An Optimised Direct Lysis Method for Viral RNA Extraction and Detection of Foodborne Viruses on Fruits and Vegetables

Application of an Optimized Direct Lysis Method for Viral RNA Extraction Linking Contaminated Dates to Infection With Hepatitis A Virus

“…To address the analytical challenge, we recently reported a further step toward improvement of the direct lysis method to allow for higher recovery of the process controls (mengovirus and murine norovirus, MNV) and less inhibited RT-PCR detection of the target viruses (NoV and HAV) in extracts from 13 different types of foods; fruits (including dates), vegetables, a compound food (seaweed salad) and seeds/nuts. The performance of the optimized direct lysis method was compared against a modified version of the ISO 15216-1 standard, and demonstrated successful detection of NoV in naturally contaminated strawberries and seaweed salad (Rajiuddin et al, 2020). However, the method's broadness in detecting natural contamination remains to be shown for other viral pathogens and food matrices (Rajiuddin et al, 2020).…”

“…The performance of the optimized direct lysis method was compared against a modified version of the ISO 15216-1 standard, and demonstrated successful detection of NoV in naturally contaminated strawberries and seaweed salad (Rajiuddin et al, 2020). However, the method's broadness in detecting natural contamination remains to be shown for other viral pathogens and food matrices (Rajiuddin et al, 2020).…”

Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

“…For virus detection in food, there are mainly two types of techniques, culture/counting methods and polymerase chain reaction (PCR) based methods [7,8]. Because the culture process is very time-consuming, PCR based methods have become a mainstream approach [8,15]. Numerous works on various virus detection by PCR or reverse transcription (RT)-PCR in food have been reported, including hepatitis A [15][16][17], hepatitis E [18], norovirus [15][16][17].…”

“…Because the culture process is very time-consuming, PCR based methods have become a mainstream approach [8,15]. Numerous works on various virus detection by PCR or reverse transcription (RT)-PCR in food have been reported, including hepatitis A [15][16][17], hepatitis E [18], norovirus [15][16][17]. Although PCR techniques have been developed for more than 30 years, the operation is still complicated, and the required turnaround time cannot be shorter than several hours [15].…”

“…Numerous works on various virus detection by PCR or reverse transcription (RT)-PCR in food have been reported, including hepatitis A [15][16][17], hepatitis E [18], norovirus [15][16][17]. Although PCR techniques have been developed for more than 30 years, the operation is still complicated, and the required turnaround time cannot be shorter than several hours [15]. Based on the good specificity and versatile sensing mechanisms, bio-probe based sensors for virus detection are becoming more practicable in recent years as promising approaches [19][20][21].…”