An Overview of Microalgae Lipid Extraction in a Biorefinery Framework

Roux, Jean-Maxime; Lamotte, Hadrien; Achard, Jean‐Luc

doi:10.1016/j.egypro.2017.03.1137

Cited by 94 publications

(39 citation statements)

References 44 publications

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“…A wide array of products, ranging from proteins to lipids and various other compounds can be sourced from them [1]. Microalgal lipids, more specifically, were initially considered as an excellent substitute for biodiesel with the focus of microalgae utilization nowadays slowly shifting to other applications such as aqua-feed and cosmetics industries [2]. A considerable bottleneck to any large scale commercial exploitation of microalgae, however, is the high cost of the extraction of intracellular components which in the case of biodiesel can represent 30 to 50% of the overall process [3].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of pulsed electric fields effect on the microalgae cell mechanical stability through high pressure homogenization

et al. 2020

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Pulsed Electric Fields (PEF) is a known technique for the permeabilization of cell membranes, which can considerably foster intracellular component extraction from microalgae. During this phenomenon, the cells are subjected to short electrical pulses leading to the deconstruction of the cell membrane. However, it is currently uncertain in what way, if any, the microalgae cell wall is affected during pulsing. In this study, freshly harvested Auxenochlorella protothecoides (AP) and Chlorella vulgaris (CV) were subjected to PEF treatment with an energy input of 1.5MJ per kilogram of dry matter and then fed into a High Pressure Homogenizer (HPH) for 5 passes at 1500 bar. The percentage of intact cells after each pass was determined and compared with Control biomass that underwent the same homogenization. AP and CV autotrophic had almost 40% intact cells at the end of homogenizing whereas AP mixotrophic 20%. In all cases, no major difference on the disruption degree of pulsed and control samples was observed, indicating that the resistance to mechanical stress of the cell, a function of the cell wall, is not affected by PEF. Scanning Electron Microscopy (SEM) observation of the cells also showed no superficial or structural cell alteration after pulsation.

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Section: Introductionmentioning

confidence: 99%

Evaluation of pulsed electric fields effect on the microalgae cell mechanical stability through high pressure homogenization

et al. 2020

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“…In this study, lipid was directly extracted from wet biomass instead of dry biomass to determine the yield. Dewatering technology is an expensive, time-and energy-consuming downstream process in lipid extraction of the industrial scale (Halim et al, 2012;Sathish and Sims, 2012;Roux et al, 2017). When lipid is extracted directly from wet biomass, the overall cost is reduced and the process is more energy efficient (Yang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…However, efficient lipid extraction is often achieved with the combination of solvent extraction and pretreatment steps that disrupt the cell structure (Forfang et al, 2017). Acid hydrolysis prior to extraction weakens the yeast cell wall, causing monomeric sugars to be released into the aqueous phase, enabling the solvents to bind the lipids directly (Yu et al, 2014;Dong et al, 2016;Roux et al, 2017). Up to 97% of total fatty acids was recovered through extraction with hexane after acid pre-treatment (Laurens et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Up to 97% of total fatty acids was recovered through extraction with hexane after acid pre-treatment (Laurens et al, 2015). Generally, both acid hydrolysis and homogenization function to disrupt the cells for better lipid accessibility and extraction efficiency (Halim et al, 2012;Dong et al, 2016;Roux et al, 2017). The rupture of cells through pre-treatment steps reduces the amount of solvents used during extraction compared to untreated biomass (Mercer and Armenta, 2011;Naghdi et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Development of Rhodotorula mucilaginosa strain via random mutagenesis for improved lipid production

Wong¹,

Vincent²

2019

MJM

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Aims:Oleaginous yeasts are widely used for the production of biodiesel feedstocks because of their high lipid content. This research was aimed to conduct random mutagenesis of Rhodotorula mucilaginosa using ethyl methane sulfonate (EMS) and identify the mutants with improved lipid production. Methodology and results: A total of twenty-two mutant isolates prescreened with cerulenin were produced and further characterized via M13 PCR fingerprinting to determine their polymorphism and genetic distances. Eight strains, namely M1, M2, M3, M4, M7, M10, M11 and M18, were chosen based on their genetic distances from the parental strain for biomass production. Six mutants (M1, M2, M3, M4, M7 and M18) showing the highest dry cell weights were further selected for evaluation of lipid production in a laboratory-scale bioreactor using glucose as a carbon source. Results indicated that parental strain exhibited lipid content of 1.83 g/L, while strains M1, M2, M3, M7 and M18 generated 2.37 g/L, 2.27 g/L, 2.27 g/L, 3.10 g/L and 3.83 g/L of intracellular lipid, respectively. These five mutants were identified to have significant increase in lipid production compared to the parental strain. Conclusion, significance and impact of study: This study demonstrated enhanced lipid production in R. mucilaginosa by random mutagenesis. New generated strains had higher lipid productivity compared to parental strain and application of these strains in industry may reduce the overall cost of biodiesel production.

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“…Biofuels have gained great attention as the most sustainable and environmental friendly energy source given their advantages of reproducibility, resource abundance, and lower environmental impacts [1][2][3]. Microalgae is an attractive feedstock for biofuel production due to its high photosynthetic efficiency to convert solar energy and carbon dioxide into oxygen and biomass and its growth on a nonarable land [4]. These unicellular organisms synthesize lipids, carbohydrate and proteins [5].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of in-situ transient simultaneous cell disruption and transesterification of microalgae

González-Delgado¹,

Barajas-Solano²,

Peralta-Ruiz³

2017

ces

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Microalgae has recently been highlighted as source of valuable products including biofuel. The production process of biofuels from microalgae involves mass cultivation, harvesting, deep dewatering, lipid extraction and biofuel conversion. In this work, lipids from microalgae Navicula sp. were obtained using multifunctional process that consists of acid hydrolysis or cellular disruption, oil extraction and in situ transesterification. The effect of alcohol added to produce ethyl and methyl esters on lipid extraction efficiency was evaluated using methanol and ethanol in order to determine the most suitable route for obtaining the high values of lipids 1320 Ángel Darío González-Delgado et al.and total reducing sugar. The highest lipid extraction efficiency and total reducing sugar (7.72 % and 2.63 mg/ml, respectively) was obtained for methanol. The low lipid extraction efficiency of multifuctional process is due to transesterification of lipids that gradually released into the system. The formation of alkyl esters was confirmed by FTIR with an increase in carbonyl peak as the reaction progressed, thus muntifuctional process reduce cost of alkyl esters production by eliminating the step of lipid extraction by solvent.

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An Overview of Microalgae Lipid Extraction in a Biorefinery Framework

Cited by 94 publications

References 44 publications

Evaluation of pulsed electric fields effect on the microalgae cell mechanical stability through high pressure homogenization

Evaluation of pulsed electric fields effect on the microalgae cell mechanical stability through high pressure homogenization

Development of Rhodotorula mucilaginosa strain via random mutagenesis for improved lipid production

Evaluation of in-situ transient simultaneous cell disruption and transesterification of microalgae

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