An overview on application of phage display technique in immunological studies

Rami, Abbas; Behdani, Mahdi; Yardehnavi, Najmeh; Habibi‐Anbouhi, Mahdi; Kazemi‐Lomedasht, Fatemeh

doi:10.1016/j.apjtb.2017.06.001

Cited by 38 publications

(33 citation statements)

References 45 publications

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“…Phage display is a potent technology that consists on the expression of either peptides or proteins fused to coat proteins of the phage’s surface [ 11 , 19 , 20 ]. This technique has been used in biotechnology for several applications such as: directed evolution [ 21 ], identification of ligand binding sites (protein-protein, protein-ligand and protein-DNA interactions) [ 20 ], selection of monoclonal antibodies [ 22 ], protein-based drug discovery [ 23 ], development of epitope-based diagnostic tools, identification of B-cell epitopes [ 24 ] and for vaccine development [ 19 , 25 ]. Filamentous phages (M13, fd, and f1), lytic phages (T4 and T7) and the temperate phage lambda (λ) have been successfully used as display systems [ 10 , 19 , 20 , 26 ].…”

Section: Phage-based Vaccinesmentioning

confidence: 99%

Bacteriophage-Based Vaccines: A Potent Approach for Antigen Delivery

et al. 2020

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Vaccines are considered one of the most important bioproducts in medicine. Since the development of the smallpox vaccine in 1796, several types of vaccines for many diseases have been created. However, some vaccines have shown limitations as high cost and low immune responses. In that regard, bacteriophages have been proposed as an attractive alternative for the development of more cost-effective vaccines. Phage-displayed vaccines consists in the expression of antigens on the phage surface. This approach takes advantage of inherent properties of these particles such as their adjuvant capacity, economic production and high stability, among others. To date, three types of phage-based vaccines have been developed: phage-displayed, phage DNA and hybrid phage-DNA vaccines. Typically, phage display technology has been used for the identification of new and protective epitopes, mimotopes and antigens. In this context, phage particles represent a versatile, effective and promising alternative for the development of more effective vaccine delivery systems which should be highly exploited in the future. This review describes current advances in the development of bacteriophage-based vaccines, with special attention to vaccine delivery strategies. Moreover, the immunological aspects of phage-based vaccines, as well as the applications of phage display for vaccine development, are explored. Finally, important challenges and the future of phage-bases vaccines are discussed.

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Section: Phage-based Vaccinesmentioning

confidence: 99%

Bacteriophage-Based Vaccines: A Potent Approach for Antigen Delivery

et al. 2020

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“…Phage display is a technique of decorating a phage capsid from outside with peptides or proteins, the genetic material of which rests inside the phage capsid or head, discovered in 1985 by Smith and gained recognition through the Nobel prize in chemistry for 2018. Phage display has a role in delivery of therapeutic agents including genes, vaccines and drugs.…”

Section: Why Phage Therapy?mentioning

confidence: 99%

The dawn of phage therapy

Rehman

Ali

Khan

et al. 2019

Reviews in Medical Virology

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Bacteriophages or phages, being the most abundant entities on earth, represent a potential solution to a diverse range of problems. Phages are successful antibacterial agents whose use in therapeutics was hindered by the discovery of antibiotics. Eventually, because of the development and spread of antibiotic resistance among most bacterial species, interest in phage as therapeutic entities has returned, because their noninfectious nature to humans should make them safe for human nanomedicine.This review highlights the most recent advances and progress in phage therapy and bacterial hosts against which phage research is currently being conducted with respect to food, human, and marine pathogens. Bacterial immunity against phages and tactics of phage revenge to defeat bacterial defense systems are also summarized.We have also discussed approved phage-based products (whole phage-based products and phage proteins) and shed light on their influence on the eukaryotic host with respect to host safety and induction of immune response against phage preparations.Moreover, creation of phages with desirable qualities and their uses in cancer treatment, vaccine production, and other therapies are also reviewed to bring together evidence from the scientific literature about the potentials and possible utility of phage and phage encoded proteins in the field of therapeutics and industrial biotechnology.

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“…The helical capsid is composed of approximately 2700 copies of the major pVIII coat protein and is capped with 5 copies each of the pIII, pVI, pVII, and pIX minor coat proteins [77]. The process of phage display, which utilizes the ease of genetic manipulation to modify the surface proteins the M13 phage [78], has enabled this simple phage to be used for multiple purposes including peptide mapping [79], antigen presentation [80,81], as well as a therapeutic carrier and bioconjugation scaffold [82].…”

Section: Virus-based Protein Nanotubes (Pnts)mentioning

confidence: 99%

Protein Nanotubes: From Bionanotech towards Medical Applications

et al. 2019

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Nanobiotechnology involves the study of structures found in nature to construct nanodevices for biological and medical applications with the ultimate goal of commercialization. Within a cell most biochemical processes are driven by proteins and associated macromolecular complexes. Evolution has optimized these protein-based nanosystems within living organisms over millions of years. Among these are flagellin and pilin-based systems from bacteria, viral-based capsids, and eukaryotic microtubules and amyloids. While carbon nanotubes (CNTs), and protein/peptide-CNT composites, remain one of the most researched nanosystems due to their electrical and mechanical properties, there are many concerns regarding CNT toxicity and biodegradability. Therefore, proteins have emerged as useful biotemplates for nanomaterials due to their assembly under physiologically relevant conditions and ease of manipulation via protein engineering. This review aims to highlight some of the current research employing protein nanotubes (PNTs) for the development of molecular imaging biosensors, conducting wires for microelectronics, fuel cells, and drug delivery systems. The translational potential of PNTs is highlighted.

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An overview on application of phage display technique in immunological studies

Cited by 38 publications

References 45 publications

Bacteriophage-Based Vaccines: A Potent Approach for Antigen Delivery

Bacteriophage-Based Vaccines: A Potent Approach for Antigen Delivery

The dawn of phage therapy

Protein Nanotubes: From Bionanotech towards Medical Applications

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