An optimized aptamer-binding viral tegument protein VP8 inhibits the production of Bovine Herpesvirus-1 through blocking nucleocytoplasmic shuttling

Aptamer-based diagnostic and therapeutic approaches in animals: Current potential and challenges

“…However, clinical trials have not yet been done to use them as therapeutic molecules. Similarly, Xu et al, 2019 , Xu et al, 2017 demonstrated that specific DNA aptamers can inhibit the production of Bovine herpesvirus 1 through blocking nucleocytoplasmic shuttling and inhibiting the infectivity of the virus by blocking viral entry. The developed aptamers exhibited high affinity and specificity for Bovine herpesvirus 1 with a Kd value of 3.519 nM and significantly reduced viral replication.…”

“…The use of aptamers for the diagnosis of the virus has been extensively studied. Several aptamers have been reported to be effective in several viruses such as Muscovy duck parvovirus ( Lu et al, 2018 ), H5Hx influenza viruses ( Nguyen et al, 2016 ), H5N1 avian influenza virus ( Wu et al, 2008 ), Triple reassortant influenza A virus ( Wongphatcharachai et al, 2013 ), Bovine herpesvirus 1 ( Xu et al, 2019 ), pseudorabies virus ( Shen et al, 2018 ) etc.…”

“… Becskei et al (2008) 10 RNA Spiegelmer Used to block the activity of anorectic and dipsogenic pancreatic hormone amylin. Bilik et al (2007) DNA aptamers 11 DNA aptamer Detection of avian influenza viruses Lee et al (2020) 12 DNA aptamer Detection of H5N1 avian influenza virus Wu et al (2008) 13 DNA aptamer Detection of triple reassortant influenza A virus Wongphatcharachai et al (2013) 14 DNA aptamer Diagnosis of Bovine herpesvirus 1 Xu et al (2019) 15 DNA aptamer Detection of FMD virus Vidic et al (2017) 16 DNA aptamer Competitive fluorescence resonance energy transfer (FRET)-aptamer-based strategy for foot-and-mouth disease (FMD) Bruno et al (2008) 17 DNA aptamer Detection of E. coli strains K88 Li et al (2011) 18 DNA aptamer Detection of Salmonella enterica serovar. Joshi et al (2009) 19 DNA aptamer Nanoparticle-based two-stage aptasensing platform for Campylobacter jejuni and Campylobacter coli.…”

“… Bai et al (2019) 27 DNA aptamer Competitive voltammetric aptasensor for azlocillin β-lactam antibiotic. Chinnappan et al (2020a) 28 DNA aptamer Detection of Bovine herpesvirus 1 Xu et al, 2019 , Xu et al, 2017 ssDNA aptamers 29 ssDNA aptamer Detection of SARS coronavirus nucleocapsid protein Cho et al (2011) 30 ssDNA aptamer HN protein of Bovine Parainfluenza Virus type 3 Cheng et al (2018) 31 ssDNA aptamer Detection of avian influenza H5Nx whole viruses Nguyen et al (2016) 32 ssDNA aptamer Detection of Muscovy duck parvovirus Lu et al (2018) 33 ssDNA aptamer Detection of Staphylococcus aureus Baumstummler et al (2014) 34 ssDNA aptamer An enzyme linked aptamer for the detection of P48 protein of M. bovis Fu et al (2014) 35 ssDNA aptamer Detection of TB from sputum samples Rotherham et al (2012) 36 ssDNA aptamer Analytical assay for detection of brevetoxin. Tian et al (2016) 37 ssDNA aptamer Combined the aptamer with electrochemical SPR to develop an aptasensing platform for detection of ampicillin in real time.…”