An ultrasensitive polydopamine bi-functionalized SERS immunoassay for exosome-based diagnosis and classification of pancreatic cancer

Li, Teng-Da; Zhang, Ren; Chen, Hui; Huang, Zhipeng; Ye, Xin; Wang, Hui; Deng, Anmei; Kong, Jilie

doi:10.1039/c8sc01611a

Cited by 188 publications

(140 citation statements)

References 54 publications

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“…Thus far, antibodies have been widely utilized for specific recognition of exosome biomarkers. For example, Li et al developed a novel immunoassay for the early diagnosis of pancreatic cancer, which relies on the surface‐enhanced raman scattering with ultrathin polydopamine‐encapsulated reporter . However, this approach is critically limited by factors such as instability, high‐cost, and clonal variations of antibodies.…”

Section: Discussionmentioning

confidence: 99%

Exosomes for Non‐Invasive Cancer Monitoring

Kalishwaralal

Kwon

Park

2018

Biotechnology Journal

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Exosomes, membrane‐bound phospholipid vesicles having diameters of 50–200 nm, are secreted by all cell types and circulate in human body fluids. These vesicles are known to carry cellular constituents that are specific to the originating cells (e.g., cytoplasmic/membrane proteins, RNA, and DNA). Thus, exosomes, which are both structurally stable and abundant, are robust indicators of cancers and, as a result, they have been utilized to monitor this disease in a manner that is less invasive than gold standard tissue biopsies. In this review, the history of exosomes and the specific biomarkers present in exosomes that enable accurate monitoring of various diseases are described. In addition, methods for analysis of exosomes and identification of biomarkers are presented with special emphasis being given to isolation and signaling strategies. Lastly, integrated, microfluidic systems developed for exosome‐based cancer diagnosis are described and future directions that research in this area will likely take are presented.

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Section: Discussionmentioning

confidence: 99%

Exosomes for Non‐Invasive Cancer Monitoring

Kalishwaralal

Kwon

Park

2018

Biotechnology Journal

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“…Nowadays, many analytical approaches have been exploited for sensing of cancer and infectious relatedt argets, including cancer biomarkers, cancerc ells and microorganisms. The most widely employed are polymerase chain reaction (PCR), [15] enzyme-linked immunosorbent assays (ELISA), [16] electrochemical approaches, [17] surfacee nhanced Ramans cattering (SERS) [18] and fluorescent approaches. [19] Early micromotor works in such…”

Section: Nano/micromotors For Diagnosismentioning

confidence: 99%

Nano/Micromotors for Diagnosis and Therapy of Cancer and Infectious Diseases

Yuan

Jiang

Jurado‐Sánchez

et al. 2019

Chemistry A European J

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Micromotors are man‐made nano/microscale devices capable of transforming energy into mechanical motion. The accessibility and force offered by micromotors hold great promise to solve complex biomedical challenges. This Review highlights current progress and prospects in the use of nano and micromotors for diagnosis and treatment of infectious diseases and cancer. Motion‐based sensing and fluorescence switching detection strategies along with therapeutic approaches based on direct cell capture; killing by direct contact or specific drug delivery to the affected site, will be comprehensively covered. Future challenges to translate the potential of nano/micromotors into practical applications will be described in the conclusions.

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“…In addition, nucleic acids encoding cancer‐related genetic mutation increase the interest of corresponding SERS‐based analysis over DNA and RNA . Extracellular vesicles carrying various cancer‐relevant analytes were also investigated with SERS to obtain improved diagnostics of tumor tissues . Cancer causes complex changes in the human body, which are difficult to detect with individual biomarkers especially at early stages of the disease.…”

Section: Introductionmentioning

confidence: 99%

Label‐free diagnosis for colorectal cancer through coffee ring‐assisted surface‐enhanced Raman spectroscopy on blood serum

Yan

Huang

et al. 2020

Journal of Biophotonics

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Surface-enhanced Raman spectroscopy (SERS) is garnering considerable attention for the swift diagnosis of pathogens and abnormal biological status, that is, cancers. In this work, a simple, fast and inexpensive optical sensing platform is developed by the design of SERS sampling and data analysis. The pretreatment of spectral measurement employed gold nanoparticle colloid mixing with the serum from patients with colorectal cancer (CRC). The droplet of particle-serum mixture formed coffee-ring-like region at the rim, providing strong and stable SERS profiles. The obtained spectra from cancer patients and healthy volunteers were analyzed by unsupervised principal component analysis (PCA) and supervised machine learning model, such as support-vector machine (SVM), respectively. The results demonstrate that the SVM model provides the superior performance in the classification of CRC diagnosis compared with PCA. In addition, the values of carcinoembryonic antigen from the blood samples were compiled with the corresponding SERS spectra for SVM calculation, yielding improved prediction results. K E Y W O R D S colorectal cancer diagnosis, label-free detection, machine learning, nanoparticles, SERS

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An ultrasensitive polydopamine bi-functionalized SERS immunoassay for exosome-based diagnosis and classification of pancreatic cancer

Abstract: Ultrasensitive polydopamine bi-functionalized surface enhanced Raman scattering immunoassay expedites exosome-based diagnosis and classification of pancreatic cancer.

Cited by 188 publications

References 54 publications

Exosomes for Non‐Invasive Cancer Monitoring

Exosomes for Non‐Invasive Cancer Monitoring

Nano/Micromotors for Diagnosis and Therapy of Cancer and Infectious Diseases

Label‐free diagnosis for colorectal cancer through coffee ring‐assisted surface‐enhanced Raman spectroscopy on blood serum

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