An Oxygen-Insensitive Reagentless Glucose Biosensor Based on Osmium-Complex Modified Polypyrrole

Abstract

An optimized material for the development of reagentless oxygen‐independent biosensors based on conducting polymers is described. Considering the prerequisites for a fast electron transfer between a redox enzyme and the electrode surface via an electron‐hopping mechanism, an Os‐complex‐modified pyrrole derivative with a long, flexible spacer chain has been synthesized. Copolymerization of the new mediator‐modified pyrrole monomer with pyrrole was optimized aiming on a higher mediator loading in the film. The feasibility of this material for the development of reagentless oxygen‐independent biosensors is demonstrated by entrapment of a PQQ‐dependent glucose dehydrogenase isolated from Erwinia sp. 34‐1 within this electrochemically grown redox‐polymer network.

Coupling of an enzymatic biofuel cell to an electrochemical cell for self-powered glucose sensing with optical readout

“…However, the oxidation of other mono-and disaccharides occur with lower catalytic efficiencies. Therefore, this system could be used for the analysis of a single sugar in different samples where glucose is a major constituent including biological fluids [18,19]. For the adequate immobilization of an enzyme to an electrode and in order to obtain a substrate-dependent current flow, the prosthetic group of the enzyme has to be electrochemically recycled at the electrode surface [20].…”

A novel l-lactate-selective biosensor based on flavocytochrome b2 from methylotrophic yeast Hansenula polymorpha

“…Ferrocene was obtained from Merck-Schuchardt (Ottobrunn, Germany), 1,1 -dimethylferrocene from (Aldrich, Deisenhofen, Germany), and methylene blue and glutardialdehyde from Riedel-de Haën (Seelze, Germany). (Os(Me 2 bpy) 2 Cl 2 ) was synthesised according to literature (Habermüller et al, 2000). The used anodic electrodeposition paint "Resydrol AY 498 w" was from Vianova Resins (Mainz-Kastel, Germany), and the cathodic electrodeposition paint "GY 83-0270 0005" was from BASF Farben und Lacke (Münster, Germany).…”

Biosensors based on electropolymerized films: new trends

“…In this context, polypyrrole copolymers containing an osmium complex linked by a long and flexible alkyl chain (Fig. 3), were applied to the immobilization and electrical wiring of glucose dehydrogenase and peroxidase [128,129]. The optimized biosensor configuration allowed oxygen-independent glucose detection.…”