An ultrasensitive performance enhanced novel cytochrome c biosensor for the detection of rebaudioside A

Bathinapatla, Ayyappa; Kanchi, Suvardhan; Singh, Parvesh; Sabela, Myalowenkosi I.; Bisetty, Krishna

doi:10.1016/j.bios.2015.09.004

Cited by 25 publications

(9 citation statements)

References 43 publications

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“…Besides carbon nanotubes [ 18 ], graphene [ 19 ], and fullerenes [ 20 ], ordered mesoporous carbons (OMC) are often tested as components of novel biosensors. Among OMC, the most frequent CMK-3 and CMK-1 are employed for biosensor matrix preparation.…”

Section: Introductionmentioning

confidence: 99%

Mesoporous carbon-containing voltammetric biosensor for determination of tyramine in food products

et al. 2016

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A voltammetric biosensor based on tyrosinase (TYR) was developed for determination of tyramine. Carbon material (multi-walled carbon nanotubes or mesoporous carbon CMK-3-type), polycationic polymer—i.e., poly(diallyldimethylammonium chloride) (PDDA), and Nafion were incorporated into titania dioxide sol (TiO2) to create an immobilization matrix. The features of the formed matrix were studied by scanning electron microscopy (SEM) and cyclic voltammetry (CV). The analytical performance of the developed biosensor was evaluated with respect to linear range, sensitivity, limit of detection, long-term stability, repeatability, and reproducibility. The biosensor exhibited electrocatalytic activity toward tyramine oxidation within a linear range from 6 to 130 μM, high sensitivity of 486 μA mM−1 cm−2, and limit of detection of 1.5 μM. The apparent Michaelis–Menten constant was calculated to be 66.0 μM indicating a high biological affinity of the developed biosensor for tyramine. Furthermore, its usefulness in determination of tyramine in food product samples was also verified.Graphical abstractDifferent food samples were analyzed to determine tyramine using biosensor based on tyrosinaseElectronic supplementary materialThe online version of this article (doi:10.1007/s00216-016-9612-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Mesoporous carbon-containing voltammetric biosensor for determination of tyramine in food products

et al. 2016

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“…The obtained results demonstrated that the IL@CoFe 2 O 4 NPs@MWCNTs@GCE exhibited the amplified electrochemical signals due to the rapid conductivity of the electrons compared to that of MWCNTs@GCE and bare GCE. Therefore, the active surface area was measured using Randles–Sevcik Equation 30 , 31 . where is the anodic peak current, n is the number of electrons transferred, C 0 is the concentration of PZM and ETB, D R is the diffusion coefficient, A is the surface area of the electrode, and V is the scan rate.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous detection of ethambutol and pyrazinamide with IL@CoFe2O4NPs@MWCNTs fabricated glassy carbon electrode

Chokkareddy

Kanchi

Inamuddin

2020

Sci Rep

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For the first time, we report a novel electrochemical sensor for the simultaneous detection of ethambutol (ETB) and pyrazinamide (PZM) using 1-ethyl-3-methylimidazolium tetrafluoroborate ([emim][Bf 4 ]) ionic liquid (iL) assimilated with multiwalled carbon nanotubes (MWcnts) decorated cobalt ferrite nanoparticles (cofe 2 o 4 nps) on the surface of glassy carbon electrode (Gce). the surface morphological and electrochemical properties of the iL@cofe 2 o 4 nps@MWcnts was characterized with X-ray diffraction (XRD), transmission electron microscope (TEM), thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR) and cyclic voltammetry (CV), differential pulse voltammetry (DPV) respectively. Moreover, the obtained results of CV demonstrated that the 9-folds enhancement in the electrochemical signals was achieved with iL@cofe 2 o 4 nps@MWcnts@Gce compared to that of a bare Gce. Additionally, the simultaneous electrochemical detection of etB and pZM was successfully accomplished using iL@cofe 2 o 4 NPs@MWCNTs over a wide-range of concentration with good limit of detection (3S/m) of 0.0201 and 0.010 μM respectively. The findings of this study identify iL@cofe 2 o 4 nps@MWcnts@Gce has promising abilities of simultaneous detection of etB and pZM in pharmaceutical formulations.

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“…Then the GO solution was prepared according to Bathinapatla et al . 17 , by quantitatively measuring 1 ml of GO into 10 ml of deionized distilled water. The ZnONPs/GO nanocomposite was achieved by mixing ZnONPs dispersion and GO dispersion in a ratio of 1:1 and ultrasonicated for 1 h to obtain a homogenized mixture.…”

Section: Computational Models and Methodsmentioning

confidence: 99%

“…Although the development of a cytochrome C biosensor for the detection of Reb-A has been previously reported in our laboratory using electrochemical and computational methods 17 , to the best of our knowledge, the development of a human T1R2 biosensor for the detection of Reb-A is yet to be reported in literature. In this study, the selection of a member of the T1R family as a candidate taste receptor was based on their physiology of taste perception embodied in the characteristics of T1R function.…”

Section: Introductionmentioning

confidence: 99%

An in-silico layer-by-layer adsorption study of the interaction between Rebaudioside A and the T1R2 human sweet taste receptor: modelling and biosensing perspectives

Arodola

Kanchi

Hloma

et al. 2020

Sci Rep

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The human sweet taste receptor (T1R2) monomer—a member of the G-protein coupled receptor family that detects a wide variety of chemically and structurally diverse sweet tasting molecules, is known to pose a significant threat to human health. Protein that lack crystal structure is a challenge in structure-based protein design. This study focused on the interaction of the T1R2 monomer with rebaudioside A (Reb-A), a steviol glycoside with potential use as a natural sweetener using in-silico and biosensing methods. Herein, homology modelling, docking studies, and molecular dynamics simulations were applied to elucidate the interaction between Reb-A and the T1R2 monomer. In addition, the electrochemical sensing of the immobilised T1R2-Reb-A complex with zinc oxide nanoparticles (ZnONPs) and graphene oxide (GO) were assessed by testing the performance of multiwalled carbon nanotube (MWCNT) as an adsorbent experimentally. Results indicate a strong interaction between Reb-A and the T1R2 receptor, revealing the stabilizing interaction of the amino acids with the Reb-A by hydrogen bonds with the hydroxyl groups of the glucose moieties, along with a significant amount of hydrophobic interactions. Moreover, the presence of the MWCNT as an anchor confirms the adsorption strength of the T1R2-Reb-A complex onto the GO nanocomposite and supported with electrochemical measurements. Overall, this study could serve as a cornerstone in the development of electrochemical immunosensor for the detection of Reb-A, with applications in the food industry.

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An ultrasensitive performance enhanced novel cytochrome c biosensor for the detection of rebaudioside A

Cited by 25 publications

References 43 publications

Mesoporous carbon-containing voltammetric biosensor for determination of tyramine in food products

Mesoporous carbon-containing voltammetric biosensor for determination of tyramine in food products

Simultaneous detection of ethambutol and pyrazinamide with IL@CoFe2O4NPs@MWCNTs fabricated glassy carbon electrode

An in-silico layer-by-layer adsorption study of the interaction between Rebaudioside A and the T1R2 human sweet taste receptor: modelling and biosensing perspectives

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