence: 90%

Preconditioning effect of cytokinins on in vitro multiplication of embryonic node of grass pea (Lathyrus sativus L.) cultivar Gürbüz

Barpete¹,

Aasım²,

Khawar³

et al. 2014

Turk J Biol

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Lathyrus sativus L. (grass pea) is an important grain legume crop grown all over the world. It is very recalcitrant and difficult\ud to regenerate under in vitro conditions. The present study determined the effect of preconditioning of cytokinins [10 and 20 mg L–1\ud thidiazuron (TDZ) and 2-isopentenyl adenine (2iP)] and postconditioning of dissimilar levels of TDZ-indole-3-butyric acid (IBA)\ud and 2iP-IBA on in vitro shoot multiplication using embryonic node explants. The results showed that preconditioning followed by\ud postconditioning on Murashige and Skoog (MS) medium with 0.25, 0.50, 0.75, and 1.0 mg L–1 2iP or TDZ combined with 0.10 mg\ud L–1 IBA had positive effects on shoot multiplication. It seemed as if the TDZ-preconditioned and TDZ-IBA–postconditioned explants\ud were more regenerative compared to 2iP-preconditioned or 2iP-IBA–postconditioned explants. Maximum shoot induction (100%) was\ud achieved on MS medium containing 1 mg L–1 TDZ and 0.10 mg L–1 IBA, with a mean number of 31.10 shoots per explant. In comparison\ud to the other cytokinin tested, 2iP-preconditioned and 2iP-IBA–postconditioned explants had longer shoots, with a mean shoot length of\ud 8.10 cm. Root initiation was observed on all cultures 4 weeks after the transfer of shoots with the best rooting on MS medium containing\ud 2 mg L–1 IBA. The rooting response was 76.56% with a mean number of 8.03 roots per shoot and root length of 8.2 cm. The healthy\ud plants were transferred to a greenhouse for acclimatization and exhibited 90% survival. It was concluded that TDZ preconditioning was\ud necessary for grass pea multiplication along with TDZ-IBA postconditioning. This regeneration protocol may facilitate future genetic\ud transformation and breeding studies to reduce β-N-oxalyl-L-α,β-di-amino propionic acid content in grass pea

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Section: Discussionsupporting

confidence: 90%

Preconditioning effect of cytokinins on in vitro multiplication of embryonic node of grass pea (Lathyrus sativus L.) cultivar Gürbüz

Barpete¹,

Aasım²,

Khawar³

et al. 2014

Turk J Biol

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“…The highest response was recorded with 2 mg L -1 TDZ + 0.1 mg L -1 24-Epi. These findings are similar to those of Anwar et al (2008 and and Jiang et al (2005). Concerning regeneration from root explants, it is observed that root explants completely failed to shootlets regeneration.…”

Section: Culture Media Composition Rooting (%) Number Of Roots Root Lsupporting

confidence: 86%

“…Furthermore, TDZ may be involved in the synthesis and/or accumulation of cytokinins in plant tissue culture (Carvalho et al, 2000). In this regard, TDZ is considered one of the most promising cytokinin for shoot induction in legumes (Jayanand et al, 2003;Tewari et al, 2004;Kiran et al, 2005;Anwar et al, 2008Anwar et al, , 2010. TDZ was most effective inducing healthier shoots when used at lower concentration (~2.2 mg L -1 ) compared to higher concentrations (Jiang et al, 2005;Anwar et al, 2008Anwar et al, , 2010.…”

Section: Culture Media Composition Rooting (%) Number Of Roots Root Lmentioning

confidence: 99%

Influence of 24- epibrassinolide on in vitro shootlets regeneration via direct organogenesis of Phaseolus vulgaris L.

Taha¹,

Nafie²,

El-Bahr³

et al. 2014

Afr. J. Biotechnol.

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This study aimed to optimize an in vitro shootlets regeneration system via direct organogenesis for Phaseolus vulgaris L. cv. Brunca using 24-epibrassinolide (24-Epi). The best medium for shootlets proliferation as well as growth parameters was MS supplemented with 2 mg L-1 TDZ + 0.1 mg L-1 24-Epi. Stem explants recorded the best results of shootlets proliferation percentage compared with leaf or root explants, respectively. MS medium supplemented with 2 mg L-1 TDZ + 0.1 mg L-1 24-Epi gave the best results of recovered shootlets (%); photosynthetic pigments, number of leaves, number of shootlets and shootlet length (cm). From the obtained results it can be recognized that, the behavior and trends of 24-Epi on in vitro P. vulgaris cultures media may be oriented and arranged to the behavior of auxins in the nutrient medium for roots formation and as a cytokinin in shootlets proliferation.

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“…Many studies have reported the effective elongation of in vitro shoots by the GA 3 in the medium (Anwar et al 2008). On the other hand, some other studies find transfer of shoots to low PGR (lower to the level on which shoot multiplication achieved) medium or PGRfree medium as a successful way for elongation of shoots (Sivanesan and Jeong 2010).…”

Section: Discussionmentioning

confidence: 99%

Enhanced shoot multiplication in Ficus religiosa L. in the presence of adenine sulphate, glutamine and phloroglucinol

Siwach

Gill

2011

Physiol Mol Biol Plants

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Ficus religiosa (Pipal) is a long-lived valuable multipurpose forest tree. The tree is exploited because of its religious, ornamental and medicinal value and the regeneration rate in natural habitat is low. An in vitro propagation protocol has been developed from nodal segments obtained from a 45-50-year old tree. The highest bud break frequency (100 %) followed by maximum number of multiple shoots (13.9) as well as length (2.47 cm) were obtained on Woody Plant medium (WPM) supplemented with 1.0 mg/l BAP along with 0.5 mg/l IAA. Two modifications in this medium resulted in enhanced shoot regeneration-one with 200 mg/l glutamine + 150 mg/l ADS (called as MM-1) giving 32.5 shoots per nodal explant while another modification-with 200 mg/l glutamine + 150 mg/l ADS + 100 mg/l phloroglucinol (called as MM-2) giving 35.65 shoots per explant. These two media were used for sub-culturing of shoots for 4 months. The rate of shoot multiplication was same during the first three sub-cultures on MM-1 and the shoots regenerated were healthy, afterwards shoot multiplication declined. While on MM-2, shoot multiplication declined after first sub-culture and shoots underwent the problem of early leaf fall. Rooting was best induced in micro-shoots excised from proliferated shoot cultures on semi-solid as well as liquid WPM modified with 2.0 mg/l IBA and 0.5 mg/l IAA. The in vitro-raised plantlets were potted and acclimatized under culture room conditions for 25-30 days before transfer to soil conditions, where the established plants showed more than 90 % survival.

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An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

Cited by 20 publications

References 40 publications

Preconditioning effect of cytokinins on in vitro multiplication of embryonic node of grass pea (Lathyrus sativus L.) cultivar Gürbüz

Preconditioning effect of cytokinins on in vitro multiplication of embryonic node of grass pea (Lathyrus sativus L.) cultivar Gürbüz

Influence of 24- epibrassinolide on in vitro shootlets regeneration via direct organogenesis of Phaseolus vulgaris L.

Enhanced shoot multiplication in Ficus religiosa L. in the presence of adenine sulphate, glutamine and phloroglucinol

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