An optimised mouse model of chronic pancreatitis with a combination of ethanol and cerulein

Ahmadi, Abbas; Nikkhoo, Bahram; Mokarizadeh, Aram; Rahmani, Mohammadreza; Fakhari, Shohreh; Mohammadi, Mehdi; Jalili, Ali

doi:10.5114/ceji.2016.58816

Cited by 13 publications

(7 citation statements)

References 42 publications

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“…However, alcohol ingestion alone did not induce chronic pancreatitis, despite long experimental durations, and a combination of alcohol and various agents such as cerulein is needed. 163 Recurrent episodes of acute pancreatitis frequently lead to chronic pancreatic injury in humans. The cerulein-induced chronic pancreatitis model consists in repeated bouts of cerulein-induced acute pancreatitis in the course of several weeks causing chronic injury to the pancreas.…”

Section: Carcinogen-induced Pdac In Micementioning

confidence: 99%

Current status of targeted microbubbles in diagnostic molecular imaging of pancreatic cancer

Jugniot

Bam

Meuillet

et al. 2020

Bioengineering & Transla Med

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Pancreatic ductal adenocarcinoma (PDAC) is often associated with a poor prognosis due to silent onset, resistance to therapies, and rapid spreading. Most patients are ineligible for curable surgery as they present with advanced disease at the time of diagnosis. Present diagnostic methods relying on anatomical changes have various limitations including difficulty to discriminate between benign and malignant conditions, invasiveness, the ambiguity of imaging results, or the inability to detect molecular biomarkers of PDAC initiation and progression. Therefore, new imaging technologies with high sensitivity and specificity are critically needed for accurately detecting PDAC and noninvasively characterizing molecular features driving its pathogenesis. Contrast enhanced targeted ultrasound (CETUS) is an upcoming molecular imaging modality that specifically addresses these issues. Unlike anatomical imaging modalities such as CT and MRI, molecular imaging using CETUS is promising for early and accurate detection of PDAC. The use of molecularly targeted microbubbles that bind to neovascular targets can enhance the ultrasound signal specifically from malignant PDAC tissues. This review discusses the current state of diagnostic imaging modalities for pancreatic cancer and places a special focus on ultrasound targetedmicrobubble technology together with its clinical translatability for PDAC detection.

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Section: Carcinogen-induced Pdac In Micementioning

confidence: 99%

Current status of targeted microbubbles in diagnostic molecular imaging of pancreatic cancer

Jugniot

Bam

Meuillet

et al. 2020

Bioengineering & Transla Med

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“…The rats exhibited progressive pancreatic injury with acinar atrophy, infiltration mainly by neutrophils and macrophages, edema, stellate cell activation, and fibrosis (49 (53), which involves acinar atrophy, parenchymal fibrosis, and inflammatory infiltrates. That model featured a strong clinical relevance and, in contrast to feeding alcohol, intraperitoneal injections of ethanol had greater reproducibility (52). These observations show that ethanol intake is a disease modifier, leading to changes in the normal inflammatory response, supporting the observance that alcohol is associated with the rapid progression of CP in humans (20).…”

Section: Alcohol-induced Murine Cpmentioning

confidence: 53%

Genetically inducedvs.classical animal models of chronic pancreatitis: a critical comparison

et al. 2018

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Chronic pancreatitis (CP) is an utmost complex disease that is pathogenetically linked to pancreas-intrinsic ( e.g., duct obstruction), environmental-toxic ( e.g., alcohol, smoking), and genetic factors. Studying such a complex disease naturally requires validated experimental models. In the past 2 decades, the various animal models of CP usually addressed either the pancreas-intrinsic ( e.g., the caerulein model), the environmental-toxic ( e.g., diet-induced models), or the genetic component of CP. As such, these models were far from mirroring CP in its full spectrum, and the correct choice of models was vital for valid scientific conclusions on CP. The quest for mechanistic, genetic models gave rise to models based on gene modification and transgene insertion, such as the PRSS1 and the IL-1β/IL-1β models. Recently, we witnessed the development of highly exciting models that rely on the importance of autophagy in CP, that is, the murine pancreas-specific Atg5 and LAMP2 knockout models. Today, critical comparison of these several models is more important than ever for guiding research on CP in an efficient direction. The present review outlines the characteristics of the new genetic models in comparison with the well-known classic models for CP, notes the caveats in the choice of models, and also indicates novel directions for model development.-Klauss, S., Schorn, S., Teller, S., Steenfadt, H., Friess, H., Ceyhan, G. O., Demir, I. K. Genetically induced vs. classical animal models of chronic pancreatitis: a critical comparison.

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“…Second, in animal models of CP, recurrent injury to the pancreas with cerulein-induced hyperstimulation to causing AP and RAP leads to pancreatic fibrosis (39–42). Discontinuing cerulein injections leads to resolution of the fibrosis and regeneration of normal pancreatic tissue, depending on the severity of damage over time (39).…”

Section: Resultsmentioning

confidence: 99%

International consensus statements on early chronic Pancreatitis. Recommendations from the working group for the international consensus guidelines for chronic pancreatitis in collaboration with The International Association of Pancreatology, American Pancreatic Association, Japan Pancreas Society, PancreasFest Working Group and European Pancreatic Club

et al. 2018

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An optimised mouse model of chronic pancreatitis with a combination of ethanol and cerulein

Cited by 13 publications

References 42 publications

Current status of targeted microbubbles in diagnostic molecular imaging of pancreatic cancer

Current status of targeted microbubbles in diagnostic molecular imaging of pancreatic cancer

Genetically inducedvs.classical animal models of chronic pancreatitis: a critical comparison

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