An SSR-based linkage map of Capsicum annuum

Minamiyama, Yasuhiro; Tsuro, Masato; Hirai, Masashi

doi:10.1007/s11032-006-9024-3

Cited by 137 publications

(131 citation statements)

References 38 publications

Supporting

Mentioning

114

Contrasting

Unclassified

Order By: Relevance

“…Based largely on this set, 139 wild types distributed over the entire exploration area were chosen as were 49 domesticated types that are endemic landraces (ancho, puya, and guajillo) (33). This collection was screened with 17 simple sequence repeat (SSR) DNA markers (34,35). These markers were chosen for this study because of their consistency of amplification and polymorphism within our sample.…”

Section: Resultsmentioning

confidence: 99%

Multiple lines of evidence for the origin of domesticated chili pepper,Capsicum annuum, in Mexico

Kraft

Brown

Nabhan

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

252

155

View full text Add to dashboard Cite

The study of crop origins has traditionally involved identifying geographic areas of high morphological diversity, sampling populations of wild progenitor species, and the archaeological retrieval of macroremains. Recent investigations have added identification of plant microremains (phytoliths, pollen, and starch grains), biochemical and molecular genetic approaches, and dating through 14 C accelerator mass spectrometry. We investigate the origin of domesticated chili pepper, Capsicum annuum, by combining two approaches, species distribution modeling and paleobiolinguistics, with microsatellite genetic data and archaeobotanical data. The combination of these four lines of evidence yields consensus models indicating that domestication of C. annuum could have occurred in one or both of two areas of Mexico: northeastern Mexico and central-east Mexico. Genetic evidence shows more support for the more northern location, but jointly all four lines of evidence support central-east Mexico, where preceramic macroremains of chili pepper have been recovered in the Valley of Tehuacán. Located just to the east of this valley is the center of phylogenetic diversity of Proto-Otomanguean, a language spoken in mid-Holocene times and the oldest protolanguage for which a word for chili pepper reconstructs based on historical linguistics. For many crops, especially those that do not have a strong archaeobotanical record or phylogeographic pattern, it is difficult to precisely identify the time and place of their origin. Our results for chili pepper show that expressing all data in similar distance terms allows for combining contrasting lines of evidence and locating the region(s) where cultivation and domestication of a crop began.

show abstract

Section: Resultsmentioning

confidence: 99%

Multiple lines of evidence for the origin of domesticated chili pepper,Capsicum annuum, in Mexico

Kraft

Brown

Nabhan

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

252

155

View full text Add to dashboard Cite

show abstract

“…Several reports describe the development of microsatellite markers for use in genetic studies of Capsicum (Sanwen et al, 2000;Lee et al, 2004;Minamiyama et al, 2006;Yi et al, 2006;Nagy et al, 2007;Portis et al, 2007;Kong et al, 2012;Sugita et al, 2013). However, it can be noted that the number of publicly available molecular markers based on polymerase chain reaction (PCR) for peppers, including SSR markers, is still limited.…”

Section: Introductionmentioning

confidence: 99%

Novel and highly informative Capsicum SSR markers and their cross-species transferability

Buso¹,

Reis²,

Amaral³

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. This study was undertaken primarily to develop new simple sequence repeat (SSR) markers for Capsicum. As part of this project aimed at broadening the use of molecular tools in Capsicum breeding, two genomic libraries enriched for AG/TC repeat sequences were constructed for Capsicum annuum. A total of 475 DNA clones were sequenced from both libraries and 144 SSR markers were tested on cultivated and wild species of Capsicum. Forty-five SSR markers were randomly selected to genotype a panel of 48 accessions of the Capsicum germplasm bank. The number of alleles per locus ranged from 2 to 11, with an average of 6 alleles. The polymorphism information content was on average 0.60, ranging from 0.20 to 0.83. The cross-species transferability to seven cultivated and wild Capsicum species was 2 G.S.C. Buso et al. Genetics and Molecular Research 15 (3): gmr.15038689 tested with a set of 91 SSR markers. We found that a high proportion of the loci produced amplicons in all species tested. C. frutescens had the highest number of transferable markers, whereas the wild species had the lowest. Our results indicate that the new markers can be readily used in genetic analyses of Capsicum.

show abstract

“…In pepper breeding projects, haploid plants and DH lines are often included in studies for gene mapping and the identification of useful recessive mutations (Ochoa-Alejo and Ramirez-Malagon, 2001;Yoo et al, 2003;Minamiyama et al, 2006;Djian-Caporalino et al, 2007;Mimura et al, 2012). The application of PCRbased techniques is becoming important, not only for genetic analysis, but also for variety determination of plants of DH origin (Prince et al, 1995;Parra-Vega et al, 2013b).…”

Section: Perspectives On Anther Culturementioning

confidence: 99%

Pepper (Capsicum annuum L.) anther culture: fundamentalresearch and practical applications

Irikova¹,

Kintzios²,

Grozeva³

et al. 2016

Turk J Biol

View full text Add to dashboard Cite

IntroductionIn general, haploid cells or individuals are those in which the original chromosome number has reduced by half. According to Seguí-Simarro (2010), androgenesis can be defined as a set of biological processes leading to an individual that genetically originated exclusively from a male nucleus. After spontaneous or induced genome doubling, double haploids (DHs) that are completely homozygous are obtained. The production of haploid plants can be induced by methods such as parthenogenesis, interspecific hybridization, pollination with irradiated pollen, hormonal and/or chemical treatment, and application of high-temperature stress (Forster et al., 2007;Murovec and Bohanec, 2013). In anther culture, haploids and DHs occur through direct microspore embryogenesis. This technique has no analogue for fast creation of pure homozygous lines in a single step. In pepper-plant breeding, for example, this method has helped in the development of important new varieties with improved adaptability, productivity, and disease and pest tolerance (Chunling and Baojun, 1995;Venczel and Mitykó, 1995;Arnedo Andrés et al., 2004;Nervo et al., 2007). DH lines are a suitable material for genetic and molecular studies, and this determines their high value in molecular breeding as well (Madhusudhana, 2015).Regarding the genus Capsicum, the first haploids were derived by in vitro anther culture of C. annuum and C.

show abstract

An SSR-based linkage map of Capsicum annuum

Cited by 137 publications

References 38 publications

Multiple lines of evidence for the origin of domesticated chili pepper,Capsicum annuum, in Mexico

Multiple lines of evidence for the origin of domesticated chili pepper,Capsicum annuum, in Mexico

Novel and highly informative Capsicum SSR markers and their cross-species transferability

Pepper (Capsicum annuum L.) anther culture: fundamentalresearch and practical applications

Contact Info

Product

Resources

About