An Ultrastructural and Cytochemical Study of Membrane Alterations in X-Irradiated Liver Tissue from Normal and Vitamin E-Deficient Ducklings

Huijbers, W. A. R.; Oosterbaan, J. A.; Meskendorp-Haarsma, T. J.; Hardonk, Mj; Molenaar, I.

doi:10.2307/3574975

Cited by 9 publications

(4 citation statements)

References 35 publications

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“…Also the present results revealed dilation in the rough endoplasmic reticulum induced from exposure to 6 Gy of gamma-radiation. Similar changes were also reported by Huijbers et al (1979) who reported that, the damage of the rough endoplasmic reticulum (RER) reflects the impairment of protein synthesis in such cells. In the present study whole body -irradiation in rats induced damage in the proximal and distal convoluted tubules and showed destruction of brush boarder and microvilli.…”

Section: Discussionsupporting

confidence: 86%

“…In the present study whole body -irradiation in rats induced damage in the proximal and distal convoluted tubules and showed destruction of brush boarder and microvilli. These changes could be attributed to the degenerative changes involving the whole tubules as well as to thickening of tubular basement membrane, in accordance with Huijbers et al (1979) and Cohen (2000). Also, ultra-structural studies carried on gamma-irradiated albino rats showed that, focal tubular alterations were detected (Mulder et al 2002).…”

Section: Discussionmentioning

confidence: 85%

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Protective Effect of Β-Carotene on Gamma Radiation- Induced Renal Histological and Ultrastructural Changes in Male Albino Rats

Abunour

Rady

Khalil

2008

Al-Azhar Bulletin of Science

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The present work was conducted to investigate the histological and ultra-structural changes in the kidney tissues of male albino rats due to the exposure of gamma-radiation and to investigate tissue-protection from the damaging effects of gamma-radiation using a natural product namely, Beta-carotene. The histological studies revealed progressive pathological lesions in the kidney of whole body γ-irradiated rats; as represented by severe damage in glomerular tuft and increase in masengal cells with wide Bowman's space and sever hemorrhagic areas between degenerated renal tissue, and renal tubules exhibited various degrees of degeneration. The ultra-structural studies revealed that epithelial cells lining the proximal and distal convoluted tubules exhibited an increase in the swelling mitochondria, dilation in the rough endoplasmic reticulum and electron dense in the nuclear chromatin. Thickness in basement membrane, dilation of infolded, and damage of brush border of the proximal tubules were also observed. The treatment with β-carotene pre-exposure to gamma radiation attenuated most of these changes. Therefore, the present study has implication for the potential use of β-carotene as a radioprotector.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 85%

Protective Effect of Β-Carotene on Gamma Radiation- Induced Renal Histological and Ultrastructural Changes in Male Albino Rats

Abunour

Rady

Khalil

2008

Al-Azhar Bulletin of Science

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“…Also the present results revealed dilations in the rough endoplasmic reticulum induced from exposure to 6 Gy of gamma-radiation. Similar changes were also reported by Huijbers et al (1979) who observed that, the rough endoplasmic reticulum represent the main site of protein synthesis in the cells, and thus the damage of the rough endoplasmic reticulum (RER) reflect the impairment of protein synthesis in such cells. In the present study whole body gamma-irradiation of rats induced damage in the proximal and distal convoluted tubules and showed destruction of brush border and microvilli.…”

Section: Discussionsupporting

confidence: 86%

Protective Effects of Β-Carotene on Gamma Radiation-Induced Renalhistological and Ultrastructural Changes in Albino Rats

