An Oxygen-Insensitive biosensor and a biofuel cell device based on FMN l-lactate dehydrogenase

Cohen, Roy; Herzallh, Nidaa S.; Meirovich, Matan M.; Bachar, Oren; Frech, Liora; Cohen, Yifat; Yehezkeli, Omer

doi:10.1016/j.bioelechem.2022.108316

Cited by 6 publications

(2 citation statements)

References 45 publications

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“…The first reported Fc b 2 -based ABSs were developed using cells or cell lysates, from the yeasts S. cerevisiae and Hansenula anomala as the parental cells of this enzyme [ 30 , 31 ]. Many Lact-sensitive ABSs based on the recombinant cells have been proposed, especially in the last several years [ 32 , 33 , 34 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Flavocytochrome b2-Mediated Electroactive Nanoparticles for Developing Amperometric L-Lactate Biosensors

et al. 2023

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L-Lactate is an indicator of food quality, so its monitoring is essential. Enzymes of L-Lactate metabolism are promising tools for this aim. We describe here some highly sensitive biosensors for L-Lactate determination which were developed using flavocytochrome b2 (Fcb2) as a bio-recognition element, and electroactive nanoparticles (NPs) for enzyme immobilization. The enzyme was isolated from cells of the thermotolerant yeast Ogataea polymorpha. The possibility of direct electron transfer from the reduced form of Fcb2 to graphite electrodes has been confirmed, and the amplification of the electrochemical communication between the immobilized Fcb2 and the electrode surface was demonstrated to be achieved using redox nanomediators, both bound and freely diffusing. The fabricated biosensors exhibited high sensitivity (up to 1436 A·M−1·m−2), fast responses, and low limits of detection. One of the most effective biosensors, which contained co-immobilized Fcb2 and the hexacyanoferrate of gold, having a sensitivity of 253 A·M−1·m−2 without freely diffusing redox mediators, was used for L-Lactate analysis in samples of yogurts. A high correlation was observed between the values of analyte content determined using the biosensor and referenced enzymatic-chemical photometric methods. The developed biosensors based on Fcb2-mediated electroactive nanoparticles can be promising for applications in laboratories of food control.

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Section: Introductionmentioning

confidence: 99%

Flavocytochrome b2-Mediated Electroactive Nanoparticles for Developing Amperometric L-Lactate Biosensors

et al. 2023

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“…The development of EFCs is still in its early stages and is associated with lower efficiencies and power outputs compared to other fuel cells, and there is a need for more efficient and stable enzymatic electrodes [7][8][9][10][11]. However, significant contributions concerning the advantages of using a wide range of bio-based fuels, such as glucose, fructose, lactate, ethanol, and hydrogen, instead of fossil fuels have been convincingly demonstrated [12][13][14][15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Sustainably Sourced Mesoporous Carbon Molecular Sieves as Immobilization Matrices for Enzymatic Biofuel Cell Applications

Torrigino,

Nagel,

Peng

et al. 2023

Catalysts

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Ordered mesoporous carbon CMK-3 sieves with a hexagonal structure and uniform pore size have recently emerged as promising materials for applications as adsorbents and electrodes. In this study, using sucrose as the sustainable carbon source and SBA-15 as a template, CMK-3 sieves are synthesized to form bioelectrocatalytic immobilization matrices for enzymatic biofuel cell (EFC) electrodes. Their electrochemical performance, capacitive features, and the stability of enzyme immobilization are analyzed and compared to commercially available multi-walled carbon nanotubes (MWCNT) using cyclic voltammetry and electrochemical impedance spectroscopy (EIS). The anodic reaction in the presence of glucose oxidase (GOx) and ferrocene methanol (FcMeOH) on the sustainably sourced CMK-3-based electrodes produces bioelectrocatalytic current responses at 0.5 V vs. saturated calomel electrode (SCE) that are twice as high as on the MWCNT-based electrodes under saturated glucose conditions. For the cathodic reaction, the MWCNT-based cathode performs marginally better than the CMK-3-based electrodes in the presence of bilirubin oxidase (BOD) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS2−). The CMK-3-based EFCs assembled from the GOx anode and BOD cathode results in a power output of 93 μW cm−2. In contrast, the output power of MWCNT-based EFCs is approximately 53 μW cm−2. The efficiency of CMK-3 as a support material for biofuel cell applications is effectively demonstrated.

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Electrochemical characterization of a dual cytochrome-containing lactate dehydrogenase

Boyarski

Shlush

Paz

et al. 2023

Bioelectrochemistry

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An Oxygen-Insensitive biosensor and a biofuel cell device based on FMN l-lactate dehydrogenase

Cited by 6 publications

References 45 publications

Flavocytochrome b2-Mediated Electroactive Nanoparticles for Developing Amperometric L-Lactate Biosensors

Flavocytochrome b2-Mediated Electroactive Nanoparticles for Developing Amperometric L-Lactate Biosensors

Sustainably Sourced Mesoporous Carbon Molecular Sieves as Immobilization Matrices for Enzymatic Biofuel Cell Applications

Electrochemical characterization of a dual cytochrome-containing lactate dehydrogenase

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