An Organophosphate Sensor Based on Photo-crosslinked Hydrogel-entrapped E. coli

Fleischauer, Valerie E.; Heo, Jeong Wook

doi:10.2116/analsci.30.937

Cited by 6 publications

(4 citation statements)

References 31 publications

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“…This process did not compromise the sensitivity of the sensor to heavy metals in real water. [200] Additionally, E. coli, embedded in PEG diacrylate (with better mechanical strength than SA) [201] or agar (without chemical crosslinking) [202,203] enabled the detection of environmental toxicity in water. Agar-embedded E. coli-based BLHs were used for continuous monitoring and early warning of water toxicity.…”

Section: Living Luminescent Microorganisms Immobilized In Hydrogelsmentioning

confidence: 99%

Application of Optical Hydrogels in Environmental Sensing

Yang

Sarkar

Xie

et al. 2023

Energy & Environ Materials

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The ever‐increasing complexity of environmental pollutants urgently warrants the development of new detection technologies. Sensors based on the optical properties of hydrogels enabling fast and easy in situ detection are attracting increasing attention. In this paper, the data from 138 papers about different optical hydrogels (OHs) are extracted for statistical analysis. The detection performance and potential of various types of OHs in different environmental pollutant detection scenarios were evaluated and compared to those obtained using the standard detection method. Based on this analysis, the target recognition and sensing mechanisms of two main types of OHs are reviewed and discussed: photonic crystal hydrogels (PCHs) and fluorescent hydrogels (FHs). For PCHs, the environmental stimulus response, target receptors, inverse opal structures, and molecular imprinting techniques related to PCHs are reviewed and summarized. Furthermore, the different types of fluorophores (i.e., compound probes, biomacromolecules, quantum dots, and luminescent microbes) of FHs are discussed. Finally, the potential academic research directions to address the challenges of applying and developing OHs in environmental sensing are proposed, including the fusion of various OHs, introduction of the latest technologies in various fields to the construction of OHs, and development of multifunctional sensor arrays.

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Section: Living Luminescent Microorganisms Immobilized In Hydrogelsmentioning

confidence: 99%

Application of Optical Hydrogels in Environmental Sensing

Yang

Sarkar

Xie

et al. 2023

Energy & Environ Materials

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show abstract

“…However, this process did not compromise the sensitivity of the sensor to heavy metals in real water [18] . Additionally, E. coli , embedded in PEG diacrylate (with better mechanical strength than SA) [209] or agar (without chemical crosslinking) [210,211] enabled the detection of environmental toxicity in water. Agarembedded E. coli -based BLHs were used for continuous monitoring and early warning of water toxicity.…”

Section: Living Luminescent Microorganisms Immobilized In Hydrogelsmentioning

confidence: 99%

Application of optical hydrogels in environmental sensing

Yang

Sarkar

Xie

et al. 2023

Preprint

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The ever-increasing complexity of environmental pollutants urgently warrants the development of new detection technologies. In this context, sensors based on the optical properties of hydrogels enabling fast and easy in situ detection are attracting increasing attention. Herein, the target recognition and sensing mechanisms of two main types of optical hydrogels (OHs) are reviewed and discussed: photonic crystal hydrogels (PCHs) and fluorescent hydrogels (FHs). For PCHs, the environmental stimulus response, target receptors, inverse opal structures, and molecular imprinting techniques related to PCHs are reviewed and summarized. Furthermore, the different types of fluorophores (i.e., compound probes, biomacromolecules, quantum dots, and luminescent microbes) of FHs are summarized. Finally, the data from 138 papers about different OHs are extracted for secondary statistical analysis. The detection performance and potential of various OH types in different environmental pollutant detection scenarios are evaluated, and compared them to those obtained using the standard detection method. Based on this analysis, some possible development directions are proposed, including the fusion of various OHs, introduction of more hydrogel technologies from the biomedical field to the environmental pollutant detection field, and development of multifunctional sensor arrays.

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“…Для усиления первичного аналитического сигнала в рекомбинантных клетках микроорганизмов часто дополнительно повышают уровень биосинтеза фермента, необходимого для детекции ФОС [99][100][101][102][103][104][105][106]. В то же время методы генетической инженерии позволяют точно управлять локализацией фермента в клетке в клеточной стенке, периплазме, цитоплазме после экспрессии соответствующего гена.…”

Section: химическая безопасность и защита от химического терроризмаunclassified

“…Вместе с облегченной диффузией ФОС извне также усиливается и поток клеточного содержимого наружу вплоть до потери клетками их целостности при любых механических воздействиях (например, при перемешивании, промывке буферными растворами и др.). Чтобы избежать такого нарушения целостности, частично пермеабилизованные клетки обрабатывают различными сшивающими агентами, покрывают гелевыми матрицами, включают в полимерные носители и т.д., то есть применяют различные методы иммобилизации и стабилизации клеток [99,103]. Для регистрации аналитического сигнала от ферментов, находящихся внутри подобных клеток, применяют те же самые оптические, электрохимические и прочие указанные выше методы, позволяющие осуществлять детекцию [108].…”

Section: химическая безопасность и защита от химического терроризмаunclassified

Enzymes and Their Forms Used in Detection of Organophosphorus Compounds

2021

Journal of NBC Protection Corps

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Еnzymes are able to effectively interact with various organophosphorus compounds (OPC), entering into (bio)chemical reactions with them. Changes in the initial activity of enzymes as a result of their inhibition by OPC, the formation of OPC degradation products under the action of hydrolytic enzymes, etc. can be determined using different physical and chemical methods and used in bioanalytic systems to determine the concentrations of OPC. The purpose of the review is to analyze the main scientific results achieved over the past 10 years in the development of analytical systems based on enzymes intended for the determination of OPC. It is shown in the article, that the requirements for the sensitivity of biosensors are based on the norms of the content of the analyzed substances detected in/at the objects of mandatory control. The cholinesterases compose a basis for the development of the largest number of ultra-sensitive biosensors, although other enzymes can be successfully used as a biosensitive element. The most technologically advanced solution that is close to the practical implementation seems to be bioanalytical systems using immobilized enzymes. Improving the detection limits of the OPC can be achieved by using nanoobjects together with modern methods of signal transducers, for example, with nanomechanical detectors and signal converters. This combination of technical solutions ensures the sensitivity of the OPC analysis up to pg/l. At present, «reagentless» systems have received significant development, which have become the basis for the production of a large number of commercially available strips for the express determination of OPC. Modern demands stimulate the rapid development of portable and, especially, wearable biosensors that can be attached to various surfaces, including a clothing. The progress in the development of affine amino acid sequences, in the future, will allow the creation of enzyme biosensors on any surface.

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An Organophosphate Sensor Based on Photo-crosslinked Hydrogel-entrapped E. coli

Cited by 6 publications

References 31 publications

Application of Optical Hydrogels in Environmental Sensing

Application of Optical Hydrogels in Environmental Sensing

Application of optical hydrogels in environmental sensing

Enzymes and Their Forms Used in Detection of Organophosphorus Compounds

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