Seham¹,

Rady

Ihab³

2008

The International Conference on Chemical and Environmental Engi

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The present work was conducted to investigate the histological, and ultra-structural changes that occurred in the kidney tissues of albino rats due exposure to gammaradiation. Also we investigated how to protect the tissues from the damaging effects of gamma radiation using a natural product such as Beta-carotene. Thirty four male albino rats were divided into four main groups; the first group served as control (5 rats). Rats of the second group (5 rats) ) received 5mg/kg body wt. of β-carotene at a daily oral dose for 2 consecutive weeks .The rats of the third main group (12 rats) were exposed to a sub-lethal dose (6Gy) of whole body γ-irradiation and then divided into four equal subgroups. Rats of the fourth main group (12 rats) received βcarotene before being exposed to gamma radiation and then divided into four equal subgroups. Treated rats were autopsied at four intervals; one day, one week, two weeks and four weeks post irradiation. The histological studies of the groups that exposed to gamma radiation revealed progressive pathological lesions as represented by severe damage in glomerular tuft with wide Bowman's space and severe hemorrhagic areas scattered in between degenerated renal tissues, increase in mesangial cells and renal tubules which suffered various degrees of degeneration. The ultra-structural studies revealed that epithelial cells lining the proximal and distal convoluted tubules showed an increase in the swollen mitochondria , dilation in the rough endoplasmic reticulum and electron dense in the nuclear chromatin. Thickness in basement membrane and dilation of infolded were observed. Damage of brush boarder and collagen fibers was also observed. The treatment with β-carotene (5mg/kg b. wt. for two consecutive weeks) pre-exposure to gamma radiation attenuated most of these changes. Therefore, the present study has implication for the potential use of β-carotene as a radioprotector.

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“…The literature on experimental nutritional myopathy in ducklings always refers to dayold birds kept on a vitamin E-deficient diet for a maximal period of 1-4 weeks from the day of hatching [5,7,9,[12][13][14]21]. The sud den onset of this symptom is usually ob served in the 2nd or 3rd week and inevitably leads to death of the animal within one or a few more days.…”

Section: Introductionmentioning

confidence: 99%

Nutritional Myopathy in Ducklings: a Growth Rate-Dependent Symptom of ‘Tissue Peroxidosis’ due to a Net Nutritional Shortage of Vitamin E plus Selenium in Skeletal Muscle

Vos

Hulstaert

Molenaar³

1981

Ann Nutr Metab

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Although low dietary vitamin E is an absolute prerequisite for the occurrence of nutritional myopathy in ducklings, it was found that the myopathy can be prevented if the vitamin E depletion does not start before 2 weeks of age and if the dietary selenium content is not too low (≧ 74 ppb). Under these circumstances growth remained normal and the animals stayed alive during the experimental period of 14 weeks, despite low levels of vitamin E in serum, liver, and muscle. The weekly growth rate of the nondeficient duckling, as derived from the growth curve, is extremely high during the first 2 weeks of age with a distinct peak during the 2nd week followed by a steep fall during the 3rd week. In vitamin E-deficient ducklings tissue vitamin E dilution curves were calculated by mathematic transformation of the growth curve. The results suggest that a critical net nutritional shortage of vitamin E plus selenium in skeletal muscle causes myopathy in ducklings. It is proposed that nutritional myopathy should be considered as a symptom of’tissue peroxidosis’ and not as a symptom of vitamin E deficiency per se.

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An Ultrastructural and Cytochemical Study of Membrane Alterations in X-Irradiated Liver Tissue from Normal and Vitamin E-Deficient Ducklings

Cited by 9 publications

References 35 publications

Protective Effect of Β-Carotene on Gamma Radiation- Induced Renal Histological and Ultrastructural Changes in Male Albino Rats

Protective Effect of Β-Carotene on Gamma Radiation- Induced Renal Histological and Ultrastructural Changes in Male Albino Rats

Protective Effects of Β-Carotene on Gamma Radiation-Induced Renalhistological and Ultrastructural Changes in Albino Rats

Nutritional Myopathy in Ducklings: a Growth Rate-Dependent Symptom of ‘Tissue Peroxidosis’ due to a Net Nutritional Shortage of Vitamin E plus Selenium in Skeletal Muscle

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An Ultrastructural and Cytochemical Study of Membrane Alterations in X-Irradiated Liver Tissue from Normal and Vitamin E-Deficient Ducklings

Cited by 9 publications

References 35 publications

Protective Effect of Β-Carotene on Gamma Radiation- Induced Renal Histological and Ultrastructural Changes in Male Albino Rats

Protective Effect of Β-Carotene on Gamma Radiation- Induced Renal Histological and Ultrastructural Changes in Male Albino Rats

Protective Effects of Β-Carotene on Gamma Radiation-Induced Renalhistological and Ultrastructural Changes in Albino Rats

Nutritional Myopathy in Ducklings: a Growth Rate-Dependent Symptom of &lsquo;Tissue Peroxidosis&rsquo; due to a Net Nutritional Shortage of Vitamin E plus Selenium in Skeletal Muscle

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Nutritional Myopathy in Ducklings: a Growth Rate-Dependent Symptom of ‘Tissue Peroxidosis’ due to a Net Nutritional Shortage of Vitamin E plus Selenium in Skeletal Muscle