An optimized biotechnological system for the production of centellosides based on elicitation and bioconversion of Centella asiatica cell cultures

Abstract

Centella asiatica is a herbaceous plant of Asian traditional medicine. Besides wound healing, this plant is recommended for the treatment or care of various skin conditions such as dry skin, leprosy, varicose ulcers, eczema, and/or psoriasis. Triterpene saponins, known as centellosides, are the main metabolites associated with these beneficial effects. Considering the interest in these high value active compounds, there is a need to develop biosustainable and economically viable processes to produce them. Previous work using C. asiatica plant cell culture technology demonstrated the efficient conversion of amyrin derivatives into centellosides, opening a new way to access these biomolecules. The current study was aimed at increasing the production of centellosides in C. asiatica plant cell cultures. Herein, we report the application of a new elicitor, coronatine, combined with the addition of amyrin‐enriched resins as potential sustainable precursors in the centelloside pathway, for a positive synergistic effect on centelloside production. Our results show that coronatine is a powerful elicitor for increasing centelloside production and that treatments with sustainable natural sources of amyrins enhance centelloside yields. This process can be scaled up to an orbitally shaken CellBag, thereby increasing the capacity of the system for producing biomass and centellosides.

Elicitation with methyl jasmonate combined with cultivation in the Plantform™ temporary immersion bioreactor highly increases the accumulation of selected centellosides and phenolics in Centella asiatica (L.) Urban shoot culture

“…Our results with Plantform™ system, in comparison with earlier research of other authors on different types of C. asiatica cultures (e.g ), seem to be very interesting and promising (Table ).…”

“…There is a considerable research on the production of centellosides in suspension and hairy root cultures of C. asiatica but yields of the compounds have been generally low. The highest production was achieved in cultures treated with elicitors . Only few studies concern centellosides production in shoot (or whole plant) cultures and none of them were conducted in temporary immersion bioreactors RITA ® or Plantform™ .…”

In Vitro Study of the Anticancer Effects of Biotechnological Extracts of the Endangered Plant Species Satureja Khuzistanica

“…Taken as a whole, these results demonstrate the suitability of the orbitally shaken CellBag for scaling up the suspension cultures of S. khuzistanica . Similar results have been achieved with Centella asiatica cell cultures, where growth and centelloside production also improved at bioreactor level [18]. Comparison of biomass and RA production of the S. khuzistanica cell line obtained in the wave-mixed and the orbitally shaken bag [7] revealed that both bioreactor systems are effective.…”

Effects of coronatine elicitation on growth and metabolic profiles of Lemna paucicostata culture

“…paucicostata with three coronatine treatment timings (day 0, 23, and 28) compared to the control. In previous studies, it was reported that 1 μM coronatine inhibited the growth of cultured Vitis vinifera and Centella asiatica cells and cotton seedlings [ 29 , 30 , 31 ], whereas the growth of Taxus media cell cultures treated with 1 μM coronatine showed similar pattern to that of un-treated culture [ 19 ]. The effects of coronatine treatment on the dry weights of one frond of L .…”