An Overview of Collagen-Based Composite Scaffold for Bone Tissue Engineering

Vijayalekha, Ashwathi; Anandasadagopan, Suresh Kumar; Pandurangan, Ashok Kumar

doi:10.1007/s12010-023-04318-y

Cited by 13 publications

(2 citation statements)

References 74 publications

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“…Collagen is one of the most widely used hydrogel scaffolds for miRNA delivery in bone tissue engineering, as it is the major organic component of the bone extracellular matrix [137]. Collagen exhibits excellent biocompatibility, the ability to combine with other materials, high porosity, low antigenicity, ease of processing, hydrophilicity, and excellent absorbability [138]. Collagen also an optimal supporting structure for cell attachment and development [139].…”

Section: Mirna-activated Scaffold and Bone Regenerationmentioning

confidence: 99%

Mimic miRNA and Anti-miRNA Activated Scaffolds as a Therapeutic Strategy to Promote Bone, Cartilage, and Skin Regeneration

Guelfi,

Capaccia,

Anipchenko

et al. 2024

Macromol

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MiRNA-based therapies represent an innovative and promising strategy applicable to various medical fields, such as tissue regeneration and the treatment of numerous diseases, including cancer, cardiovascular problems, and viral infections. MiRNAs, a group of small non-coding RNAs, play a critical role in regulating gene expression at the post-transcriptional level and modulate several signaling pathways that maintain cellular and tissue homeostasis. The clinical trials discussed in the review herald a new therapeutic era for miRNAs, particularly in tissue engineering, using synthetic exogenous mimic miRNAs and antisense miRNAs (anti-miRNAs) to restore tissue health. This review provides an overview of miRNAs’ biogenesis, mechanism of action, regulation, and potential applications, followed by an examination of the challenges associated with the transport and delivery of therapeutic miRNAs. The possibility of using viral and non-viral vectors that protect against degradation and ensure effective miRNA delivery is highlighted, focusing on the advantages of the emerging use of 3D biomaterial scaffolds for the delivery of mimic miRNAs and anti-miRNAs to facilitate tissue repair and regeneration. Finally, the review assesses the current landscape of miRNA-activated scaffold therapies on preclinical and clinical studies in bone, cartilage, and skin tissues, emphasizing their emergence as a promising frontier in personalized medicine.

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Section: Mirna-activated Scaffold and Bone Regenerationmentioning

confidence: 99%

Mimic miRNA and Anti-miRNA Activated Scaffolds as a Therapeutic Strategy to Promote Bone, Cartilage, and Skin Regeneration

Guelfi,

Capaccia,

Anipchenko

et al. 2024

Macromol

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“…This mechanical support is crucial for providing a stable scaffold for bone formation and preventing bone collapse. The dual-layer structure of the DLC, made out of collagen and glycosaminoglycans, facilitates cell infiltration allowing osteoprogenitor cells to migrate into the scaffold and differentiate into osteoblasts, the bone-forming cells [11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Glycosaminoglycans Modulate the Angiogenic Ability of Type I Collagen-Based Scaffolds by Acting on Vascular Network Remodeling and Maturation

Salvante,

Popoiu,

Saxena

et al. 2024

Bioengineering

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Type I collagen, prevalent in the extracellular matrix, is biocompatible and crucial for tissue engineering and wound healing, including angiogenesis and vascular maturation/stabilization as required processes of newly formed tissue constructs or regeneration. Sometimes, improper vascularization causes unexpected outcomes. Vascularization failure may be caused by extracellular matrix collagen and non-collagen components heterogeneously. This study compares the angiogenic potential of collagen type I-based scaffolds and collagen type I/glycosaminoglycans scaffolds by using the chick embryo chorioallantoic membrane (CAM) model and IKOSA digital image analysis. Two clinically used biomaterials, Xenoderm (containing type I collagen derived from decellularized porcine extracellular matrix) and a dual-layer collagen sponge (DLC, with a biphasic composition of type I collagen combined with glycosaminoglycans) were tested for their ability to induce new vascular network formation. The AI-based IKOSA app enhanced the research by calculating from stereomicroscopic images angiogenic parameters such as total vascular area, branching sites, vessel length, and vascular thickness. The study confirmed that Xenoderm caused a fast angiogenic response and substantial vascular growth, but was unable to mature the vascular network. DLC scaffold, in turn, produced a slower angiogenic response, but a more steady and organic vascular maturation and stabilization. This research can improve collagen-based knowledge by better assessing angiogenesis processes. DLC may be preferable to Xenoderm or other materials for functional neovascularization, according to the findings.

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