An ultrasensitive chemiluminescence aptasensor for thrombin detection based on iron porphyrin catalyzing luminescence desorbed from chitosan modified magnetic oxide graphene composite

Functional Magnetic Graphene Composites for Biosensing

“…Then immune-magnetic GO composites were synthesized through the interaction of anti-EpCAM antibodies and anti-lgG antibodies. In addition, the chitosan could also be used as the bridge for a non-covalent combination between graphene oxides (GOs) with Fe 3 O 4 nanoparticles [13][14][15]. Others noncovalent bonds, including van der Waals interaction, π-π staking, electrostatic interactions and hydro bonding were also used to building MGCs [10,[16][17][18][19][20].…”

“…Additionally, MGCs realized chemiluminescent or electro-chemiluminescent analysis methods using the properties of the high specific surface area, magnetic separation and more together [8,15,[36][37][38]49,83,84]. As shown in Figure 1d [37], a chemiluminescent aptasensor based on Fe 3 O 4 /GO composites and prostate specific antigen (PSA) aptamers was developed for PSA detection with LOD of 0.5 ng•mL −1 .…”

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

“…The antigen–antibody reaction is the basis of immunoanalysis in clinical laboratories, and modern immuno-labeling techniques using highly sensitive substances as labels have further broadened the scope of immunological techniques. In recent years, oligonucleotide aptamers have exhibited high affinity and specificity, as well as high speed in selection, synthesis, and scale-up, which has attracted great attention in terms of diagnosis and therapeutics [ 37 , 38 , 39 ]. These specific MREs are firstly immobilized on the transducer’s surface as capture probes, and the target proteins can then be specifically recognized by capture probes, linking to the interface of biosensors.…”

“…Generally, to further increase the stability and reaction efficiency of biosensors, biocompatible matrices are needed to immobilize capture probes and to provide the circumstances appropriate for heterogeneous biological reactions [ 37 , 42 ]. Many nanomaterials have been used for the construction of biocompatible matrices, and the matrices could also act as electron transfer media or tracing signal emitters ( Figure 2 ).…”

“…As one of the most sensitive means of detection, CL detection has been widely used for fluidic immunoassay (FIA) [ 70 , 71 , 72 ], forming a combined technique of CL-FIA that has high sensitivity due to the CL and high selectivity due to the FIA. Moreover, CL detection is also a benefit for the construction of ultrasensitive immunosensors [ 3 , 17 , 37 ].…”