An ultrasensitive electrochemical immunosensor for the detection of human immunoglobulin G based on Ag@BSA microspheres

Zhang, Hongfang; Ning, Danlei; Zheng, Jianbin

doi:10.1039/c5ra22654f

Cited by 9 publications

(4 citation statements)

References 33 publications

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“…Most importantly, the high electroactivity of Ag@Au-Fe3O4 nanoelectrocatalyst greatly amplified the signal. Moreover, this Ag@Au-Fe3O4 nanoelectrocatalyst immunosensor can be used repetitively for at least twenty times which may reduce the sensor cost due to its H2O2 reduction based signal resource which is different with our previous work (Ma et al, 2015;Zhang et al, 2015). **the unit of the data is Ω mL ng -1 .…”

Section: Performance Of the Immunosensormentioning

confidence: 90%

A novel electrochemical immunosensor based on nonenzymatic Ag@Au-Fe3O4 nanoelectrocatalyst for protein biomarker detection

Zhang

et al. 2016

Biosensors and Bioelectronics

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A hybrid nanostructure of Fe3O4 nanospheres and Ag@Au nanorods prepared by polydopamine coating was utilized as nanoelectrocatalyst to construct a novel sandwich-type electrochemical immunosensor. Ag@Au-Fe3O4 nanohybrid modified electrode exhibited much better electrocatalytic activity toward the reduction of hydrogen peroxide than Fe3O4 nanospheres or Ag@Au nanorods due to the synergetic catalytic effect. The immunosensor was prepared by immobilizing the capture antibodies on the amine-terminated nanocomposite of carbon nanofibers-chitosan, whilst the trace tag was prepared by loading detection antibodies on the Ag@Au-Fe3O4 nanocomposite. After the parameter optimization, the amperometric signal increased linearly with human IgG concentration in the broad range of 0.1pgmL(-1) to 5μgmL(-1) with a detection limit of 50fgmL(-1). Meanwhile, the enzyme-free catalyst based immunosensor also showed acceptable selectivity, reproducibility and stability.

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Section: Performance Of the Immunosensormentioning

confidence: 90%

A novel electrochemical immunosensor based on nonenzymatic Ag@Au-Fe3O4 nanoelectrocatalyst for protein biomarker detection

Zhang

et al. 2016

Biosensors and Bioelectronics

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“…The current is measured during the stripping step (a square wave) when the analyte is removed from the electrode [49]. Zhang et al chose ASV to detect IgG in human serum [50]. The electrode surface was coated with β-cyclodextrin-functionalized AuNPs (β-CD/Au).…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Biosensors for Detecting Lymphocytes and Immunoglobulins

et al. 2020

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Lymphocytes (B, T and natural killer cells) and immunoglobulins are essential for the adaptive immune response against external pathogens. Flow cytometry and enzyme-linked immunosorbent (ELISA) kits are the gold standards to detect immunoglobulins, B cells and T cells, whereas the impedance measurement is the most used technique for natural killer cells. For point-of-care, fast and low-cost devices, biosensors could be suitable for the reliable, stable and reproducible detection of immunoglobulins and lymphocytes. In the literature, such biosensors are commonly fabricated using antibodies, aptamers, proteins and nanomaterials, whereas electrochemical, optical and piezoelectric techniques are used for detection. This review describes how these measurement techniques and transducers can be used to fabricate biosensors for detecting lymphocytes and the total content of immunoglobulins. The various methods and configurations are reported, along with the advantages and current limitations.

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“…Human immunoglobulin G (HIgG) is the main antibody component in human serum, which plays an important role in defending against various destructive substances, such as viruses or bacteria. [1][2][3][4] The HIgG level in healthy adults ranges from 7 mg mL À1 to 18 mg mL À1 , but this value is usually altered associated with some diseases, such as rheumatoid arthritis, infectious, humoral immune deciency, and liver and metabolic diseases. [5][6][7] Therefore, the detection of HIgG in human serum is important for the early diagnosis and effective treatment of related diseases.…”

Section: Introductionmentioning

confidence: 99%

AuNP array coated substrate for sensitive and homogeneous SERS-immunoassay detection of human immunoglobulin G

Wang

Zeng

et al. 2021

RSC Adv.

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An ultrasensitive electrochemical immunosensor for the detection of human immunoglobulin G based on Ag@BSA microspheres

Abstract: Ag@BSA microspheres were applied as electroactive labels for signal amplification and the ultimate immunosensor exhibited an ultralow detection limit.

Cited by 9 publications

References 33 publications

A novel electrochemical immunosensor based on nonenzymatic Ag@Au-Fe3O4 nanoelectrocatalyst for protein biomarker detection

A novel electrochemical immunosensor based on nonenzymatic Ag@Au-Fe3O4 nanoelectrocatalyst for protein biomarker detection

Biosensors for Detecting Lymphocytes and Immunoglobulins

AuNP array coated substrate for sensitive and homogeneous SERS-immunoassay detection of human immunoglobulin G

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